UPLC-Q-TOF/MS-based plasma metabolome to identify biomarkers and time of injury in traumatic brain injured rats

Abstract

Background To identify the potent metabolic biomarkers and time of injury of traumatic brain injured (TBI). Methods A total of 70 Sprague–Dawley rats were used to establish the TBI model in this study. The serum was collected at 3 h, 6 h, 12 h, 24 h, 3 days and 7 days after surgery. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was performed to analyze metabolic changes in the serum of the TBI rats from different groups. The differences between the metabolic profiles of the rats in seven groups were analyzed using partial least squares discriminant analysis. Results Metabolic profiling revealed significant differences between the sham-operated and other groups. A total of 49 potential TBI metabolite biomarkers were identified between the sham-operated group and the model groups at different time points. Among them, six metabolites (methionine sulfone, kynurenine, 3-hydroxyanthranilic acid, 3-Indolepropionic acid, citric acid and glycocholic acid) were identified as biomarkers of TBI to estimate the injury time. Conclusion Using metabolomic analysis, we identified new TBI serum biomarkers for accurate detection and determination of the timing of TBI injury.

HPV-associated cervicovaginal microbiome and host metabolome characteristics

“…Paired microbiome and metabolomics samples from the remaining 42 participants (17 in the normal group and 25 in the HPV group) were subjected to microbiome and metabolome analyses. All HPV(+) subjects had infected one of 14 high-risk HPV genotypes: 16,18,31,33,35,39,45,51,52,56,58,59, 66 and 68 (Supplementary Table 1). Seven of them had mixed infection of both low-risk and high-risk HPV (Supplementary Table 1).…”

“…The correlation between placental ethylparaben and cord blood gammaglutamate transferase (GGT) and glucose levels provides a starting point for further studies on the mechanisms of uterine metabolic processes associated with parabens [50]. Glycocholine was used as a metabolic biomarker for traumatic brain injury (TBI) via assessing the duration of TBI injury [51]. Glycocholic acid metabolism was closely associated with certain strains of Bacteroides [52].…”

The alteration of serum bile acid profile among traumatic brain injury patients: a small-scale prospective study

“…In 2021, researchers reported that GCA was able to function as a biomarker in TBI rat models to estimate the injury time. ( 25 )…”

“…function as a biomarker in TBI rat models to estimate the injury time. (25) Apart from pre-clinical results, there also have been clinical studies that connecting the decreased bile acids with neurological diseases. (17,26,27) In schizophrenia patients, TCDCA and other two bile acids were reported to be significantly decreased in plasma, and might be able to function as biomarkers.…”