Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood

Pastucha, Matěj; Odstrčilíková, Eliška; Hlaváček, Antonín; Brandmeier, Julian C.; Vykoukal, Vít; Weisová, Julie; Gorris, Hans H.; Skládal, Petr

doi:10.1109/jstqe.2021.3049689

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…The suspension was purged for 40 min with argon and then dialyzed in a Float-A-Lyzer G2 dialysis device (100 kDa M W cut-off) against 4 L of dialysis buffer (50 × 10 −3 m Tris, 0.05% NaN 3 , 1 × 10 −3 m KF, pH 7.5 at 4°C for 72 h), which was exchanged nine times. [32]…”

Section: Sa-peg-ucnp Conjugatesmentioning

confidence: 99%

Effect of Particle Size and Surface Chemistry of Photon‐Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Brandmeier

Raiko

Peltomaa

et al. 2021

Adv Healthcare Materials

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Sensitive immunoassays are required for troponin, a low-abundance cardiac biomarker in blood. In contrast to conventional (analog) assays that measure the integrated signal of thousands of molecules, digital assays are based on counting individual biomarker molecules. Photon-upconversion nanoparticles (UCNP) are an excellent nanomaterial for labeling and detecting single biomarker molecules because their unique anti-Stokes emission avoids optical interference, and single nanoparticles can be reliably distinguished from the background signal. Here, the effect of the surface architecture and size of UCNP labels on the performance of upconversion-linked immunosorbent assays (ULISA) is critically assessed. The size, brightness, and surface architecture of UCNP labels are more important for measuring low troponin concentrations in human plasma than changing from an analog to a digital detection mode. Both detection modes result approximately in the same assay sensitivity, reaching a limit of detection (LOD) of 10 pg mL −1 in plasma, which is in the range of troponin concentrations found in the blood of healthy individuals.

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Section: Sa-peg-ucnp Conjugatesmentioning

confidence: 99%

Effect of Particle Size and Surface Chemistry of Photon‐Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Brandmeier

Raiko

Peltomaa

et al. 2021

Adv Healthcare Materials

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“…To further assess the performance of the UCNP-based dot-blot, we compared the results with MTP-based assays (Table and Figure S10), which were carried out according to our previously published protocols. ,, The achieved LODs in the real samples were 125 pg/mL of HSA in 25% urine, 2.5 pg/mL of PSA in 50% serum, and 39 pg/mL of cTn in 25% serum. In all cases, the dot-blot assays resulted in LODs that were 1 order of magnitude higher as compared with MTP assays.…”

Section: Resultsmentioning

confidence: 99%

Upconversion Nanoparticle-Based Dot-Blot Immunoassay for Quantitative Biomarker Detection

Máčala,

Makhneva,

Hlaváček

et al. 2024

Anal. Chem.

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“…It faces intrinsic shortages of low sensitivity and temporary readout. [ 38 ] Fortunately, UCNPs with high photostability and free background can well overcome these problems. ULISA, short for upconversion‐linked immunosorbent assay, has been developed by replacing enzymes with UCNP phosphors in the labeling process.…”

Section: Strategies For Upconversion Nanoparticle‐based Bacteria Dete...mentioning

confidence: 99%

“…The ULISA showed low cross‐reactivity and achieved LOD of 2.9 × 10 3 CFU mL −1 , a 22‐fold improvement compared to the ELISA performed under the same conditions. [ 38 ] It follows that the sensitivity of ULISA is ≈1–3 orders of magnitude higher than that of ELISA, representing more potential in early diagnosis of bacterial infection. In addition, the absorbance should be immediately recorded after stopping the enzymatic reaction in an ELISA protocol, while the luminescent readout in ULISA is stable and can be measured without time constraint.…”

Section: Strategies For Upconversion Nanoparticle‐based Bacteria Dete...mentioning

confidence: 99%

Lanthanide Upconversion Nanoplatforms for Advanced Bacteria‐Targeted Detection and Therapy

et al. 2023

Advanced Optical Materials

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The growing crisis of antibiotic resistance calls for advanced and sustainable antibacterial strategies. In the past few years, lanthanide‐doped upconversion nanoparticles (UCNPs) with unique photophysical properties have been innovatively explored for bacterial detection and therapy, and hold promise for alleviating challenges faced in the post‐antibiotic era. In this review, the interface engineering for the bacteria‐targeted UCNP nanoplatforms is highlighted, the principles and merits of various upconversion detection strategies are discussed, and the latest progress in UCNP‐based antibacterial applications are summarized, especially in deep‐tissue infection imaging and therapy. Finally, the remaining concerns and future efforts for practical applications are put forward. This review is expected to provide comprehensive guidance for diagnosing and combating bacterial infections based on advanced UCNP nanoplatforms.

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Upconversion-Linked Immunoassay for the Diagnosis of Honeybee Disease American Foulbrood

Cited by 8 publications

References 42 publications

Effect of Particle Size and Surface Chemistry of Photon‐Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Effect of Particle Size and Surface Chemistry of Photon‐Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin

Upconversion Nanoparticle-Based Dot-Blot Immunoassay for Quantitative Biomarker Detection

Lanthanide Upconversion Nanoplatforms for Advanced Bacteria‐Targeted Detection and Therapy

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