Unusually high frequency of a 69-bp deletion within the carboxy terminus of the LMP-1 oncogene of Epstein–Barr virus detected in Burkitt’s lymphoma of Turkish children

Taçyıldız, Nurdan; Çavdar, Ayhan O.; Ertem, Ulya; Oksal, Ayşegül; Kutluay, Lale; Uluoğlu, Ömer; Lin, Jung‐Chung

doi:10.1038/sj.leu.2401203

Cited by 21 publications

(23 citation statements)

References 21 publications

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“…The high frequency of Xho1 polymorphism found in nasopharyngeal carcinoma LMP-1, as shown in this study and those reported previously from Asia, suggest that these mutations are specific markers for the Asian nasopharyngeal carcinoma as such mutations were not found in other endemic regions such as Africa. A larger deletion detected in one of our nasopharyngeal carcinoma throat wash samples may be similar to the 69-bp deletion reported by Tacyildiz et al [1998], in Turkish children with Burkitt lymphoma. The exact size of this deletion could not be determined without sequencing.…”

Section: Discussionsupporting

confidence: 86%

“…A strain from Alaska shared some of the amino terminal base changes with the China 1 strain, but retained the carboxyl terminal 30 bp and harbored an additional 15 nucleotide changes [Miller et al, 1994]. Viral isolates from Japan also recorded a high prevalence of the carboxyl terminal 30-bp deletion [Itakura et al, 1996] and from Turkey, a high frequency of a 69-bp deletion was reported [Tacyildiz et al, 1998]. …”

Section: Introductionmentioning

confidence: 99%

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Analyses of Epstein‐Barr virus latent membrane protein‐1 in Malaysian nasopharyngeal carcinoma: High prevalence of 30‐bp deletion, Xho1 polymorphism and evidence of dual infections

Tan¹,

Peh²,

Sam³

2002

Journal of Medical Virology

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Nasopharyngeal carcinoma, a malignancy associated closely with Epstein-Barr virus (EBV), is prevalent among Chinese of Southern China origin. Epidemiological studies indicate a high prevalence of EBV in Asia with viral isolates having typical characteristics of the putative viral oncogene, latent membrane protein 1 (LMP-1), such as the loss of the Xho1 restriction site in Exon 1 and the 30-bp deletion in Exon 3. The EBV LMP-1 gene from throat washings of 120 nasopharyngeal carcinoma patients and 14 healthy individuals were analyzed. Similar analyses were also carried out on 30 and 12 postnasal space biopsies from nasopharyngeal carcinoma patients and healthy individuals, respectively. The 30-bp deletion was detected in 20% of nasopharyngeal carcinoma throat washes and in 100% of nasopharyngeal carcinoma postnasal space biopsies. Interestingly, 16% of the nasopharyngeal carcinoma biopsies possessed both the deleted and the undeleted variants, suggestive of dual infections. The notion of dual infections in nasopharyngeal carcinoma was further supported by the coexistence of both "F" and "f" (BamH1F region) EBV variants in 11% of the nasopharyngeal carcinoma biopsies. All of the throat washes and biopsies from the healthy controls showed the undeleted variant. The loss of the Xho1 restriction site was found with higher frequency both in throat washes and biopsies from patients with nasopharyngeal carcinoma. The discrepancy in the frequency of the 30-bp deletion between throat washes (20%) and postnasal space biopsies (100%) was an indication that this deletion is specific for viral isolates from primary tumour sites.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Analyses of Epstein‐Barr virus latent membrane protein‐1 in Malaysian nasopharyngeal carcinoma: High prevalence of 30‐bp deletion, Xho1 polymorphism and evidence of dual infections

Tan¹,

Peh²,

Sam³

2002

Journal of Medical Virology

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“…Ertem et al found serum EBV-VCA-IgG positivity in all 63 pediatric BL patients included in the study 10 . Due to high incidence of EBV positivity in BL cases in Turkey and due to abdominal localization of some cases, these cases were suggested to be neither African nor American type, but an "intermediate form" [9][10][11][12]14 .…”

Section: Discussionmentioning

confidence: 99%

Pediatrik lenfomalarda histopatolojik subtip ve Ebstein-Barr virus ilişkisi: immünohistokimyasal ve in situ hibridizasyon çalışması

Bağır

Acikalin

Ergïn

et al. 2018

Cukurova Medical Journal

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AbstractÖz Purpose: The aim of this study was toexplore the histopathologic subtypes of pediatric lymphomas and their relation with Ebstein-Barr virus infection in our region. Materials and Methods:In this retrospective study, 87 children including 36 cases with Hodgkin lymphoma and 51 cases with non-Hodgkin lymphoma were included in the study. The pathologic slides were used to investigate immunohistochemical staining with ebstein-barr virüs latent membrane protein-1 and in-situ hybridization. Results:The most common histopathological subtype in hodgkin lymphoma cases was mixed cellular classical hodgkin lymphoma (55.6%). The most common histopathological subtype in Non-Hodgkin lymphoma cases was Burkitt lymphoma (51.0%). Ebstein-Barr virüs latent membrane protein-1 was positive in 29 Hodgkin lymphoma. All non-Hodgkin lymphoma cases were stained negative with ımmunohistochemical. The positive staining rate of Hodgkin lymphoma cases with in-situ hybridization was 83.3% and this rate was 27.5% in nonHodgkin lymphoma cases. The differences between groups for both staining methods were significant. Conclusion:The results of this study show that the distribution of histopatholojik subtypes of pediatric lymphomas similar to in developing countries. And we have observed that in-situ hybridization is more specific than immunohistochemistry because Ebstein-Barr virus positivity is more detected in in-situ hybridization.Amaç: Bu çalışmanın amacı bölgemizdeki pediatrik lenfomaların histopatolojik alt tiplerinin oranını ve bunların Ebstein-Barr virüs enfeksiyonu ile olan ilişkisini incelemektir. Gereç ve Yöntem: Hodgkin lenfoma tanısı alan 36 olgu ve non-hodgkin lenfoma tanısı alan 51 olgu retrospektif arşiv taraması yapılarak çalışmaya dahil edildi. Olgulara immünohistokimyasal olarak Ebstein-Barr virüs latent membran proteini-1 ve in-situ hibridizasyon uygulandı. Bulgular: Hodgkin lenfoma olgularında; en sık rastlanan histopatolojik alt tipler sırası ile mikst sellüler klasik hodgkin lenfoma (%55,6) idi. Non-Hodgkin lenfoma olgularında en sık rastlanan histopatolojik alt tip Burkitt lenfoma (%51) idi. Ebstein-Barr virüs latent membran proteini-1 ile 29 hodgkin lenfoma olgusunda pozitif iken non-hodgkin lenfoma olgularında immünohistokimya ile pozitif boyanma saptanmadı. Hodgkin lenfoma olgularında in situ hibridizasyon ile boyanma oranı % 83.,3 iken nonHodgkin lenfoma olgularında bu oran % 27.5 idi.. Her iki boyama yöntemi için gruplar arasındaki fark istatistiksel olarak anlamlıydı. Sonuç: Bu çalışmada, olgularımızın histopatolojik dağılımlarının gelişmekte olan ülkelerdeki pediatrik lenfoma olguları ile benzer olduğunu göstermektedir. Aynı zamanda in situ hibridizasyon'da Ebstein-Barr virüs pozitifliği daha fazla olması nedeniyle in situ hibridizasyonun ebstein barr virüs tespitinde immünohistokimyadan daha spesifik olduğunu düşünmekteyiz.

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“…LMP1 variant was identified by PCR using P5 and P6 primers specific to the DNA sequence spanning the 30 bp deletion region in the C-terminus of the LMP1 gene [Tacyildiz et al, 1998]. Amplification was performed with 5 ml of DNA in 50 ml of reaction mixture containing: 1Â PCR Gold Buffer (Applied Biosystems), 1.5 mM MgCl 2 (Applied Biosystems), 200 nM each of two primers, 200 mM each dNTP, and 2.5 U AmpliTaq Gold polymerase (Applied Biosystems).…”

Section: Lmp1 Variant Analysismentioning

confidence: 99%

Epstein–Barr virus gene expression and latent membrane protein 1 gene polymorphism in pediatric liver transplant recipients

Kasztelewicz

Jankowska

Pawłowska

et al. 2011

Journal of Medical Virology

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Immunosuppressed pediatric transplant recipients are at risk of developing Epstein-Barr virus (EBV)-associated complications (such as post-transplant lymphoproliferative disorders). Monitoring of the EBV DNA level in blood alone has a low predictive value for the post-transplant course of EBV infection and its complications. Therefore, additional prognostic markers are widely sought. The study aim was to analyze EBV gene expression patterns and LMP1 polymorphism in relation to EBV DNA levels in pediatric liver transplant recipients. EBV load measurement, LMP1 variant, and gene expression analysis were performed in collected prospectively multiple blood samples from 30 patients. Several distinct patterns of EBV gene expression were identified: latency 2 (71%), latency 3 (13%), latency 0 (11%), and lytic infection (5%). In most children's multiple blood samples, both EBV gene expression patterns and expression levels of individual EBV genes varied significantly over time. EBV gene expression patterns were not associated with the EBV load. However, the viral load correlated with the LMP1 and LMP2 expression (r = 0.34; P = 0.006, and r = 0.45; P = 0.001, respectively). Two variants of the LMP1 gene were detected, and they were consistent over time in individual patients. A wild type of LMP1 was associated with higher EBV-DNA loads (P = 0.03). This indicates that EBV infection in immunosuppressed patients is a very dynamic process, but changes in the state of EBV infection do not influence significantly the viral load. The latter, however, can be associated with the activity of LMP1 and LMP2 genes, as well as polymorphism of LMP1.

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Unusually high frequency of a 69-bp deletion within the carboxy terminus of the LMP-1 oncogene of Epstein–Barr virus detected in Burkitt’s lymphoma of Turkish children

Abstract: Burkitt's lymphoma (BL) in

Cited by 21 publications

References 21 publications

Analyses of Epstein‐Barr virus latent membrane protein‐1 in Malaysian nasopharyngeal carcinoma: High prevalence of 30‐bp deletion, Xho1 polymorphism and evidence of dual infections

Analyses of Epstein‐Barr virus latent membrane protein‐1 in Malaysian nasopharyngeal carcinoma: High prevalence of 30‐bp deletion, Xho1 polymorphism and evidence of dual infections

Pediatrik lenfomalarda histopatolojik subtip ve Ebstein-Barr virus ilişkisi: immünohistokimyasal ve in situ hibridizasyon çalışması

Epstein–Barr virus gene expression and latent membrane protein 1 gene polymorphism in pediatric liver transplant recipients

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