Unusual phenotype of B cells in the thymus of normal mice.

Miyama-Inaba, Muneo; Kuma, Sho-ichi; Inaba, Kayo; Ogata, Hiroaki; Iwai, Hiroshi; Yasumizu, Ryoji; Muramatsu, Shigeru; Steinman, R M; Ikehara, Susumu

doi:10.1084/jem.168.2.811

Cited by 112 publications

(61 citation statements)

References 15 publications

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“…Interestingly, although the thymus is known to contain a minor subpopulation of B lymphocytes (31)(32)(33)(34), this defect was only observed when bone marrow cells from young NZB donors were used to repopulate NZB thymuses. No differences in thymic B cell frequency were demonstrable when NZB donor cells were derived from 6-mo-old mice or when NZB donor cells of any age were seeded into S/W lobes.…”

Section: Cd8mentioning

confidence: 99%

NZB Mice Exhibit a Primary T Cell Defect in Fetal Thymic Organ Culture

Hashimoto

Dorshkind

Montecino‐Rodriguez

et al. 2000

The Journal of Immunology

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Defects in T cell development have been suggested to be a factor in the development of systemic autoimmunity in NZB mice. However, the suggestion of a primary T cell defect has often been by extrapolation, and few direct observations of T cell precursors in NZB mice have been performed. Moreover, the capacity of NZB bone marrow T cell precursors to colonize the thymus and the ability of the NZB thymic microenvironment to support T lymphopoiesis have not been analyzed. To address this important issue, we employed the fetal thymic organ culture system to examine NZB T cell development. Our data demonstrated that NZB bone marrow cells were less efficient at colonizing fetal thymic lobes than those of control BALB/c or C57BL/6 mice. In addition, NZB bone marrow cells did not differentiate into mature T cells as efficiently as bone marrow cells from BALB/c or C57BL/6 mice. Further analysis revealed that this defect resulted from an intrinsic deficiency in the NZB Lin−Sca-1+c-kit+ bone marrow stem cell pool to differentiate into T cells in fetal thymic organ culture. Taken together, the data document heretofore unappreciated deficiencies in T cell development that may contribute to the development of the autoimmune phenotype in NZB mice.

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Section: Cd8mentioning

confidence: 99%

NZB Mice Exhibit a Primary T Cell Defect in Fetal Thymic Organ Culture

Hashimoto

Dorshkind

Montecino‐Rodriguez

et al. 2000

The Journal of Immunology

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“…The proliferative assays were carried out as previously described (1). Assays for Iblyclonal Ig Secretions.…”

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confidence: 99%

Functional analyses of thymic CD5+ B cells. Responsiveness to major histocompatibility complex class II-restricted T blasts but not to lipopolysaccharide or anti-IgM plus interleukin 4.

Inaba

Adachi

et al. 1990

The Journal of Experimental Medicine

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In a previous report, we demonstrated that a small number of B cells are present in the thymus ofnormal mice, and that the majority ofthymic B cells show the phenotype observed for Ly-1 + (CD5 +) B cells in other tissues (1). Thus, the majority of thymic B cells express surface CD5, IgM, B220 (CD45R), and Mac-1(CDllb), and a lower amount of MHC class II relative to peripheral B cells. The functions of the CD5+ subset and the minor CD5-component in the thymus have not been determined, however, because of prior difficulties in isolating these B cells.In this paper, we use a recent method for isolating thymic B cells to show that the CD5 + subset has low responsiveness to B cell stimulants, such as LPS or IL-4, but is induced to grow and make antibody after a direct interaction with MHC class II-reactive T blasts .

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“…+ CD19 + CD5 + CD1d hi IL-10 + B regs are present in the thymus Thymic B cells have been proposed to play a critical role in T cellnegative selection [22,23]. To this end, we determined whether there exists a population of B regs in murine thymus.…”

Section: B220mentioning

confidence: 99%

“…In this regard, B cells have been proposed to play a critical function in T cell-negative selection [22,23]. Thymic B cells preferentially reside at the junction of the thymic cortex and the medulla, an area thought to be where negative selection occurs.…”

mentioning

confidence: 99%