Untersuchungen zum Nachweis antithrombozytärer Heteroantikörper durch Immunfluoreszenz

Mueller-Eckhardt, C; Sachs, V.

doi:10.1055/s-0038-1655599

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same applies to immunofluorescence microphotometry (Denk et al, 1969;van Boxtel et a / , I973a, b, 1975). The development of an ordinary immunofluorescence test on platelets in suspension was hampered by the nonspecific fluorescence that is often obtained (Mueller-Eckhardt & Sachs, 1966;Mueller-Eckhardt, 1977).…”

Section: Discussionmentioning

confidence: 99%

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

et al. 1978

View full text Add to dashboard Cite

Immunofluorescence tests on platelets have always been hampered by nonspecific fluorescence caused by non-immunological binding of plasma proteins to the platelet membrane. It was found that this could be easily overcome by fixation of the cells with paraformaldehyde (PFA). By using PFA-fixed platelets, a simple method for the detection of platelet antibodies, the platelet suspension immunofluorescence test (PSIFT) was developed. PFA fixation did not alter or inactivate the platelet antigens tested. Platelet-reactive antibodies detected specifically with the PSIFT included platelet-specific agglutinins of the IgM class, non-agglutinating platelet-specific antibodies of the IgG class, drug-dependent platelet antibodies, HLA antibodies, as well as anti-A and anti-B antibodies. The sensitivity of the new test was satisfactory, as was its reproducibility. Measurement of platelet immunofluorescence was possible in a continuous flow microfluorometer, making a principle, quantitation of platelet antibodies and antigens possible.

show abstract

Section: Discussionmentioning

confidence: 99%

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

et al. 1978

View full text Add to dashboard Cite

show abstract

“…Aspecific fluorescence is a major problem, particularly when the technique is used on platelets (3,15,20). The limited possibilities for quantification of the results also form an impediment to closer analysis of the optima!…”

mentioning

confidence: 99%

Immunofluorescence Microphotometry for the Detection of Platelet Antibodies. I. Standardization of the Method

1973

View full text Add to dashboard Cite

The standardization is described of an immunofluorescent microphotometric method that offers a solution for the problems generally encountered in immunofluorescence procedures on platelets. Fluorescein isothiocyanate (FITC) conjugated .anti‐immunoglobulin serum and anti‐IgG serum were used. Sera, containing isoor hetero‐antibodies against platelets that had given positive reactions with one or more of the conventional serological techniques, were used as positive controls. The method described proved to have a good reproducibility. Significant differences in fluorescence were obtained using the above‐mentioned positive sera and normal control sera. It was found that platelets stored in sodium‐azide could be used as antigen. A number of variables that may influence the difference in fluorescence obtained with positive and normal sera were studied.

show abstract

Untersuchungen zum Nachweis antithrombozytärer Heteroantikörper durch Immunfluoreszenz

Cited by 2 publications

References 0 publications

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

A Simple Immunofluorescence Test for the Detection of Platelet Antibodies

Immunofluorescence Microphotometry for the Detection of Platelet Antibodies. I. Standardization of the Method

Contact Info

Product

Resources

About