Up regulation of KAI1 gene expression and apoptosis effect of imatinib mesylate in gastric adenocarcinoma (AGS) cell line

Shandiz, Seyed Ataollah Sadat; Farasati, Soheila; Saeedi, Banafshe; Baghbani-Arani, Fahimeh; Asl, Elham Akbari; Keshavarz-Pakseresht, Behta; Rahimi, Arian; Assadi, Artin; Noorbazargan, Hassan; Hesari, Maryam Rahimpour; Mirzaie, Amir

doi:10.1016/s2222-1808(15)60996-3

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…To determine apoptosis and necrosis in HT29 cell line treated with IC 50 concentrations of AgNPs, the cells were stained with FITC Annexin V and PI and studied by flow cytometry (Figure 11) . During the early phase of apoptosis, phosphatidylinositol serine is translocate to cell membrane and is stained by annexin V, whereas, PI binds to nucleus in necrotic cells [39]. Flow cytometry results are shown in Figure 3, in which the upper left square (Q1) represents the percentage of late apoptotic cells and top left square (Q2) early apoptotic cells.…”

Section: Apoptosis and Necrosis Testmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Dehghanizade

Arasteh

Mirzaie

2017

Artificial Cells, Nanomedicine, and Biotechnology

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This study aim to synthesize silver nanoparticles (AgNPs) using Anthemis atropatana extract and to evaluate their chemical characteristics and antimicrobial and cytotoxic effects. The biosynthesis of AgNPs is verified using UV-visible spectrum which showing maximum absorption in 430 nm wavelength. Transmission electron microscope (TEM) and scanning electron microscope (SEM) results revealed that AgNPs has a spherical shape with an average size of 38.89 nanometres. The crystalline structure of green synthesized AgNPs in optimal conditions was confirmed by XRD analysis. The pattern of XRD peaks related to face-centred cubic (fcc) (111), (200), (220), (311) and (222) observed. Also, FTIR results verified the AgNPs synthesis using plant extract. In biological tests, the MTT results indicate the dose dependence of cytotoxic effects of AgNPs on colon cancer cell lines (HT29). The AgNPs had maximum cytotoxicity on HT29 cancer cell line at 100 μg/ml concentration, which were statistically significant comparing control cells (p < .001). Moreover, real time PCR and flow cytometry results confirmed the apoptotic effects of AgNPs. According to the results, it seems that the green synthesis of AgNPs is an eco-friendly and cost effective approach. This research provides insight into the development of new anticancer and antibacterial agents.

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Section: Apoptosis and Necrosis Testmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Dehghanizade

Arasteh

Mirzaie

2017

Artificial Cells, Nanomedicine, and Biotechnology

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“…Annexin V stains PS of the early and late apoptotic cells, while PI detects the nucleus of the cells, which have a disrupted integrity due to necrosis. 45 Figure 10 shows that …”

Section: Analysis Of Apoptosis-related Gene Expressionmentioning

confidence: 99%

Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

Salehi

Shandiz

Ghanbar

et al. 2016

IJN

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A rapid phytosynthesis of silver nanoparticles (AgNPs) using an extract from the aerial parts of Artemisia marschalliana Sprengel was investigated in this study. The synthesized AgNPs using A. marschalliana extract was analyzed by UV–visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. Characteristic absorption bands of AgNPs were found near 430 nm in the UV–vis spectrum. Energy-dispersive spectroscopy analysis of AgNPs in the energy range 2–4 keV confirmed the silver signal due to surface plasmon resonance. Scanning electron microscopy and transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 5 nm to 50 nm. The zeta potential value of −31 mV confirmed the stability of the AgNPs. AgNPs produced using the aqueous A. marschalliana extract might serve as a potent in vitro antioxidant, as revealed by 2,2-diphenyl-1-picryl hydrazyl assay. The present study demonstrates the anticancer properties of phytosynthesized AgNPs against human gastric carcinoma AGS cells. AgNPs exerted a dose-dependent inhibitory effect on the viability of cells. Real-time polymerase chain reaction was used for the investigation of Bax and Bcl-2 gene expression in cancer and normal cell lines. Our findings show that the mRNA levels of pro-apoptotic Bax gene expression were significantly upregulated, while the expression of anti-apoptotic Bcl-2 was declined in cells treated with AgNPs compared to normal cells. In addition, flow cytometric analysis showed that the number of early and late apoptotic AGS cells was significantly enhanced following treatment with AgNPs as compared to untreated cells. In addition, the AgNPs showed strong antibacterial properties against tested pathogenic bacteria such as Staphylococcus aureus , Bacillus cereus , Acinetobacter baumannii , and Pseudomonas aeruginosa . Based on the obtained data, we suggest that phytosynthesized AgNPs are good alternatives in the treatment of diseases because of the presence of bioactive agents.

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“…15 IM has been shown to compete with adenosine triphosphate and inhibit specific tyrosine kinases such as Bcr-Abl kinase, c-kit receptor kinases activation, as well as inhibit platelet-derived growth factor (a, b) receptor. 16 Several studies were designed to explore the growth inhibitory effect of IM either in vitro (cancer cell lines) or in vivo (tumor-bearing experimental animals) on variety of cancer cell types, such as of breast, 17,18 liver, 19 prostate, 20 and colorectal origin 21 as well as many other types of malignant tumors. 22,23 Besides IM great effectiveness, patients treated with IM may suffer from its side effects and long-term toxicity as renal, cardiac, and hepatotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Maximizing the Therapeutic Efficacy of Imatinib Mesylate–Loaded Niosomes on Human Colon Adenocarcinoma Using Box-Behnken Design

Kassem

El-Sawy

Abd-Allah

et al. 2017

Journal of Pharmaceutical Sciences

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This research purposed to formulate an optimized imatinib mesylate (IM)-loaded niosomes to improve its chemotherapeutic efficacy. The influence of 3 formulation factors on niosomal vesicular size (Y), zeta potential (Y), entrapment capacity percentage (Y), the percentage of initial drug release after 2 h (Y), and the percentage of cumulative drug release after 24 h (Y) were studied and optimized using Box-Behnken design. Optimum desirability was specified and the optimized formula was prepared, stability tested, morphologically examined, checked for vesicular bilayer formation and evaluated for its in vitro cytotoxicity on 3 different cancer cell lines namely MCF-7, HCT-116, and HepG-2 in addition to 1 normal cell line to ensure its selectivity against cancer cells. The actual responses of the optimized IM formulation were 425.36 nm, -62.4 mV, 82.96%, 18.93%, and 89.45% for Y, Y, Y, Y, and Y, respectively. The optimized IM-loaded niosomes confirmed the spherical vesicular shape imaged by both light and electron microscopes and further proven by differential scanning calorimetry. Moreover, the optimized formula exhibited improved stability on storage at 4 ± 2°C and superior efficacy on MCF7, HCT-116, and HepG2 as IC values were 6.7, 16.4, and 7.3 folds less than those of free drug, respectively. Interestingly, IC of the optimized formula against normal cell line was ranged from 3 to 11 folds higher than in different cancer cells indicating a higher selectivity of the optimized formula to cancer cells. In conclusion, the incorporation of IM in niosomes enhanced its efficacy and selectivity toward cancer cells, presenting a promising tool to fight cancer using this approach.

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Up regulation of KAI1 gene expression and apoptosis effect of imatinib mesylate in gastric adenocarcinoma (AGS) cell line

Cited by 10 publications

References 34 publications

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

Maximizing the Therapeutic Efficacy of Imatinib Mesylate–Loaded Niosomes on Human Colon Adenocarcinoma Using Box-Behnken Design

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