Unusual anti-leukemia activity of nanoformulated naproxen and other non-steroidal anti-inflammatory drugs

Kumar, Raj; Siril, Prem Felix; Javid, Farideh A.

doi:10.1016/j.msec.2016.08.024

Cited by 53 publications

(25 citation statements)

References 70 publications

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“…Cells were seeded into 96-well plates at 2000 cells per well. After 24 hours of incubation, cells were treated with varying doses of fluoxetine hydrochloride (1 nM to 100 µM) or vehicle (sterile water) for 96 h, after which the MTT assay was performed as previously reported (Kumar et al, 2016;Blackburn et al, 2016) by addition of 10 µl of MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide). Following 4 hour incubation with MTT, the content of each well was removed and the formazan crystals were dissolved in dimethylsulfoxide, DMSO (150 µL).…”

Section: 2cell Viability Assaymentioning

confidence: 99%

Fluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells

Marcinkute

Afshinjavid

Fatokun

et al. 2019

European Journal of Pharmacology

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Fluoxetine has been shown to induce anti-tumour activity. The aim of this study was to determine the effect of fluoxetine on HCT116+/+ and p53 gene-depleted HCT116-/-human colorectal cancer cells and the mechanisms, including potential p53-dependence, of its action. Fluoxetine-induced apoptosis was investigated by mitochondrial membrane potential assay, Annexin V assay, two-step cell cycle analysis using NC-3000™ system and pharmacological inhibition assays. Fluoxetine induced very selectively concentration-dependent apoptosis in human colorectal cancer cells by altering mitochondrial membrane potential and inducing translocation of phosphatidylserine to the outer membrane layer. Further evidence of the preponderance of apoptosis in fluoxetine-induced cell death is provided by the finding that the cell death was not blocked by inhibitors of parthanatos, a form of cell death that results from overactivation of the enzyme poly (ADP-ribose) polymerase (PARP) but is different from apoptosis. Data obtained indicate fluoxetine caused cell cycle event at Sub-G1 and G0/G1 phases in both cell lines. In terms of apoptosis, there is no significant difference between the responses of the two cell lines to fluoxetine. In conclusion, fluoxetine's cytotoxicity induces mainly apoptosis and causes DNA fragmentation in human colorectal cancer cells, which seemed to be independent of the p53 protein, as no significant difference in death profiles in response to fluoxetine treatment was observed in both the p53-intact and the p53-deleted cell lines. Fluoxetine, therefore, has potential for being repurposed as a drug for the treatment of colon cancer and thus deserves further investigations in this context.

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Section: 2cell Viability Assaymentioning

confidence: 99%

Fluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells

Marcinkute

Afshinjavid

Fatokun

et al. 2019

European Journal of Pharmacology

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“…We have recently developed the evaporation-assisted solvent–antisolvent interaction (EASAI) method by enhancing the rate of supersaturation. 34,35 Nanoparticles of HECs (RDX and HMX) were prepared by the EASAI method using acetone as solvent at 70 °C. 36 This method is not only limited to HECs but also useful for the nanoformulation of different simple organic compounds.…”

Section: Introductionmentioning

confidence: 99%

Engineering the Morphology and Particle Size of High Energetic Compounds Using Drop-by-Drop and Drop-to-Drop Solvent–Antisolvent Interaction Methods

2019

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Morphology-controlled precipitation of three powerful organic high energetic compounds (HECs) viz. cyclotrimethylenetrinitramine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and 2-methyl-1,3,5-trinitrobenzene (TNT) was achieved by two different processes, namely, drop-by-drop (DBD) and drop-to-drop (DTD) solvent–antisolvent interaction methods. Effect of different experimental parameters on the mean size and morphology of the prepared submicron-sized particles of HECs was investigated thoroughly. The DBD method favors the formation of nanosized particles of RDX and TNT at lower concentrations (5 mM). However, a significant increase in the mean particle size occurred at higher concentrations (25 and 50 mM). Formation of facetted crystals of RDX, HMX, and nanorods of TNT was observed at higher concentrations because of the interaction of crystal facets with the antisolvent. Relatively, smaller sized, spherical particles of RDX and HMX could be prepared through the DTD method even at higher concentrations (25 mM). The DTD method is a continuous process and hence is a facile method for industrial applications. X-ray diffraction and Fourier transform infrared spectroscopy studies revealed that RDX, HMX and TNT were precipitated in their most stable polymorphic forms α, β, and monoclinic, respectively. Differential scanning calorimetry showed that the thermal response of the nano-HECs was similar to the respective raw-HECs. A slight decrease in crystallinity and the melting point was observed because of the decrease in the mean particle size.

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“…In bottom‐up approach, reprecipitation or antisolvent precipitation or liquid antisolvent precipitation is a simple method for the preparation of organic nanoparticles. Antisolvent reprecipitation is widely used nanoformulation technique for organic compounds such as poorly water soluble APIs, polymers, dyes, and pigments . In the antisolvent reprecipitation process, nanoparticles were formed by mixing of the solution of substance with suitable antisolvent under stirring .…”

Section: Ff Nanoformulation Techniques and Literature Reportsmentioning

confidence: 99%

Solubility and Bioavailability of Fenofibrate Nanoformulations

Kumar

2020

ChemistrySelect

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Dedicated to my parents, family, all my teachers and friends.Fenofibrate is a poorly water soluble lipid-regulating drug used to control triglyceride and cholesterol levels in blood plasma. However, due to its poor aqueous solubility, it has poor therapeutic effectiveness. Recently, several approaches based on nanotechnology have been developed to enhance the water solubility, dissolution, bioavailability, and control release of Fenofibrate. However, very few of these innovative formulations have reached the stage of clinical trials and only few have commercialized. Here, we systematically review the techniques such as milling, antisolvent precipitation, sonication, supercritical fluid techniques, electrospray, self-emulsifying drug delivery, lipid-based nanoparticle, and silica nanostructurebased formulation used to enhance bioavailability of Fenofibrate. We have reviewed the progress in Fenofibrate nanoformulations, commercial products, and future challenges for further investigation.[a] Dr.

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Unusual anti-leukemia activity of nanoformulated naproxen and other non-steroidal anti-inflammatory drugs

Cited by 53 publications

References 70 publications

Fluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells

Fluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells

Engineering the Morphology and Particle Size of High Energetic Compounds Using Drop-by-Drop and Drop-to-Drop Solvent–Antisolvent Interaction Methods

Solubility and Bioavailability of Fenofibrate Nanoformulations

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