Up-regulation of microRNA-335-5p reduces inflammation via negative regulation of the TPX2-mediated AKT/GSK3β signaling pathway in a chronic rhinosinusitis mouse model

Gu, Xiaoqing; Yao, Xiaocui; Liu, Dengtao

doi:10.1016/j.cellsig.2020.109596

Cited by 27 publications

(26 citation statements)

References 38 publications

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“…The importance of miRNA regulation in the pathogenesis of the aforementioned allergic diseases is supported by many studies, but also in other allergic diseases such as food allergy or chronic rhinosinusitis the evidence for a role of miRNAs is emerging. 4,10,[13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Figure 2 provides an overview of miRNAs in cells and tissues that are associated with allergic diseases presented herein.…”

Section: Introductionmentioning

confidence: 99%

Spotlight on microRNAs in allergy and asthma

Weidner

Bartel

Kılıç

et al. 2020

Allergy

113

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Section: Introductionmentioning

confidence: 99%

Spotlight on microRNAs in allergy and asthma

Weidner

Bartel

Kılıç

et al. 2020

Allergy

113

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“…The miRNAs miR3074-1-3p, miR3095-3p, miR344b-3p, and miR3057-5p have no reported biological functions. For the decreased miRNAs, miR495-3p [58], miR140-3p [59], miR434-5p [60], miR322-3p [61], miR409-3p [62], miR674-3p [63], miR335-3p [61], miR543-3p [64], miR341-3p [65], miR202-5p [66], miR369-3p [67], miR330-3p [68], miR370-3p [69], miR335-5p [70], miR503-3p [71], and miR503-5p [72] have been reported that related to biological processes, and only miR299a-3p has no reported function. We veri ed the 18 increased miRNAs by qPCR, and Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Jiang¹,

Ma²,

Han³

et al. 2021

Preprint

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The cochlea is an important sensory organ for both balance and sound perception, and the formation of the cochlea is a complex developmental process. The development of the mouse cochlea begins on embryonic day (E)9 and continues until postnatal day (P)21 when the hearing system is considered mature. Small extracellular vesicles (sEVs), with a diameter ranging from 30 nm to 200 nm, have been considered as a significant medium for information communication in both the processing of physiological and pathological. However, there are no studies exploring the role of sEVs in the development of the cochlea. Here, we isolated tissue-derived sEVs from the cochleae of FVB mice at P3, P7, P14, and P21 by ultracentrifugation. These sEVs were first characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Next, we used small RNA-seq and mass spectrometry to characterize the microRNA transcriptomes and proteomics of cochlear sEVs from mice at different ages. Many microRNAs and proteins were discovered to be related with inner ear development, anatomical structure development, and the auditory nervous system development. These results all suggest that sEVs exist in the cochlea and are likely to be essential for the normal development of the auditory system. Our findings provide many sEV microRNA and protein targets for future studies of the roles of cochlear sEVs.

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“…In ovarian cancer, it can promote the proliferation and migration of human ovarian cancer cells by regulating PLK1 expression [40]. Except for these function, in various cancers can TPX2 also control bladder cancer cell's proliferation and invasion via TPX2-p53-GLIPR1 regulatory circuitry [41], regulate the PI3K/AKT signaling pathway to facilitate hepatocellular carcinoma [42], interactive with miRNA such as miR-485-3p [43], miR-361-5p [44], miR-335-5p [45], miR-216b [46] and so on. Zhou et al have veri ed that TPX2 can activate the epithelial-mesenchymal transition process and promote both the expression and activities of matrix metalloproteinase (MMP)2 and MMP9 in non-small cell lung cancer (NSCLC), which means TPX2 promotes the metastasis and malignant progression of NSCLC and could thus serve as a marker of poor prognosis in NSCLC [47].…”

Section: Discussionmentioning

confidence: 99%

Identification of molecular markers associated with diagnosis and prognosis in lung adenocarcinoma by bioinformatics analysis

Wei

Zang

Zhao

2021

Preprint

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Background: Lung adenocarcinoma (LUAD) is the main histological subtype of lung cancer. However, the molecular mechanism underlying LUAD is not yet clearly defined, but elucidating this process in detail would be of great significance for clinical diagnosis and treatment.Methods: Gene expression profiles were retrieved from Gene Expression Omnibus database (GEO), and the common differentially expressed genes (DEGs) were identified by online GEO2R analysis tool. Subsequently, the enrichment analysis of function and signaling pathways of DEGs in LUAD were performed by gene ontology (GO) and The Kyoto Encyclopedia of Genes and Genomics (KEGG) analysis. The protein-protein interaction (PPI) networks of the DEGs were established through the Search Tool for the Retrieval of Interacting Genes (STRING) database and hub genes were screened by plug-in CytoHubba in Cytoscape. Afterwards, the miRNAs and the hub genes network was constructed via miRWalk. Finally, receiver operating characteristic (ROC) curve and Kaplan-Meier plotter were performed to analyze the diagnosis and prognosis efficacy of hub genes.Results: A total of 312 DEGs were identified, including 74 up-regulated and 238 down-regulated genes. GO analysis results showed that DEGs were mainly enriched in biological processes including composition of extracellular matrix, regulation of angiogenesis and so on. KEGG analysis results revealed DEGs were mainly enrolled in cell adhesion signaling pathway. Subsequently, 10 hub genes, CDC20, CENPF, TPX2, TOP2A, KIAA0101, CDCA7, ASPM, ECT2, UBE2T and COL1A1, were identified. And TOP2A, CDCA7, TPX2 and COL1A1 showed strong relationships with each other and the miRNAs nearby in miRNAs-mRNA network obtained by miRWalk website. Finally, all these 10 hub genes were found significantly related to the diagnosis and prognosis of LUAD (p<0.05). Conclusions: Our results suggested that TOP2A, CDCA7, TPX2 and COL1A1 might present predictive value for the development and prognosis in LUAD, and might be used as potential molecular markers for the diagnosis and treatment of LUAD.

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Up-regulation of microRNA-335-5p reduces inflammation via negative regulation of the TPX2-mediated AKT/GSK3β signaling pathway in a chronic rhinosinusitis mouse model

Cited by 27 publications

References 38 publications

Spotlight on microRNAs in allergy and asthma

Spotlight on microRNAs in allergy and asthma

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Identification of molecular markers associated with diagnosis and prognosis in lung adenocarcinoma by bioinformatics analysis

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