UPLC–QToF-MS Characterization of Blackberry Extracts of Cultivars ‘Tupy’, ‘Guarani’, and ‘Xavante’: Development of Extract-Loaded Niosomes

D’Angelo, Rebeca W. O.; Gonçalves, Melissa Marques; Fachi, Mariana Millan; Vilhena, Raquel de Oliveira; Pontarolo, Roberto; Maluf, Daniela Florencio

doi:10.1007/s43450-020-00076-8

Cited by 11 publications

(3 citation statements)

References 29 publications

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“…Blackberry fruits have been consumed for centuries thanks to their pleasant taste [ 1 ]. Furthermore, in recent years, berries have been proposed as a “superfood”, as they are an essential source of bioactive molecules such as vitamins A and C, carotenoids, sterols, terpenoids and phenolic compounds, with high bioactivity potential, and very low calorific content [ 2 , 3 , 4 ]. In the last few years, the identification of the bioactive molecules from blackberry fruits has been explored by various authors.…”

Section: Introductionmentioning

confidence: 99%

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Gil-Martínez

Mut-Salud

Ruiz-García

et al. 2023

Foods

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A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antioxidant capacity was assessed through Folin–Ciocalteu (31.05 ± 4.9 mg GAE/g d.w.), FRAP (637.8 ± 3.2 μmol Fe2+/g d.w.), DPPH (IC50 97.1 ± 2.4 μg d.w./mL) and TEAC (576.6 ± 8.3 μmol TE/g d.w.) assays. Furthermore, the extract exerted remarkable effects on in vitro cellular antioxidant activity in HUVEC cells at a concentration of 5 mg/mL. Antimicrobial activity of the extract was also tested. Most sensible microorganisms were Gram-positive bacteria, such as E. faecalis, B. cereus and Gram-negative E. coli (MBC of 12.5 mg/mL). IC50 values against colon tumoral cells HT-29 (4.9 ± 0.2 mg/mL), T-84 (5.9 ± 0.3 mg/mL) and SW-837 (5.9 ± 0.2 mg/mL) were also obtained. Furthermore, blackberry extract demonstrated anti-inflammatory activity inhibiting the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84) in a concentration-dependent manner. These results support that blackberry fruits are an interesting source of bioactive compounds that may be useful in the prevention and treatment of different diseases, mainly related to oxidative stress.

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Section: Introductionmentioning

confidence: 99%

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Gil-Martínez

Mut-Salud

Ruiz-García

et al. 2023

Foods

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“…Peak 25 at a retention time of 9.95 min, m / z 593.1508 (C 27 H 29 O 15 ) presented a fragment m / z 285.0404, which corresponds with luteolin-3-galactoside-7-rhamnoside [ 5 ]. At 10.14 min, the peak 27 at m / z 505.0982 (C 23 H 21 O 13 ) and fragment ions at m / z 151.0026, 301.0327, 447.094 was proposed to be quercetin hexose acetate, this compound has been detected previously in blackberry [ 25 ]. It must be emphasized that this compound has been identified for the first time in Cherimoya leaves.…”

Section: Resultsmentioning

confidence: 99%

Development of an Effective Sonotrode Based Extraction Technique for the Recovery of Phenolic Compounds with Antioxidant Activities in Cherimoya Leaves

Martín-García

Aznar-Ramos

Verardo

et al. 2022

Plants

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The leaves of Annona cherimola Mill (cherimoya) are a potential source of phenolic compounds that have been shown to have beneficial properties. Therefore, this study focuses on establishing an ultrasonic-assisted extraction of phenolic compounds in cherimoya leaves using a sonotrode. For that purpose, a Box-Behnken design based on a response surface methodology (RSM) was used to optimize factors, such as amplitude, extraction time and solvent composition to obtain the maximum content of phenolic compounds by HPLC-MS and the maximum in-vitro antioxidant activity by DPPH, ABTS and FRAP assays in ‘Fino de Jete’ cherimoya leaves. The optimal conditions were 70% amplitude, 10 min and 40:60 ethanol/water (EtOH/H2O) (v/v). The results obtained under these optimum conditions by using a sonotrode were compared with those from an ultrasonic bath; briefly, recovery of phenolic compounds by sonotrode was 2.3 times higher than a bath. Therefore, these optimal conditions were applied to different varieties ‘Campas’, ‘Fino de Jete’ and ‘Negrito Joven’ harvested in the Tropical Coast of Granada (Spain). A total of 39 phenolic compounds were determined in these cherimoya leaf extracts, 24 phenolic compounds by HPLC-MS and 15 proanthocianidins by HPLC-FLD. 5-p-coumaroylquinic acid, lathyroside-7-O-α-l-rhamnopyranoside and quercetin hexose acetate were first identified in cherimoya leaves. The most concentrated phenolic compounds were the flavonoids, such as rutin and quercetin hexoside and proanthocyanidins including monomers. Almost no significant differences in the phenolic content in these cultivars were found (11–13 mg/g d.w. for phenolic compounds and 11–20 mg/g d.w. for proanthocyanidins). In addition, sonotrode ultrasonic-assisted extraction has been shown to be an efficient extraction technique in the phenolic recovery from cherimoya leaves that could be implemented on an industrial scale.

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“…Diluted samples of the prepared niosomal suspension of Hyrtios extract (1:150 v/v ) were visualized using (JEM-1400, Jeol, Tokyo, Japan) adjusted at 80-kV. Niosomes were dried at 40 °C on copper grids left for 15 minutes before imaging [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Network Pharmacological Analysis of the Red Sea Sponge Hyrtios erectus Extract to Reveal Anticancer Efficacy of Corresponding Loaded Niosomes

Abou-Taleb¹,

Sayed

Refaat

et al. 2022

Marine Drugs

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In this study, the LC-HRMS-assisted chemical profiling of Hyrtios erectus sponge led to the annotation of eleven major compounds (1–11). H. erectus-derived crude extract (HE) was tested in vitro for its antiproliferative activity against three human cancer cell lines, Hep-G2 (human liver cancer cell line), MCF-7 (breast cancer cell line), and Caco-2 (colon cancer cell line), before and after encapsulation within niosomes. Hyrtios erectus extract showed moderate in vitro antiproliferative activities towards the studied cell lines with IC50 values 18.5 ± 0.08, 15.2 ± 0.11, and 13.4 ± 0.12, respectively. The formulated extract-containing niosomes (size 142.3 ± 10.3 nm, PDI 0.279, and zeta potential 22.8 ± 1.6) increased the in vitro antiproliferative activity of the entrapped extract significantly (IC50 8.5 ± 0.04, 4.1 ± 0.07, and 3.4 ± 0.05, respectively). A subsequent computational chemical study was performed to build a sponge–metabolite–targets–cancer diseases network, by focusing on targets that possess anticancer activity toward the three cancer types: breast, colon, and liver. Pubchem, BindingDB, and DisGenet databases were used to build the network. Shinygo and KEGG databases in addition to FunRich software were used for gene ontology and functional analysis. The computational analysis linked the metabolites to 200 genes among which 147 genes related to cancer and only 64 genes are intersected in the three cancer types. The study proved that the co-occurrence of compounds 1, 2, 3, 7, 8, and 10 are the most probable compounds possessing cytotoxic activity due to large number of connections to the intersected cytotoxic genes with edges range from 9-14. The targets possess the anticancer effect through Pathways in cancer, Endocrine resistance and Proteoglycans in cancer as mentioned by KEGG and ShinyGo 7.1 databases. This study introduces niosomes as a promising strategy to promote the cytotoxic potential of H. erectus extract.

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UPLC–QToF-MS Characterization of Blackberry Extracts of Cultivars ‘Tupy’, ‘Guarani’, and ‘Xavante’: Development of Extract-Loaded Niosomes

Cited by 11 publications

References 29 publications

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Development of an Effective Sonotrode Based Extraction Technique for the Recovery of Phenolic Compounds with Antioxidant Activities in Cherimoya Leaves

Network Pharmacological Analysis of the Red Sea Sponge Hyrtios erectus Extract to Reveal Anticancer Efficacy of Corresponding Loaded Niosomes

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