Upconversion nanoparticles for differential imaging of plant cells and detection of fluorescent dyes

Wu, Xiaofeng; Hu, Pan; Hu, Shigang; Chen, Zenghui; Yan, Huanyuan; Tang, Zhijun; Xi, Zengzhe; Yu, Yi; Dai, Gangtao; Liu, Yunxin

doi:10.1016/s1002-0721(16)60016-9

Cited by 20 publications

(11 citation statements)

References 26 publications

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“…Lanthanide-doped rare-earth upconversion is a popular research eld because nanocrystals exhibit unique properties; they can convert low-energy near-infrared (NIR) light to highenergy visible light, [1][2][3][4][5] particularly when the nanocrystals are doped with sensitizers and activators. The sensitizers can absorb the NIR light, and the energy is transferred to the activators.…”

Section: Introductionmentioning

confidence: 99%

Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery

et al. 2020

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“…In comparison to Er 3+ ions, Yb 3+ ions present higher activity when they play a role of nucleation agent which usually leads to production of NaGdF 4 particles with well crystallinity and large particle size. From HRTEM image (Figure c), nanocrystalline was normative hexagonal crystal facets and excellent crystallinity, and the distance between two neighboring lattice fringes were 0.518 nm along (100) orientation …”

Section: Resultsmentioning

confidence: 99%

“…Core/shell upconversion nanoparticles (Figure 4) exhibit three distinct emission bands which centered at 535 and 655 nm originating from the population of the levels 4 S 3/2 , and 4 F 9/2 down to state 4 I 15/2 , respectively. The whole emission exhibits a dazzling green color to the naked eye …”

Section: Resultsmentioning

confidence: 99%

“…The unique optical properties of upconversion nanoparticles (UCNP) attracted much attention, which could convert low‐energy photons into high‐energy photons . Because of sharp emission, low injury penetrates the skin and zero self‐background fluorescence multicolor imaging, upconversion nanoparticles are expected to quickly move into clinical practice to solve many disease problems Here, we demonstrate a NR‐modified core/shell UCNPs probe for efficient detection of nitrite in plant cells with a test limit of 0.1 µg mL −1 .…”

Section: Introductionmentioning

confidence: 99%

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Dye‐Sensitized Core/Shell Upconversion Nanoparticles for Detecting Nitrites in Plant Cells

Zhan

et al. 2019

Part & Part Syst Charact

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A fluorescent nanoprobe is reported for rapid detection of nitrites (NO2−) in plant cells. The probe is fabricated by linking neutral reds (NR) to the surface of upconversion fluorescent core/shell nanocrystalline with the bridging of polyethylene glycol (PEG) molecules. The fluorescence of upconversion nanoparticles (UCNPs) is stored by NR through fluorescence resonance energy transfer (FRET) under 980 nm excitation that can be released by further linking to NO2−. It is observed that the intensity rate of green to red emission of NR‐modified UCNPs changes linearly with increasing the amount of NO2−. So that concentration of NO2− can be accordingly addressed. Worth mentioning is that, comparing with bare core upconversion nanoparticles (NPs), core/shell UCNPs can greatly reduce the surface quenching of the fluorescence induced by solvents instead of NR and thus leading to the enhancement of signal‐to‐noise ratios. Moreover, excitation of core/shell UCNPs requires only a much lower power (0.06 W cm−2) than bare cores which is beneficial to reducing the decomposition of NR to stabilize the FRET processes. Under the optimum conditions, the detection limit of nitrite in plant cells was 0.1 µg mL−1.

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“…In this study, the distance between the upconversion nanorods and the fiber surface is less than 10 nm (Figure 1d). [37] Previous studies show that the PL intensity has a linear relationship with increasing RhB in the high concentration range (0.8-2 × 10 −9 m). Figure 3b illustrates the setup for the fluorescence measurement of a single water droplet (10 µL) on the nanofiber membranes.…”

Section: Wwwadvopticalmatdementioning

confidence: 92%

Ultrasensitive and Recyclable Upconversion‐Fluorescence Fibrous Indicator Paper with Plasmonic Nanostructures for Single Droplet Detection

Zhang

et al. 2019

Advanced Optical Materials

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attention due to their extensive biological and medical applications. [1][2][3][4][5] Conventional indicator paper such as pH paper is disposable after use. Similarly, a traditional particulate-based fluorescence sensor is also not reusable because it usually suspends fluorescent nanoparticles such as quantum dots and upconversion nanocrystals in the target solution to initiate detection, [6][7][8][9] which are often unstable and irreproducible when contaminated by the target solution. Additionally, these indicator papers and fluorescence sensors usually need large amounts of the target solutions, which severely restricts their widespread application, especially for small amounts of target solutions, such as a single droplet. On the other hand, the sensitivities of current fluorescence indicator paper and sensors are still expected to improve because a higher sensitivity can respond to target solutions with a lower concentration, which will benefit the application of trace detection. [10,11] Therefore, it is highly desired to develop fluorescence indicator paper or sensor that simultaneously possesses excellent sensitivity and recyclability for single droplet detection.Among fluorescent nanoparticles, upconversion nanocrystals, compared with quantum dots, are attractive candidates because they can be excited by near-infrared light, which has a high penetration depth in biological tissues. [12,13] Moreover, fluorophores with stronger fluorescence can suppress background noise and autofluorescence, bringing about more sensitive detection. [14,15] To achieve stronger fluorescence, plasmonic nanostructures are good choices because they can produce strong localized electric fields, affecting excitation or emission process of nearby fluorophores to enhance their fluorescence; this process must not change the chemical construction of fluorophores, which is not an easy task. [16,17] Superhydrophobicity signifies that the material does not readily become damp, with a water contact angle over 150°. [18] To achieve recyclability, immediate contact of fluorescent nanoparticles with target solutions must be avoided. Utilizing electrospinning is a good method to achieve this, because it can easily incorporate nanoparticles into superhydrophobic nanofibers during the electrospinning process, [19][20][21][22] which endows them with a self-cleaning property. In addition, the large specific surface area of electrospun Conventional indicator paper is disposable after use and requires a large amount of target solution. Herein, recyclable upconversion fluorescence indicator papers consisting of upconversion nanorods and plasmonic nanostructures are fabricated by electrospinning. This recyclable indicator paper exhibits good flexibility and superhydrophobicity with enhanced upconversion fluorescence. Rhodamine B (RhB) is selected as the target solution because it is prohibited as a food additive but often occurs in food, and conventional detection methods usually need a large volume of solution (>1 mL) to refine to higher concentratio...

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Upconversion nanoparticles for differential imaging of plant cells and detection of fluorescent dyes

Cited by 20 publications

References 26 publications

Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery

Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery

Dye‐Sensitized Core/Shell Upconversion Nanoparticles for Detecting Nitrites in Plant Cells

Ultrasensitive and Recyclable Upconversion‐Fluorescence Fibrous Indicator Paper with Plasmonic Nanostructures for Single Droplet Detection

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