Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells

Li, Yang; Du, Bin; Pan, Jiaji; Chen, Dechang; Liu, Dawei

doi:10.1631/jzus.2006.b0899

Cited by 5 publications

(4 citation statements)

References 20 publications

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“…Similar increases in IL-8 production have been demonstrated in human umbilical vein endothelial cells and coronary artery endothelial cells exposed to LPS in vitro (26,46). It is likely that the BPMEC cell response to LPS is dependent on the expression of TRL-4 by the endothelial cells.…”

Section: Vol 15 2008 Effects Of Lps and Leukotoxin On Bovine Lung Csupporting

confidence: 70%

Effects of Lipopolysaccharide andMannheimia haemolyticaLeukotoxin on Bovine Lung Microvascular Endothelial Cells and Alveolar Epithelial Cells

McClenahan¹,

Hellenbrand²,

Atapattu³

et al. 2008

Clin Vaccine Immunol

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Bovine respiratory disease resulting from infection with Mannheimia haemolytica commonly results in extensive vascular leakage into the alveoli. M. haemolytica produces two substances, lipopolysaccharide (LPS) and leukotoxin (LKT), that are known to be important in inducing some of the pathological changes. In the present study, we examined bovine pulmonary epithelial (BPE) cell and bovine lung microvascular endothelial cell monolayer permeability, as measured by trans-well endothelial and epithelial cell electrical resistance (TEER), after incubation with LPS, LKT, or LPS-activated neutrophils. Endothelial cell monolayers exposed to LPS exhibited significant decreases in TEER that corresponded with increased levels of proinflammatory cytokines, apoptosis, and morphological changes. In contrast, BPE cells exposed to LPS increased the levels of production of inflammatory cytokines but displayed no changes in TEER, apoptosis, or visible morphological changes. Both cell types appeared to express relatively equal levels of the LPS ligand Toll-like receptor 4. However, TEER in BPE cell monolayers was decreased when the cells were incubated with LPS-activated neutrophils. Although the incubation of BPE cells with LKT decreased TEER, this was not reduced by the incubation of LKT with a neutralizing antibody and was reversed when LKT was preincubated with the LPS-neutralizing compound polymyxin B. Because BPE cells did not express the LKT receptor CD11a/CD18, we infer that contaminating LPS was responsible for the decreased TEER. In conclusion, LPS triggered changes in endothelial cells that would be consistent with vascular leakage, but neither LPS nor LKT caused similar changes in epithelial cells, unless neutrophils were also present.

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Section: Vol 15 2008 Effects Of Lps and Leukotoxin On Bovine Lung Csupporting

confidence: 70%

Effects of Lipopolysaccharide andMannheimia haemolyticaLeukotoxin on Bovine Lung Microvascular Endothelial Cells and Alveolar Epithelial Cells

McClenahan¹,

Hellenbrand²,

Atapattu³

et al. 2008

Clin Vaccine Immunol

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“…In this study, rats in the untreated group had lower IL‐6 concentrations than the drotrecogin treated group, which may indicate that drotrecogin might indeed be deleterious in the early phase of acute pancreatitis. However, Li et al [ 20 showed that on human umblical vein endothelial cells stimulated with lipopolysaccharide, APC increased interleukin‐6 levels. Therefore, the higher interleukin‐6 levels in the APC group in our study may be the direct result of APC administration.…”

Section: Discussionmentioning

confidence: 99%

Use of activated protein C has no avail in the early phase of acute pancreatitis

Akay

Özütemiz

Yenisey

et al. 2008

HPB

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“…17 However, in a study of isolated human monocytes, APC had no effect on IL-8 20 and in endothelial cells APC was reported to up-regulate both IL-6 and IL-8. 23 Thus, there are clear cell-specific differences.…”

Section: Q4mentioning

confidence: 99%

Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions

Galley¹,

Sakka

Webster³

et al. 2008

British Journal of Anaesthesia

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Background. Activated protein C (APC) therapy reduces mortality in high-risk patients with severe sepsis. The effects of APC on inflammatory responses have also been reported. Neutrophils are key cells involved in early host defence mechanisms in sepsis. We hypothesized that APC may have effects on neutrophil function. Methods. Neutrophils were isolated from 10 healthy volunteers and incubated in the presence of lipopolysaccharide (LPS) with and without a range of therapeutically relevant concentrations of recombinant human APC. Respiratory burst activity was determined using flow-activated cell sorting (FACS) analysis. Apoptosis was determined using Annexin-V staining and FACS analysis. Cytokine bead array was used to simultaneously measure three key cytokines in culture supernatants: interleukin (IL)-1b,-6, and-8. For chemotaxis, neutrophil migration through a 5 mm membrane was measured in response to formyl-methyl-leucinephenylalanine (FMLP) or IL-8 in the presence and absence of APC. Results. Exposure to LPS resulted in significant increases in respiratory burst activity, IL-1b,-6, and-8 expression (all P,0.0001) and decreased the number of apoptotic cells (P,0.0001). The APC exposure resulted in a significant release of IL-6 (P¼0.04) without affecting other cytokines. Respiratory burst and apoptosis were also unaffected by APC. Neutrophil chemotaxis in response to either FMLP or IL-8 was reduced by APC (P¼0.005 and 0.007, respectively). Conclusions. This pilot study showed that APC treatment of human neutrophils results in a decreased IL-6 expression and chemotaxis, without affecting other cytokines, apoptosis, or respiratory burst activity.

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Up-regulation interleukin-6 and interleukin-8 by activated protein C in lipopolysaccharide-treated human umbilical vein endothelial cells

Cited by 5 publications

References 20 publications

Effects of Lipopolysaccharide andMannheimia haemolyticaLeukotoxin on Bovine Lung Microvascular Endothelial Cells and Alveolar Epithelial Cells

Effects of Lipopolysaccharide andMannheimia haemolyticaLeukotoxin on Bovine Lung Microvascular Endothelial Cells and Alveolar Epithelial Cells

Use of activated protein C has no avail in the early phase of acute pancreatitis

Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions

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