Up‐regulation of estrogen receptor‐β expression during osteogenic differentiation of human periodontal ligament cells

Tang, Xiaolin; Meng, Hua; Han, Jianmin; Zhang, L.; Zhang, F.

doi:10.1111/j.1600-0765.2007.01031.x

Cited by 22 publications

(17 citation statements)

References 38 publications

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“…As has been reported in other cell types, expression of both ERα and ERβ were observed to increase overtime in osteogenic medium, (Fig. 4A, N = 3, * P <0.01) [37], [40]. After 7d differentiation, staining and quantification of alkaline phosphatase (ALP) activity was performed as an early marker of osteogenic differentiation (Fig.…”

Section: Resultssupporting

confidence: 72%

Estrogen/Estrogen Receptor Alpha Signaling in Mouse Posterofrontal Cranial Suture Fusion

et al. 2009

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BackgroundWhile premature suture fusion, or craniosynostosis, is a relatively common condition, the cause is often unknown. Estrogens are associated with growth plate fusion of endochondral bones. In the following study, we explore the previously unknown significance of estrogen/estrogen receptor signaling in cranial suture biology.Methodology/Principal FindingsFirstly, estrogen receptor (ER) expression was examined in physiologically fusing (posterofrontal) and patent (sagittal) mouse cranial sutures by quantitative RT-PCR. Next, the cranial suture phenotype of ER alpha and ER beta knockout (αERKO, βERKO) mice was studied. Subsequently, mouse suture-derived mesenchymal cells (SMCs) were isolated; the effects of 17-β estradiol or the estrogen antagonist Fulvestrant on gene expression, osteogenic and chondrogenic differentiation were examined in vitro. Finally, in vivo experiments were performed in which Fulvestrant was administered subcutaneously to the mouse calvaria. Results showed that increased ERα but not ERβ transcript abundance temporally coincided with posterofrontal suture fusion. The αERKO but not βERKO mouse exhibited delayed posterofrontal suture fusion. In vitro, addition of 17-β estradiol enhanced both osteogenic and chondrogenic differentiation in suture-derived mesenchymal cells, effects reversible by Fulvestrant. Finally, in vivo application of Fulvestrant significantly diminished calvarial osteogenesis, inhibiting suture fusion.Conclusions/SignificanceEstrogen signaling through ERα but not ERβ is associated with and necessary for normal mouse posterofrontal suture fusion. In vitro studies suggest that estrogens may play a role in osteoblast and/or chondrocyte differentiation within the cranial suture complex.

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Section: Resultssupporting

confidence: 72%

Estrogen/Estrogen Receptor Alpha Signaling in Mouse Posterofrontal Cranial Suture Fusion

et al. 2009

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“…2005). Furthermore, ERβ may play an active part in the osteogenic differentiation of periodontal ligament cells (Tang et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical detection of estrogen receptor β in alveolar bone cells of estradiol‐treated female rats: possible direct action of estrogen on osteoclast life span

2009

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The role of estrogen in bone resorption has been specifically related to the effect of estrogen on the signalling pathway that inhibits the formation of osteoclasts. However, osteoclast apoptosis and a significant reduction in the number of these cells have been observed in the alveolar bone of female rats treated with estradiol. In the present study, the expression of estrogen receptor b (ERb) in the cells of alveolar bone was evaluated in estradiol-treated and -untreated female rats. In order to test the possible direct action of estrogen on osteoclasts, the relationship between apoptosis and ERb expression in these cells was also analysed. The animals received estradiol for 14 days and the alveolar bone fragments were embedded in paraffin for the quantification of tartrate-resistant acid phosphatase-positive osteoclasts. The expression of ERb and apoptosis in the osteoclasts were evaluated by ERb immunohistochemistry and Terminal deoxynucleotidyl transferase-mediated dUTP NickEnd Labelling (TUNEL) methods, respectively. To confirm osteoclast death by apoptosis, these cells were analysed under transmission electron microscopy. Some osteoclasts from estradiol-treated animals were found to be undergoing apoptosis and the number of tartrate-resistant acid phosphatase-positive osteoclasts was significantly reduced. ERb immunolabelling was observed in the cytoplasm and nuclei of active osteoblasts, osteocytes and osteoclasts in both groups, suggesting a direct participation of estrogen on alveolar bone cells. However, following estradiol treatment, a strong ERb immunolabelling was often observed in the TUNEL-positive osteoclasts. Therefore, these results indicate that, in addition to the other signalling pathway, the reduction of alveolar bone resorption is also related to a direct action of estrogen on osteoclasts, promoting apoptosis in these cells, via ERb.

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“…Primary culture of hGF and hPDLC was carried out according to previously described methods [7], [26], [36], [43]. Briefly, hPDLC were prepared from extracted third molars of 5 young healthy volunteers and hGF were obtained from gingiva of the same 5 donors.…”

Section: Methodsmentioning

confidence: 99%

“…The remaining cells were harvested using lysis buffer [20 mM Tris (pH 7.4), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% (v/v) Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerol phosphate and 2 mM Na 3 VO 4 supplemented with protease inhibitor cocktail (Roche, Mannheim, Germany)] [43] for Western blotting. Protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Applygen, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Autocrine/Paracrine Function of Vitamin D in Human Gingival Fibroblasts and Periodontal Ligament Cells

2012

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BackgroundWe previously demonstrated that 25-hydroxyvitamin D3, the precursor of 1α,25-dihydroxyvitamin D3, is abundant around periodontal soft tissues. Here we investigate whether 25-hydroxyvitamin D3 is converted to 1α,25-dihydroxyvitamin D3 in periodontal soft tissue cells and explore the possibility of an autocrine/paracrine function of 1α,25-dihydroxyvitamin D3 in periodontal soft tissue cells.Methodology/Principal FindingsWe established primary cultures of human gingival fibroblasts and human periodontal ligament cells from 5 individual donors. We demonstrated that 1α-hydroxylase was expressed in human gingival fibroblasts and periodontal ligament cells, as was cubilin. After incubation with the 1α-hydroxylase substrate 25-hydroxyvitamin D3, human gingival fibroblasts and periodontal ligament cells generated detectable 1α,25-dihydroxyvitamin D3 that resulted in an up-regulation of CYP24A1 and RANKL mRNA. A specific knockdown of 1α-hydroxylase in human gingival fibroblasts and periodontal ligament cells using siRNA resulted in a significant reduction in both 1α,25-dihydroxyvitamin D3 production and mRNA expression of CYP24A1 and RANKL. The classical renal regulators of 1α-hydroxylase (parathyroid hormone, calcium and 1α,25-dihydroxyvitamin D3) and Porphyromonas gingivalis lipopolysaccharide did not influence 1α-hydroxylase expression significantly, however, interleukin-1β and sodium butyrate strongly induced 1α-hydroxylase expression in human gingival fibroblasts and periodontal ligament cells.Conclusions/SignificanceIn this study, the expression, activity and functionality of 1α-hydroxylase were detected in human gingival fibroblasts and periodontal ligament cells, raising the possibility that vitamin D acts in an autocrine/paracrine manner in these cells.

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Up‐regulation of estrogen receptor‐β expression during osteogenic differentiation of human periodontal ligament cells

Abstract: The results suggest that estrogen receptor-beta may be the predominant subtype expressed in human periodontal ligament cells and may actively participate in the osteogenic differentiation process of human periodontal ligament cells, both in male and in female subjects.

Cited by 22 publications

References 38 publications

Estrogen/Estrogen Receptor Alpha Signaling in Mouse Posterofrontal Cranial Suture Fusion

Estrogen/Estrogen Receptor Alpha Signaling in Mouse Posterofrontal Cranial Suture Fusion

Immunohistochemical detection of estrogen receptor β in alveolar bone cells of estradiol‐treated female rats: possible direct action of estrogen on osteoclast life span

Characterization of the Autocrine/Paracrine Function of Vitamin D in Human Gingival Fibroblasts and Periodontal Ligament Cells

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