Unusual glycosides of pyrrole alkaloid and 4′-hydroxyphenylethanamide from leaves of Moringa oleifera

Moringa oleifera is a tropical plant with high nutritional and medicinal value. Recent studies have reported its remarkable effects in inflammatory, antioxidative, and anti‐diabetes modulations, but there was no significant report on its role in animal breeding. In this study, we investigated the effects of dietary Moringa oleifera leaf (MOL) on reproductive performances in mice. We studied the reproductive performance of mice for six consecutive gestations. Mice fed with 4% MOL diet showed improved litter size, litter birth weight, and litter survivals until weaning age compared to control mice fed with normal diet (p < 0.05). Mice fed with MOL diet did not change weight and organ coefficients. Serum malondialdehyde (MDA) concentrations in both male and female mice were significantly decreased by dietary MOL (p < 0.05), but glutathione peroxidase (GSH‐PX) and superoxide dismutase (SOD) were unchanged. For male, dietary MOL significantly reduced sperm abnormality rate (p < 0.05) and Bcl2‐associated X protein (Bax) expression in testis (p < 0.05), but did not affect serum testosterone and the expression levels of androgen receptor (AR), phosphoglycerate kinase 2 (Pgk2), protamine2 (Prm2), and B cell leukemia/lymphoma 2 (Bcl2) in testis. For female, dietary MOL did not change serum estradiol and the expressions of estrogen receptor beta (ERβ), Bcl2, Bax, and vascular endothelial growth factor receptor (VEGFR) in ovary. In summary, MOL increased litter size and antioxidant ability, reduced the rate of sperm abnormality and the expression of Bax. Therefore, MOL may serve as a functional feed addictive for improving animal reproductive performance.

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“…(Sahakitpichan, Mahidol, Disadee, Ruchirawat, & Kanchanapoom, 2011). A possible reason is that 8% MOL feed contain higher alkaloids.…”

Section: Discussionmentioning

confidence: 99%

The beneficial effects of Moringa oleifera leaf on reproductive performance in mice

Zeng

Luo

Wang

et al. 2019

Food Science & Nutrition

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“…Many numbers of research have been conducted as to M. oleifera, many publications presented biological activity and components from the leaves of M. oleifera. 4,5,[19][20][21][22][23][24][25][26] In the present study, we examined the effects of Moringa oleifera fruits and its main flavonoids on the growth of HCT116 human colon cancer cells using MTT, Nuclear staining with Hoechst 33258 and Western blotting analysis.…”

Section: Resultsmentioning

confidence: 99%

Induction of Apoptosis withMoringa oleiferaFruits in HCT116 Human Colon Cancer Cells Via Intrinsic Pathway

Guon

Chung

2017

Nat Prod Sci

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− Moringa oleifera Lam (M. oleifera, Moringaceae) is a tree of the Moringaceae family that can reach a height of between 5 and 10 m. The current paper presents cytotoxic effect of M. oleifera fruits and its flavonoids 1 and 2. The viability of HCT116 human colon cancer cells were 38.5% reduced by 150 µg/mL of ethanolic extracts in a concentration-dependent manner; in addition, we observed the apoptotic features of cell shrinkage and decreased cell size. Bcl-2 family proteins were regulated as determined by Western blotting analysis, suggesting that M. oleifera fruits and their flavonoids 1 and 2 induced apoptosis through an intrinsic pathway. Based on our findings, 70% ethanolic extracts of M. oleifera fruits and flavonoids 1 and 2 might be useful as cytotoxic agents in colorectal cancer therapy.

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“…In Marumoside A, the aglycone moiety is structurally related to potential antiinflammatory active Capsaicin and hydroxyphenylacetamides, the bioactivity of these compounds strictly depend on the fatty amine moiety. In the present study, the antiinflammatory activities of all the synthesized compounds (14)(15)(16)(17)(18)(19)(20)(21)(22)(23) were tested and the inhibition of TNF-α and IL-1β secretion as a measure of anti-inflammatory activity was monitored. The data to this regard are presented in Tables 2 and 3. Antioxidant activity: Marumoside A and its synthesized derivatives were tested for the DPPH free radical scavenging activity (see Table 4).…”

Section: Biology: Antiinflammatory Activitymentioning

confidence: 99%

“…Recently, Marumoside A and other compounds were isolated from the leaves of Moringa oleifera of Thai origin 18 . In Marumoside A, 4-hydroxyphenylethanamide was glycosylated at anomeric hydroxyl group of Lrhamnose in α-configuration.…”

mentioning

confidence: 99%

Untitled

2016

IJPSR

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ABSTRACT:In the present study, Marumoside A isolated from Moringa oleifera, a traditional medicinal plant, was synthesized for the first time using trichloroacetimidate donor as a key step. The aglycone 4-hydroxy phenylacetamide was prepared from 4-hydroxyphenyl acetic acid using oxallyl chloride and aqueous ammonia. The lipid derivatives of Marumoside A were synthesized using different fatty amines. The anti-inflammatory activity of the Marumoside A and its lipid derivatives was evaluated for the inhibition of TNF-α and IL-1β secretion levels. Among all the synthesized molecules, the oleoyl amine lipid (unsaturated) derivative showed significant inhibition of TNF-α and IL-1β secretion with IC 50 value of 16.7 µM and 23.4 µM, respectively, when compared to Marumoside A and its saturated lipid derivatives. Further, the Marumoside A and its lipid derivatives also showed DPPH radical scavenging activity.

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Unusual glycosides of pyrrole alkaloid and 4′-hydroxyphenylethanamide from leaves of Moringa oleifera

Cited by 80 publications

References 17 publications

The beneficial effects of Moringa oleifera leaf on reproductive performance in mice

The beneficial effects of Moringa oleifera leaf on reproductive performance in mice

Induction of Apoptosis withMoringa oleiferaFruits in HCT116 Human Colon Cancer Cells Via Intrinsic Pathway

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