Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication

Zhu, Qinghe; Su, Mingjun; Shan, Wei; Shi, Da; Li, Lü; Wang, Jun; Sun, Haibo; Wang, Meijiao; Li, Chunqiu; Guo, Donghua; Sun, Dongbo

doi:10.1016/j.virusres.2022.198916

Cited by 5 publications

(5 citation statements)

References 43 publications

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“…The identification of this RL11_human in this analysis, along with its alignment with the variation profile of the antigen antibody group from the antibody testing, suggests relevance to previous reports [18][19][20]. According to the report by Q. Zhu et al [19], they propose the potential to create a conducive cellular environment that significantly facilitates cell replication and promotes a favorable context for virus replication. They have elucidated that the expression of 60S Ribosomal protein RPL18 notably increases following Covid-19 infection.…”

Section: Results and Discusstionssupporting

confidence: 67%

“…Investigation into this phenomenon revealed previous reports of a similar significant increase in the expression of this 60S Ribosomal protein after Covid-19 infection. Z. Khalid et al have previously reported that 60S Ribosomal protein L29 (RPL29) was highly expressed across all COVID-19 infection groups [18][19][20]. The screened 60S ribosomal protein L11 (RL11_human) in this analysis is a component of the 60S ribosomal subunit, a central part of the ribosome, with several important functions: Ribosome conformation: RL11_human plays a crucial role in the conformation and maturation of ribosomes.…”

Section: Results and Discusstionsmentioning

confidence: 93%

“…The novel coronavirus (Covid- 19), discovered in 2019, demonstrated a formidable infectiousness and rapidly evolved into a global pandemic, spreading across the world and causing a high number of infections and fatalities. Researchers worldwide responded swiftly, focusing on the rapid development of treatments and vaccines.…”

Section: Introductionmentioning

confidence: 99%

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Deep proteome analysis of time-series human plasma samples applied to Covid-19 antibody test

Fukuda,

Nakayama,

Chikaoka

et al. 2023

Preprint

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For the purpose of Covid-19 antibody testing, the human plasma samples acquired over a period of 310 days from August 18, 2021, to June 22, 2022, were subjected to DIA LC-MS proteome analysis. This process led to the acquisition of a quantitative protein profile and allowed for the validation of the temporal behavior of expressed proteins within the samples. A total of 1502 proteins were identified from the plasma samples. Before vaccination, after the first dose, after the second dose, and during the period after contracting the novel coronavirus, protein quantification values during each event interval were compared. Despite minimal changes observed before and after Covid-19 vaccination, notable proteins exhibiting distinct high-expression and low-expression patterns were identified after contracting the novel coronavirus infection.

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Section: Results and Discusstionssupporting

confidence: 67%

Section: Results and Discusstionsmentioning

confidence: 93%

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Deep proteome analysis of time-series human plasma samples applied to Covid-19 antibody test

Fukuda,

Nakayama,

Chikaoka

et al. 2023

Preprint

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“…Since RPS18 protein expression is not different for normal and infected cells it had to be silenced with siRNA to look for its effects 45 but it was found that RPS18 forms an important cluster of NS1 targetting proteins. It was found that DENV infection results in relocation of RPS18 protein to the perinuclear position and indeed it is found to have profound effect in virus replication 47 . Involvement of RPS18 protein in DENV replication can also be suggested by its high amount of presence in nucleus compartment (Fig: 8).…”

Section: Resultmentioning

confidence: 99%

Effect of Human microRNA on Dengue Virus Genome: a cross-specie approach

-¹

2022

IJFMR

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Dengue virus infection being a menace worldwide particularly in West Bengal demanded investigation of a new therapeutic approach. In our work we have performed in silico analysis of target specificity of miRNA to dengue virus genome. Several human miRNAs like hsa-miR-4758-3p_MIMAT0019904, hsa-miR-6760-3p_MIMAT0027421, hsa-miR-6839-3p_MIMAT0027581, hsa-miR-6842-3p_MIMAT0027587 are found to be directly interacting with dengue virus proteins like Envelope, NS1, NS3 and 3’UTR region. The target proteins of respective dengue proteins were analysed using stringDB and Cytoscape. Analysis of the target protein interaction network revealed ACTB, AKT1, TLR4 as few of the hub genes that can be indirectly regulated by miRNA and dengue genome interaction. Thus our work focuses on finding novel miRNA that are not known to regulate dengue infection till date and proposes a novel therapeutic strategy.

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“…Mechanistically, the N protein interacts with p53 in the nucleus via the N S171-N194 motif, maintaining a high level of p53 and activating the p53-DREAM signaling pathway to inhibit the expression of cyclin A, and subsequently inducing S phase arrest [65]. A subsequent proteomic study showed that 60S ribosomal protein L18 (RPL18) was significantly increased in S phase arrested cells induced by N protein expression or PEDV infection [127]. In contrast, a recent study found that the N protein virus bound to constitutively photomorphogenic 1 (COP1) via its N-terminal domain (1-124 aa) and inhibited COP1 degradation, thereby promoting the COP1-mediated ubiquitination and degradation of p53, abolishing the antiviral activity of p53 [66].…”

Section: N Proteinmentioning

confidence: 99%

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

Yu,

Dong,

Chen

et al. 2024

Vaccines

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Porcine epidemic diarrhea virus (PEDV) is the etiology of porcine epidemic diarrhea (PED), a highly contagious digestive disease in pigs and especially in neonatal piglets, in which a mortality rate of up to 100% will be induced. Immunizing pregnant sows remains the most promising and effective strategy for protecting their neonatal offspring from PEDV. Although half a century has passed since its first report in Europe and several prophylactic vaccines (inactivated or live attenuated) have been developed, PED still poses a significant economic concern to the swine industry worldwide. Hence, there is an urgent need for novel vaccines in clinical practice, especially live attenuated vaccines (LAVs) that can induce a strong protective lactogenic immune response in pregnant sows. Reverse genetic techniques provide a robust tool for virological research from the function of viral proteins to the generation of rationally designed vaccines. In this review, after systematically summarizing the research progress on virulence-related viral proteins, we reviewed reverse genetics techniques for PEDV and their application in the development of PED LAVs. Then, we probed into the potential methods for generating safe, effective, and genetically stable PED LAV candidates, aiming to provide new ideas for the rational design of PED LAVs.

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Up-regulated 60S ribosomal protein L18 in PEDV N protein-induced S-phase arrested host cells promotes viral replication

Cited by 5 publications

References 43 publications

Deep proteome analysis of time-series human plasma samples applied to Covid-19 antibody test

Deep proteome analysis of time-series human plasma samples applied to Covid-19 antibody test

Effect of Human microRNA on Dengue Virus Genome: a cross-specie approach

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

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