UPLC-ESI/MS/MS Profiling and Anti-Inflammatory Activity of Gleditsia caspica

Abstract

The Caspian Locust is the common name of Gleditsia caspica, a tree cultivated in the southern and western Caspian Sea in Russia and Iran. Herein, a qualitative characterization of the chemical constituents of Gleditsia caspica leaves extract using ultra-performance liquid chromatography coupled to electrospray ionization tandem mass fragmentation (UPLC-ESI/MS/MS) analysis was performed revealing the presence of nine compounds: six flavonoids, one triterpenoid, one phytosterol, and one long chain ester. Six compounds were newly reported from Gleditsia caspica and two for the first time from genus Gleditsia. Moreover, the in-vivo anti-inflammatory activities of two different doses; 50 and 100 mg/kg of the aqueous-alcoholic extract of Gleditsia caspica leaves were evaluated in the carrageenaninduced paw edema model. The percentage of edema inhibition was calculated after 1, 2, 3, and 4 h of the administration showing a significant reduction in edema thickness in a dose and time-dependent manner compared to the control drug, indomethacin. These results put forward Gleditsia caspica extract as a potential natural anti-inflammatory agent with minimal side effects.

Potential anti‐obesity effect of saponin metabolites from adzuki beans: A computational approach

“…Natural products have been the source of bioactive molecules since antiquity with marked effectiveness against various ailments, such as inflammation, cancer, infection, bone diseases, and more (Ashraf et al., 2020 , 2022 ; Moussa et al., 2020 ; Fayez et al., 2022 ; Moussa, 2013 ). In Japan, V. angularis , known as adzuki beans, were considered a part of the traditional Kampo medicine, which prescribed its juice and decoction to reduce aging and cardiovascular diseases (CVDs) (Maruyama et al., 2008 ).…”