Update on the status of metrology for metalloproteins

Swart, Claudia; Jakubowski, Norbert

doi:10.1039/c6ja00181e

Cited by 9 publications

(2 citation statements)

References 55 publications

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“…Clinical testing often involves quantification of specific proteins that serve as disease markers, but reliable, reproducible and traceable measurements are currently lacking. Metrology of metalloproteins recognized ICP-MS elemental measurement combined with separation techniques as a valid methodology with traceability due to availability of SRMs [133]. Metalloproteins provide a direct link between elemental metabolomics and proteomics and we expect that increasing number of metabolite measurements will be done by direct measurement of elements.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Elemental metabolomics

2017

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Elemental metabolomics is quantification and characterization of total concentration of chemical elements in biological samples and monitoring of their changes. Recent advances in inductively coupled plasma mass spectrometry have enabled simultaneous measurement of concentrations of > 70 elements in biological samples. In living organisms, elements interact and compete with each other for absorption and molecular interactions. They also interact with proteins and nucleotide sequences. These interactions modulate enzymatic activities and are critical for many molecular and cellular functions. Testing for concentration of > 40 elements in blood, other bodily fluids and tissues is now in routine use in advanced medical laboratories. In this article, we define the basic concepts of elemental metabolomics, summarize standards and workflows, and propose minimum information for reporting the results of an elemental metabolomics experiment. Major statistical and informatics tools for elemental metabolomics are reviewed, and examples of applications are discussed. Elemental metabolomics is emerging as an important new technology with applications in medical diagnostics, nutrition, agriculture, food science, environmental science and multiplicity of other areas.

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Section: Conclusion and Discussionmentioning

confidence: 99%

Elemental metabolomics

2017

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“…Quantitation of AB and MeHg was achieved using a 13 An overview, from a metrological standpoint, of the efforts during the last three years to achieve reliable quantification of metalloproteins has also been published this year 25 . The analytes covered in the review, which include Haemoglobin (HGB), transferrin (TRF), superoxide dismutase (SOD), selenoprotiens and ceruloplasmin, were chosen due to their importance in clinical diagnostics.…”

Section: Crms and Metrologymentioning

confidence: 99%

Atomic Spectrometry Update: review of advances in elemental speciation

Clough

Harrington

Hill

et al. 2018

J. Anal. At. Spectrom.

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This is the ninth Atomic Spectrometry Update (ASU) to focus on advances in elemental speciation and covers a period of approximately 12 months from December 2015. This ASU review deals with all aspects of the analytical atomic spectrometry speciation methods developed for: the determination of oxidation states; organometallic compounds; coordination compounds; metal and heteroatom-containing biomolecules, including metalloproteins, proteins, peptides and amino acids; and the use of metal-tagging to facilitate detection via atomic spectrometry. The review does not cover fractionation, which is sometimes termed operationally defined speciation. As with all ASU reviews the focus of the research reviewed includes those methods that incorporate atomic spectrometry as the measurement technique. However, because speciation analysis is inherently focused on the relationship between the metal(loid) atom and the organic moiety it is bound to, or incorporated within, atomic spectrometry alone cannot be the sole analytical approach of interest. For this reason molecular detection techniques are also included where they have provided a complementary approach to speciation analysis. As in previous years, As and Se speciation continues to dominate the current literature, with reports of the speciation of Cr and Hg also increasing, and there are an ever rising number of publications concerning the analysis of 'biomolecules'. Whilst most of this work is still the preserve of the research field some methods are now approaching the robustness and rapidity for use in the clinical setting.

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Biological Chemistry of Toxic Metals and Metalloids, Such as Arsenic, Cadmium, and Mercury

Sarpong‐Kumankomah

Miller

Gailer

2020

Encyclopedia of Analytical Chemistry

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Pollution is the largest environmental cause of disease and premature death in the world which causes an estimated nine million annual premature deaths. Owing to the exceptional longevity of highly toxic arsenic, cadmium, and mercury species in the environment, the Agency for Toxic Substances and Disease Registry (ASTDR) has ranked them within the top 7 chemicals of concern in terms of their frequency, toxicity, and potential for human exposure at national priority sites. Accordingly, the chronic exposure of human populations – including children – to these pollutants via the diet, drinking water and air is of increasing public health concern as it may be causally linked to human diseases, which have no known etiology. To unravel potential toxic metal exposure‐disease relationships, the elucidation of their bioinorganic chemistry in the bloodstream is critical as these processes determine which and how much of an absorbed toxic metal species and/or its metabolites will reach toxicological target organs. Considering the analytical complexity of this biological fluid, appropriate analytical techniques must be employed. We highlight advanced instrumental analytical approaches that can be used and provide examples of how their application has revealed new insight into the mechanisms of chronic toxicity of arsenic, cadmium and mercury species in mammals. A comprehensive understanding of the bioinorganic chemistry of individual toxic metal species is urgently needed to reassess current environmental regulations to reduce the global disease burden.

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Update on the status of metrology for metalloproteins

Abstract: Metalloproteins, which represent about 30% of the total proteome, are often important markers for distinguishing between healthy and diseased states of patients.

Cited by 9 publications

References 55 publications

Elemental metabolomics

Elemental metabolomics

Atomic Spectrometry Update: review of advances in elemental speciation

Biological Chemistry of Toxic Metals and Metalloids, Such as Arsenic, Cadmium, and Mercury

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