Updates on the pathogenicity status of Pseudomonas aeruginosa

Azam, Mohammad; Khan, Asad U.

doi:10.1016/j.drudis.2018.07.003

Cited by 270 publications

(199 citation statements)

References 95 publications

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“…This opportunistic pathogen is often a cause of nosocomial infections in immuno-compromised patients. In cystic fibrosis patients P. aeruginosa chronically colonizes the lungs and is a major mortality factor [1,2].…”

Section: Introductionmentioning

confidence: 99%

Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element

et al. 2020

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Background: Pseudomonas aeruginosa is a cause of nosocomial infections, especially in patients with cystic fibrosis and burn wounds. PAO1 strain and its derivatives are widely used to study the biology of this bacterium, however recent studies demonstrated differences in the genomes and phenotypes of derivatives from different laboratories. Results: Here we report the genome sequence of P. aeruginosa PAO1161 laboratory strain, a leu-, Rif R , restrictionmodification defective PAO1 derivative, described as the host of IncP-8 plasmid FP2, conferring the resistance to mercury. Comparison of PAO1161 genome with PAO1-UW sequence revealed lack of an inversion of a large genome segment between rRNA operons and 100 nucleotide polymorphisms, short insertions and deletions. These included a change in leuA, resulting in E108K substitution, which caused leucine auxotrophy and a mutation in rpoB, likely responsible for the rifampicin resistance. Nonsense mutations were detected in PA2735 and PA1939 encoding a DNA methyltransferase and a putative OLD family endonuclease, respectively. Analysis of revertants in these two genes showed that PA2735 is a component of a restriction-modification system, independent of PA1939. Moreover, a 12 kb RPG42 prophage and a novel 108 kb PAPI-1 like integrative conjugative element (ICE) encompassing a mercury resistance operon were identified. The ICEPae1161 was transferred to Pseudomonas putida cells, where it integrated in the genome and conferred the mercury resistance. Conclusions: The high-quality P. aeruginosa PAO1161 genome sequence provides a reference for further research including e.g. investigation of horizontal gene transfer or comparative genomics. The strain was found to carry ICEPae1161, a functional PAPI-1 family integrative conjugative element, containing loci conferring mercury resistance, in the past attributed to the FP2 plasmid of IncP-8 incompatibility group. This indicates that the only known member of IncP-8 is in fact an ICE.

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Section: Introductionmentioning

confidence: 99%

Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element

et al. 2020

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“…Among various bacterial species, resistance to aminoglycosides arises through a variety of intrinsic and acquired mechanisms [11]. P. aeruginosa has developed several mechanisms to resist antimicrobial agents that can be associated with high mortality [12,2].…”

Section: Introductionmentioning

confidence: 99%

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

AL-Sheikhly

Musleh

Al-Mathkhury

2020

eijs

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Pseudomonas aeruginosa produces an extracellular biofilm matrix that consists of nucleic acids, exopolysaccharides, lipid vesicles, and proteins. Alginate, Psl and Pel are three exopolysaccharides that constitute the main components in biofilm matrix, with many biological functions attributed to them, especially concerning the protection of the bacterial cell from antimicrobial agents and immune responses. A total of 25 gentamicin-resistant P. aeruginosa selected isolates were enrolled in this study. Biofilm development was observed in 96% of the isolates. In addition, the present results clarified the presence of pelA and pslA in all the studied isolates. The expression of these genes was very low. Even though all biofilms were affected by gentamicin, the results of fold change showed a wide variation. In conclusion, all P. aeruginosa isolates carried psl and pel regardless of the intensity of the biofilm. A strongly positive correlation with gentamicin minimum inhibitory concentration was noticed. ‫ل‬ ‫الجيني‬ ‫التعبير‬pelA ‫و‬ pslA ‫في‬ ‫الزنجارية‬ ‫ائف‬ ‫الزو‬ ‫الجنتامايسين‬ ‫اجهاد‬ ‫عند‬ ‫حسن‬ ‫الزساق‬ ‫عبد‬ ‫محمود‬ 1 , 2 ‫مصلح‬ ‫نجيب‬ ‫,ليث‬ 2 ‫المذخوري‬ ‫فهد‬ ‫جبار‬ ‫حارث‬ , 3 1 ‫ات،‬ ‫السختبر‬ ‫قدػ‬ ‫ال‬ ‫األمؽر‬ ‫ة‬ ‫دائر‬ ‫اق‬ ‫العر‬ ‫بغداد،‬ ‫الرحة،‬ ‫ة‬ ‫ار‬ ‫وز‬ ‫فشية،‬ 2 ‫اق‬ ‫العر‬ ‫االنبار،‬ ‫االنبار،‬ ‫جامعة‬ ‫العلؽم،‬ ‫كلية‬ ‫الحياة،‬ ‫علؽم‬ ‫قدػ‬ 3 ‫اق‬ ‫العر‬ ‫بغداد،‬ ‫بغداد،‬ ‫جامعة‬ ‫العلؽم،‬ ‫كلية‬ ‫الحياة،‬ ‫علؽم‬ ‫قدػ‬ ‫الخالصه‬ ‫و‬ ‫نؽوية‬ ‫احساض‬ ‫مؼ‬ ‫يتألف‬ ‫خلؽي‬ ‫خارج‬ ‫حياتي‬ ‫غذاء‬ ‫قالب‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫تشتج‬ ‫يد‬ ‫الدكر‬ ‫عديد‬ ‫الخارجي‬ ‫و‬ ‫االلجيشات‬ ‫تعد‬ ‫بروتيشات.‬ ‫و‬ ‫دىشية‬ ‫وحؽيرالت‬ psl ‫و‬ pel ‫في‬ ‫األىػ‬ ‫يد‬ ‫الدكر‬ ‫عديد‬ ‫مؼ‬ ‫ثالثة‬ ‫و‬ ‫الحياتي‬ ‫الغذاء‬ ‫تركيب‬ ‫يزطلع‬ ‫الذي‬ ‫السزادات‬ ‫مؼ‬ ‫ية‬ ‫البكتير‬ ‫الخلية‬ ‫حساية‬ ‫وخرؽصا‬ ‫عديدة‬ ‫حياتية‬ ‫بؽظائف‬ ‫مجسؽعو‬ ‫ما‬ ‫اسة‬ ‫الدر‬ ‫ليذه‬ ‫اختير‬ ‫السشاعي.‬ ‫الجياز‬ ‫و‬ ‫الحياتية‬ 22 ‫السقاومة‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫مؼ‬ ‫لة‬ ‫عز‬ ‫في‬ ‫الحياتي‬ ‫الغذاء‬ ‫تكؽن‬ ‫لؽحظ‬ ‫للجشتامايديؼ.‬ 69 ‫مؼ‬ ‫كل‬ ‫وجؽد‬ ‫الحالية‬ ‫الشتائج‬ ‫أوضحت‬ ‫كسا‬ ‫الت.‬ ‫العز‬ ‫مؼ‬ % pslA ‫و‬ pelA ‫جدا.‬ ‫مشخفزا‬ ‫الجيشيؼ‬ ‫ليذيؼ‬ ‫الجيشي‬ ‫التعبير‬ ‫كان‬ ‫ذلغ‬ ‫على‬ ‫عالوة‬ ‫اسة.‬ ‫الدر‬ ‫الت‬ ‫عز‬ ‫جسيع‬ ‫في‬ ‫قد‬ ‫الجشتامايديؼ‬ ‫مؼ‬ ‫الرغػ‬ ‫على‬ ‫كان‬ ‫الجيشي‬ ‫التعبير‬ ‫في‬ ‫ه‬ ‫تاثير‬ ‫ان‬ ‫اال‬ ‫الت،‬ ‫العز‬ ‫لكل‬ ‫الحياتي‬ ‫الغذاء‬ ‫في‬ ‫اثر‬ ‫كل‬ ‫تستلغ‬ ‫ية‬ ‫نجار‬ ‫الز‬ ‫ائف‬ ‫الزو‬ ‫الت‬ ‫عز‬ ‫جسيع‬ ‫ان‬ ‫اسة‬ ‫الدر‬ ‫ىذه‬ ‫مؼ‬ ‫ندتشتج‬ ‫ان‬ ‫السسكؼ‬ ‫مؼ‬ ‫اسعة.‬ ‫و‬ ‫بدرجة‬ ‫متغاير‬ 296 ‫مؼ‬ pslA ‫و‬ pelA ‫التر‬ ‫بيؼ‬ ‫ضعيف‬ ‫تباط‬ ‫ار‬ ‫وجؽد‬ ‫لؽحظ‬ ‫كسا‬ ‫الحياتي.‬ ‫الغذاء‬ ‫قؽة‬ ‫و‬ ‫شدة‬ ‫عؼ‬ ‫الشعر‬ ‫بغض‬ ‫كيز‬ ‫مؼ‬ ‫لكل‬ ‫الجيشي‬ ‫التعبير‬ ‫و‬ ‫للجشتامايديؼ‬ ‫األدنى‬ ‫السثبط‬ pslA ‫و‬ pelA .

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“…The system responsible for cell-to-cell communication in bacteria is mediated by QS, which relies on bacterial density to diffuse small signal molecules, N-acyl homoserine lactones (AHLs), that bind transcriptional activators to regulate gene expression. Studies report that QS controls expression of more than 300 genes involved in virulence factors synthesis, motility, phenotypic changes, biofilm formation, antibiotic resistance, and metabolic pathways that regulate stress response(5). P. aeruginosa has four QS systems: las, rhl, pqs, and iqs.…”

Section: Introductionmentioning

confidence: 99%

“…The rhl system produces N-butanoyl-L-homoserine lactone (C 4 -HSL) to modulate virulence gene expression (8). The pqs system regulates the production of 2-heptyl-3-hydroxy-4(1H)-quinolone or simple quinolone and has been reported to control biofilm formation and the production of virulence factors (5, 9). Finally, the iqs system produces 2-(2-hydroxyphenyl)-thiazole-4-carbaldehyde but its exact role requires additional research.…”

Section: Introductionmentioning

confidence: 99%

Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing inPseudomonas aeruginosa

Dai

TianQi²,

Wang

et al. 2019

Preprint

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23Pseudomonas aeruginosa is one of the leading causes of opportunistic and 24 hospital-acquired infections worldwide. The infection with P. aeruginosa is frequently 25 linked with clinical treatment difficulties given drug resistance and abuse of 26 antibiotics. Ibuprofen, a widely used non-steroidal anti-inflammatory drug, has been 27 previously reported to exert antimicrobial activity, although the specific mechanism of 28 its action requires additional investigation. Given the regulation effects on quorum 29 sensing (QS), we hypothesized that inhibition of P. aeruginosa with ibuprofen is 30 linked with the QS systems. First, we assessed the action of ibuprofen in P. 31 aeruginosa by measuring CFU. The antimicrobial activity of ibuprofen was evaluated 32 2 by crystal violent staining and acridine orange staining at various drug concentrations 33 (0, 50, 75, and 100 μg/mL). Moreover, the effect of ibuprofen on different QS 34 virulence factors, such as pyocyanin, elastase, protease, and rhamnolipids, was 35 assessed revealing a concentration-dependent decrease (P<0.05). The effect of 36 ibuprofen was confirmed by liquid chromatography/mass spectrometry analysis of 37 3-oxo-C 12 -HSL and C 4 -HSL production. In addition, qRT-PCR results identified 38 significant suppression of Las and Rhl gene expression after 18 hours of treatment 39 with ibuprofen (P<0.05), with the most significant suppression observed at the 40 concentration of 75 μg/mL. Functional complementation with exogenous 41 3-oxo-C 12 -HSL and C 4 -HSL suggested that C 4 -HSL can recover the production of 42 virulence factors and biofilm formation in P. aeruginosa. Molecular docking of 43 ibuprofen with QS-associated proteins revealed high binding affinity. In summary, the 44 results suggest that ibuprofen is a candidate drug for the treatment of clinical 45 infections with P. aeruginosa. 46 Keywords: P. aeruginosa, ibuprofen, antimicrobial activity, quorum sensing, N-acyl 47 homoserine lactones 48 49 Introduction 50 Pseudomonas aeruginosa, the most common Gram-negative non-fermentative 51 bacteria, is one of the leading causes of opportunistic and hospital-acquired infections 52 worldwide(1). The infection can cause serious complications in patients with burns, 53 cystic fibrosis, or in immunocompromised patients with respiratory infections, sepsis, 54 osteomyelitis, endocarditis, and urinary tract infections (UTIs) (2). The infection with 55 P. aeruginosa has been liked with difficulties in clinical treatment because of 56 significant resistance of the bacterium to antibiotics (3), which is further aggravated 57 by the worldwide abuse of antibiotics. Specifically, every year approximately 700,000 58 people die from antibiotic resistance worldwide, which can likely increase to 10 59 million by 2050 if no action is taken to reduce drug resistance or to develop new 60 antibiotics. In 2017, the World Health Organization (WHO) published a list of 61 drug-resistant bacteria threating the lives of people which needs require for which 62 3 new antibio...

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Updates on the pathogenicity status of Pseudomonas aeruginosa

Cited by 270 publications

References 95 publications

Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element

Genome sequence of Pseudomonas aeruginosa PAO1161, a PAO1 derivative with the ICEPae1161 integrative and conjugative element

Gene Expression of pelA and pslA in Pseudomonas Aeruginosa under Gentamicin Stress

Ibuprofen-mediated potential inhibition of biofilm development and quorum sensing inPseudomonas aeruginosa

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