UPLC-Q-TOF-MS Study of the Mechanism of THSWD for Breast Cancer Treatment

Abstract

Taohong Siwu decoction (THSWD) is a classic traditional Chinese medicine (TCM) prescription that is widely used in the clinical treatment of gynecological and cerebrovascular diseases. Here we used a method that coupled ultra-performance liquid chromatography to quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) in which both positive and negative ion modes were established to investigate the major constituents in THSWD. A Waters ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 mm) was used to separate the aqueous extract of THSWD. The mobile phase consisted of 0.1% aqueous formic acid (A) and acetonitrile (B). Ninety-five components were identified in two different ion modes, including aromatic acids, flavones, polysaccharides, volatile oils monoterpene glycosides, aromatic cyanogenic glycosides, and others. Pathological changes in tumors and serum expression of interleukin-4 in a mouse model of breast cancer were detected after THSWD treatment. The results showed that THSWD had obvious therapeutic effects. This study establishes a material basis for the use of THSWD in the treatment of breast cancer.

Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

“…Therefore, it is important to evaluate the pharmacokinetics of THSWD in vivo. We previously studied the efficacy of THSWD, and showed that THSWD promoted blood circulation, protected against cerebral ischemia, and relieved pain [11,12], and we confirmed that THSWD has a definite therapeutic effect on breast cancer, and studied its mechanism of action [13]. However, few studies have identified active substances absorbed from THSWD.…”

Taohong Siwu Decoction Ameliorates Ischemic Stroke Injury Via Suppressing Pyroptosis

“…Their respective contents of hydroxysafflor yellow A, amygdalin, paeoniflorin, ferulic acid, verbascoside, and ligustilide in THSWD were identified as 0.198, 0.45, 0.602, 0.031, 0.014, and 0.256 mg ml −1 (Chen et al, 2020). Also, our previous report has investigated the major constituents of THSWD by UPLC-Q-TOF-MS. A total of 95 components have been identified, including aromatic acids, flavonoids, polysaccharides, volatile oils, monoterpene glycosides, aromatic cyanoglycosides (Duan et al, 2019). Many published articles have confirmed that they are the basis of THSWD inhibitors of pyroptosis (Liu et al, 2017;Ye et al, 2020;Yin et al, 2020).…”

Polypharmacology-based Approach for Screening of Traditional Chinese Medicinal extract and Multi-dimensional Targeting Verification

“…According to the degree of node correlation, the order was as follows: Isatdis Radix (4th, yang tonic) [52], Forsythia Fructus (11th, Antipyretic and antidote) [53], Ginkgo Folium (5th, Antitussive and antiasthmatic drugs) [54], Mori Cortex (3rd, Antitussive and antiasthmatic drugs) [55], Licorice (1st, reinforcing drugs) [56] and Radix Salviae (2nd, Drugs with the e cacy of modifying rheological properties of blood) [57]. Aqueous extract of Chinese medicinal compound is the most common method to study compound components [58,59]. Hence, we sought points that were uniformly scattered on the domain [60,61], furthermore, we did a multi-nonlinear regression analysis to search for the optimal combination in preliminary experiments.…”