Upregulated circ_0005576 facilitates cervical cancer progression via the miR-153/KIF20A axis

Ma, Hanyu; Tian, Tian; Liu, Xubin; Xia, Meng; Chen, Cancan; Mai, Lixiang; Xie, Shuhua; Yu, Li

doi:10.1016/j.biopha.2019.109311

Cited by 52 publications

(50 citation statements)

References 39 publications

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“…It was revealed that circ_0005576 could facilitate the progression of cervical cancer. 11 Unfortunately, more data on circ_0005576 affecting human disease development are not yet available. Our data demonstrate for the first time that circ_0005576 is an oncogene in CRC.…”

Section: Discussionmentioning

confidence: 99%

“…10 Circ_0005576 is a recently discovered circRNA, which has been found to facilitate cervical cancer progression by acting on the miR-153/KIF20A axis. 11 Circ_0005576 has therefore been proposed as an effective target for the therapeutic intervention in cervical cancer. However, no study has proven the role of circ_0005576 in the development of CRC as well as other human tumors.…”

Section: Introductionmentioning

confidence: 99%

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<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

Chen¹,

Li²,

Hu³

2020

OTT

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Purpose: To investigate the function of circ_0005576 in colorectal cancer (CRC) progression. Patients and Methods: Circ_0005576 expression in CRC patients was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization (ISH). CRC cells were transfected using Lipofectamine 2000 reagent. CRC cell proliferation was researched by Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2-deoxy-uridine (EdU) incorporation experiment. Cell cycle and apoptosis were determined by flow cytometry analysis. Luciferase reporter assay was used to explore the relationship between circ_0005576 and miR-874 or between miR-874 and CDK8. qRT-PCR and Western blot were used to detect circ_0005576, miR-874, and CDK8 expression. In vivo experiments were performed using nude mice. CDK8 and Ki67 expression in xenograft tumors was investigated by immunohistochemistry. Tunel assay was conducted to analyze the apoptosis of xenograft tumors. Results: Circ_0005576 expression was up-regulated in CRC, which was associated with tumor progression (P < 0.05 or P < 0.01). Circ_0005576 knockdown in CRC cells reduced proliferation, induced apoptosis, increased cells in the G1 phase, and decreased cells in the S phase (P < 0.01 or P < 0.001). Circ_0005576 promoted CDK8 expression via sponging miR-874. miR-874 knockdown and CDK8 overexpression significantly reversed the inhibitory effect of circ_0005576 knockdown on CRC cells malignant phenotype (P < 0.05 or P < 0.01). Circ_0005576 knockdown inhibited tumor growth in vivo (P < 0.01). Circ_0005576 knockdown reduced CDK8, Ki67 expression, and enhanced apoptosis in xenograft tumors. Conclusion: Circ_0005576 promoted malignant progression through the miR-874/CDK8 axis in CRC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

Chen¹,

Li²,

Hu³

2020

OTT

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“…In addition, has-circ-u0000745 was upregulated in cervical cancer tissues and cell lines, which was found to be correlated with various clinicopathological features, such as poorly differentiated tumors and positive vascular/lymphatic invasion (45). Another circRNA, circ_0005576, has been associated with a higher pathological stage and lymph node metastasis (50). However, alternative studies have indicated that reduced circRNA expression also promotes metastasis; Jiao et al (51) revealed that low expression levels of circRNA_101308 were associated with deep myometrial invasion and lymph node metastasis.…”

Section: Iii) Circrnas As Biomarkers Of Cervical Cancer Metastasismentioning

confidence: 98%

“…Previous research on circRNAs has improved our understanding of cervical cancer pathogenesis. Ma et al (50) recently discovered a novel circ_0005576-miR-153-3p-kinesin family member 20A (KIF20A) axis that regulates cervical cancer growth and invasion. They identified a potentially new form of circ_0005576 that is primarily located in the cytoplasm, and is significantly upregulated in cervical cancer.…”

Section: Iii) Circrnas As Biomarkers Of Cervical Cancer Metastasismentioning

confidence: 99%

Circular RNAs: Novel biomarkers for cervical, ovarian and endometrial cancer (Review)

Shi

et al. 2020

Oncol Rep

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Cervical, ovarian and endometrial cancer are the three most common types of malignant tumor and the leading causes of cancer-associated death in women. Tumor debulking surgery followed by platinum and paclitaxel chemotherapy is the current treatment regime of choice. However, as a result of late diagnosis and chemoresistance, the survival rates of patients with advanced gynecological cancers remains unsatisfactory. Circular RNAs (circRNAs) are stable noncoding RNAs that are present in a wide variety of tissue and cell types. With the enhancement of RNA sequencing methods, increasing numbers of circRNAs have been identified, and their functions are gradually being revealed. In recent years, circRNAs have received increasing attention for their regulatory roles in cervical, ovarian and endometrial cancer. The aim of the present review was to summarize the possible mechanisms of recently identified circRNAs; we hypothesize that a novel diagnostic and therapeutic biomarker may be identified to prolong the survival time of patients with gynecological malignancies.

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“…Then, 13 overlapped miRNAs of the two databases were found to potentially interact with hsa_circ_0001829. Among the 13 candidate miRNAs, we mainly focused on ve miRNAs (miR-153-3p, miR-144-5p, miR-194-5p, miR-155-5p and miR-564-5p), which have been reported to be closely related to cancer development (24)(25)(26)(27)(28). For further investigation, dual luciferase reporter assays were performed and demonstrated that relative luciferase activity was reduced by more than 30% when cells were cotransfected with hsa_circ_0001829 wild-type vector and miR-155-5p mimics, whereas co-transfection with the other four miRNAs mimics induced non-signi cant differences, suggesting that hsa_circ_0001829 interacts directly with miR-155-5p (Fig.…”

Section: Hsa_circ_0001829 Acts As a Molecular Sponge For Mir-155-5pmentioning

confidence: 99%

Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

Niu

Dong

Liang

et al. 2020

Preprint

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Background: Accumulating evidences have shown that circular RNAs (circRNAs) play important roles in regulating the pathogenesis of cancer. However, the role of circRNAs in gastric cancer (GC) remains largely unclear. Methods: In this study, we identified a novel upregulated circRNA, hsa_circ_0001829, in chemically induced malignant transformed human gastric epithelial cells using RNA-seq. Subsequent qRT-PCR was performed to detect the expression level of hsa_circ_0001829 in GC cell lines and tissues. Functional roles of hsa_circ_0001829 in GC were then explored by loss- and gain-of- function assays. Bioinformatic prediction and luciferase assay were used to investigate potential mechanisms of hsa_circ_0001829. Finally, the mice xenograft models were constructed to assess the function of hsa_circ_0001829 in vivo.Results: We found that hsa_circ_0001829 was significantly upregulated in GC tissues and cell lines. Loss- and gain-of- function assays showed that hsa_circ_0001829 promotes GC cells proliferation, migration and invasion, and the affected cell cycle progression and apoptosis rates may account for the effect of hsa_circ_0001829 on GC proliferation. In addition, bioinformatic prediction and luciferase assay showed that hsa_circ_0001829 acts as a molecular sponge for miR-155-5p and that SMAD2 was a target gene of miR-155-5p; moreover, hsa_circ_0001829 sponges miR-155-5p to regulate SMAD2 expression and hsa_circ_0001829 promotes GC progression through the miR-155-5p–SMAD2 pathway. Finally, suppression of hsa_circ_0001829 expression inhibited subcutaneous tumor growth in vivo. Conclusion: Taken together, our findings firstly demonstrated a novel oncogenic role of hsa_circ_0001829 in GC progression, which is through miR-155-5p–SMAD2 axis, and our study may offer novel biomarkers and therapeutic targets for GC.

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Upregulated circ_0005576 facilitates cervical cancer progression via the miR-153/KIF20A axis

Cited by 52 publications

References 39 publications

<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

Circular RNAs: Novel biomarkers for cervical, ovarian and endometrial cancer (Review)

Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

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Upregulated circ_0005576 facilitates cervical cancer progression via the miR-153/KIF20A axis

Cited by 52 publications

References 39 publications

<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

<p>Circ_0005576 Promotes Malignant Progression Through miR-874/CDK8 Axis in Colorectal Cancer</p>

Circular RNAs: Novel biomarkers for cervical, ovarian and endometrial cancer (Review)

Circular RNA hsa_circ_0001829 Promotes&nbsp;Gastric Cancer Progression through miR-155-5p/SMAD2 Axis

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Circular RNA hsa_circ_0001829 Promotes Gastric Cancer Progression through miR-155-5p/SMAD2 Axis