Up-regulation of Cytoplasmic CD24 Expression Is Associated with Malignant Transformation but Favorable Prognosis of Colorectal Adenocarcinoma

Abstract

(I to III versus IV) (p=0.005, p=0.034, and p=0.006, respectively

Profiling cytochrome P450 family 4 gene expression in human hepatocellular carcinoma

“…Immunohistochemical staining of the tissue sections from the TMA paraffin blocks was performed as previously described (12). A primary rabbit polyclonal antibody against human CYP4F2 (ab111741, diluted 1:100; Abcam, Cambridge, UK) was used; the reactions were incubated at room temperature for 1 h. A modified Allred et al (13) method was applied to evaluate both the intensity of the immunohistochemical staining and the proportion of stained neoplastic or non-neoplastic hepatocytes on each slide.…”

Cytochrome P450 4A11 expression in tumor cells: A favorable prognostic factor for hepatocellular carcinoma patients

“…Immunohistochemical staining of the tissue sections from the tissue microarrays paraffin blocks was performed as previously described . A primary rabbit polyclonal antibody to human CYP4A11, which is homologous to human CYP4A11 (product # PA5‐30004, diluted 1:100; Thermo Fisher Scientific, Rockford, IL, USA), was used; the reactions were incubated at room temperature for 1 h.…”

Stem Cell Markers in Colon Cancer

“…Its expression was confirmed in normal nervous tissue [16] and in cancers of the colon [5], pancreas [24], breast, and prostate [26]. CD24 is involved in cellular signaling processes, in cellular differentiation, and in proliferation and is being considered a significant marker of cancer stem cells (CSCs) [4,16,39]. The mechanism by which CD24 participates in signaling processes seems to be related to mitogen-activated protein kinase (MAPK) and serine/threonine pathway [26].…”

“…CD44 is involved in first steps of carcinogenesis and plays an important role in liver metastasis formation [4,9,[41][42][43]. Yeo et al found CD24 a useful diagnostic marker of early colon cancer [39], whereas its expression was higher in malignant polyps than CD24 expression in colon adenomatous lesions.…”

“…No correlation was found between CD24 expression in colon cancer and tumor type or degree of differentiation (G) [5,44]; other authors have highlighted, however, an inverse relation between CD24 expression and tumor size, poor differentiated cancers, and advanced TNM stages [39]. Regarding lymph node involvement and CD24 expression, as association between high CD24 expression and a larger number of lymph nodes involved was reported in some research papers [45] but not in others [5,24].…”