Green synthesis of silver nanoparticles using Anthemis atropatana extract: characterization and in vitro biological activities

Abstract

This study aim to synthesize silver nanoparticles (AgNPs) using Anthemis atropatana extract and to evaluate their chemical characteristics and antimicrobial and cytotoxic effects. The biosynthesis of AgNPs is verified using UV-visible spectrum which showing maximum absorption in 430 nm wavelength. Transmission electron microscope (TEM) and scanning electron microscope (SEM) results revealed that AgNPs has a spherical shape with an average size of 38.89 nanometres. The crystalline structure of green synthesized AgNPs in optimal conditions was confirmed by XRD analysis. The pattern of XRD peaks related to face-centred cubic (fcc) (111), (200), (220), (311) and (222) observed. Also, FTIR results verified the AgNPs synthesis using plant extract. In biological tests, the MTT results indicate the dose dependence of cytotoxic effects of AgNPs on colon cancer cell lines (HT29). The AgNPs had maximum cytotoxicity on HT29 cancer cell line at 100 μg/ml concentration, which were statistically significant comparing control cells (p < .001). Moreover, real time PCR and flow cytometry results confirmed the apoptotic effects of AgNPs. According to the results, it seems that the green synthesis of AgNPs is an eco-friendly and cost effective approach. This research provides insight into the development of new anticancer and antibacterial agents.

Augmented photocatalytic, antibacterial and antifungal activity of prunosynthetic silver nanoparticles

“…PCFE phytochemicals reduced and stabilized the DIBSNPs and SLEBSNPs maximally at longer incubation period of 150 min. The augmented absorbance and intense dark brown colour turning to grey indicated the formation of greater [15,16]. Both the reactions in dark as well in sunlight exposed generated 442 and 410 nm absorption maxima respectively thus pointing towards the possibility of prunosynthesis of BSNPs in sunlight where no additional magnetic stirring and heat were provided as in case of DIBSNPs.…”

Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract

“…These nanostructures interact with cell membrane, proteins and nucleic acids inside the bacteria by their large surface area as unique property [21]. In recent years, many investigations have proved beneficial aspects of NPS synthesis through using plant extracts as green biochemistry method [55]. Therefore, we reported the green synthesis of Ag, Cu and TiO 2 NPs by aqueous leaf extract of A. haussknechtii.…”

Multifunctional fluorescent titania nanoparticles: green preparation and applications as antibacterial and cancer theranostic agents

“…The antibacterial activity of the samples (FTN and HTN) with different concentrations (0-1000 lg/mL) was evaluated against gram-negative E. coli ATCC 25922 and gram-positive S. aureus ATCC 25923 strains regarding the minimum inhibitory concentration (MIC) using a broth microdilution method followed by the CLSI guidelines [11]. MIC, defined as the lowest concentration of the nanoparticles, inhibits the growth of the bacteria with no significant increases [12]. In general, 10 lL of each bacterial suspension (10 8 CFU/mL) was added to each well containing 100 lL growth medium with varying nanoparticles concentration.…”