Untersuchungen zum Energiestoffwechsel der humanen Hornhaut in verschiedenen Kultursystemen

Abstract

The severe anaerobic situation in Optisol stored corneas leads to a lack in energy recruitment. This reduces the ability of cell function (mitosis) and the function of the monolayer (migration, elimination). Whether these changes are reversible after transplantation has to be determined in future.

Preservation of canine and feline corneoscleral tissue in Optisol® GS

“…The gaps in the endothelial cell layer were limited to the size of a single cell, and there was no significant decrease in cell density, which remained well above 2000 cell/mm 2 . These findings were similar to those of porcine corneoscleral tissue that had been stored for 13 days 33 and human corneoscleral tissue stored for 14-15 days. 1,17 Van Horn et al 7 has already reported that the endothelial cell layers of feline and human corneosceral tissue react similarly during storage, and they proposed the cat as an experimental model in human studies of the cornea.…”

“…29 Similarly, Redbrake et al . 33 did not find any significant difference in cell densities of control tissue and human corneal tissue that had been stored in Optisol ® GS for 13 days. However, they cautioned that it was not possible to accurately assess the loss of endothelial cells during storage, because dead or damaged cells cannot be eliminated from the endothelial layer at 4 °C.…”

“…In addition, the cell density decreased significantly from that of the control tissue and that of canine tissue after 10 days of storage. In contrast, Redbrake et al 33 reported that there was no significant decrease in endothelial cell density of human corneoscleral tissue after 13 days of storage. In other studies involving storage of human corneoscleral tissue in Optisol ® GS, no significant decrease in cell density was seen after 14-15 days.…”

“…The decrease in cell density after 10 days of storage was not considered significant, because cell density still exceeded 2000 cells/mm 2 29 . Similarly, Redbrake et al 33 . did not find any significant difference in cell densities of control tissue and human corneal tissue that had been stored in Optisol ® GS for 13 days.…”