UPLC–TOF-MS for plant metabolomics: A sequential approach for wound marker analysis in Arabidopsis thaliana

Grata, Elia; Bernard, Julien; Guillarme, Davy; Glauser, Gaëtan; Carrupt, Pierre‐Alain; Farmer, Edward E.; Wolfender, Jean‐Luc; Rudaz, Serge

doi:10.1016/j.jchromb.2008.04.021

Cited by 97 publications

(50 citation statements)

References 49 publications

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“…Therefore, highthroughput separations can be obtained with short column lengths for metabolite fingerprinting, whereas highly efficient separations are achieved with relatively long columns for detailed metabolite profiling [32]. However, the use of such small particles requires dedicated systems that are able to work at pressures up to 1000 bars.…”

Section: Hplc Separation Of Crude Extractsmentioning

confidence: 99%

Modern Approaches in the Search for New Active Compounds from Crude Extracts of Natural Sources

Queiroz

Hostettmann

Wolfender

2012

Plant Bioactives and Drug Discovery

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Section: Hplc Separation Of Crude Extractsmentioning

confidence: 99%

Modern Approaches in the Search for New Active Compounds from Crude Extracts of Natural Sources

Queiroz

Hostettmann

Wolfender

2012

Plant Bioactives and Drug Discovery

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“…An untargeted methodology involved a two-step analytical approach, combining a fast metabolite fingerprinting phase and a high resolution metabolite profiling phase. [22,23] Chemometric and data mining tools were applied for visualization and differential analysis to survey metabolic changes related to defense signaling in the most comprehensive manner. [24,25] In an additional study, machine learning algorithms, including classification and feature selection methods (Neural Networks, Support Ve ctor Machines, Decision Trees, etc.…”

Section: Chemometrics and Data Mining: Advanced Analysis And Integratiomentioning

confidence: 99%

New Insights in Pharmaceutical Analysis

Guillarme

Schappler²,

Bernard³

et al. 2012

Chimia

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The research unit of pharmaceutical analytical chemistry (PAC) has been active in the field of separation sciences for many years. Liquid chromatography (LC) and its latest improvements such as ultra-high performance chromatography (UHPLC) and supercritical fluid chromatography (SFC) are deeply and thoroughly studied, from a fundamental viewpoint to its various application capabilities. Electro-driven separations such as capillary electrophoresis (CE) are also a major field of interest, especially for macromolecules, and low cost. All these techniques are investigated with various detection modes including mass spectrometry (MS) for various applications where high sensitivity and selectivity is needed. Extracting the relevant information from the overwhelming amount of data generated by modern analytical platforms has become an important issue for knowledge discovery in various research fields. The appropriate treatment of such data is therefore of crucial importance to provide valuable information. Numerous works in our research group have demonstrated the usefulness of statistical and mathematical methodologies to improve quality of the results. Therefore, well-established chemometric approaches (e.g. design of experiments, multivariate data analysis, etc.) are implemented to optimize the analytical process from method development to data analysis.

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“…2C) and subsequent microfractionation of the biomarkers were highlighted and characterised by microflow NMR as described above. [43] The same analytical strategy is currently being applied to study the effect of various phytopharmaceuticals with proven clinical efficacy for which classical reductionist bioactivity-guided fractionation approaches did not lead to the identification of the active ingredients. For such investigations, metabolomic data acquired on body fluids will be analysed from a holistic viewpoint after ingestion of the preparations and the bioactivity of metabolised phytonutrients will be measured.…”

Section: Reductionist Versus Holistic Approaches To Study the Health mentioning

confidence: 99%

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Wolfender

Eugster

Bohni

et al. 2011

Chimia

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Advances in analytical methods and bioassay development have helped to push forward the research in natural products. In plant extracts and nutraceuticals, bioactive compounds are part of a complex mixture. The development of high-resolution methods related to HPLC for both chemical and biological profiling has significantly increased the efficiency of classical bioactivity-guided fractionation procedures. Furthermore, the level of sensitivity obtained by these methods give the possibility to work with few micrograms of compound. This represents a key advantage for rapid localisation of the biological activity and subsequent identification of the compounds of interest. The same methods are also used to study the extracts from a metabolomic view point. The possibility to study them as a whole can highlight synergetic effects, which are likely to occur in plant extracts and nutraceuticals. In this paper, the main trends are summarised and the developments made in our laboratory on profiling crude extracts with UHPLC-TOF-MS, natural product identification at the microgram level using microflow NMR and integration of these methods with biological evaluation are highlighted.

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UPLC–TOF-MS for plant metabolomics: A sequential approach for wound marker analysis in Arabidopsis thaliana

Cited by 97 publications

References 49 publications

Modern Approaches in the Search for New Active Compounds from Crude Extracts of Natural Sources

Modern Approaches in the Search for New Active Compounds from Crude Extracts of Natural Sources

New Insights in Pharmaceutical Analysis

Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

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