Upregulation of CD86 and IL-12 by rhododendrol in THP-1 cells cocultured with melanocytes through ROS and ATP

Katahira, Yasuhiro; Sakamoto, Eri; Watanabe, Aruma; Furusaka, Yuma; Inoué, Shinya; Hasegawa, Hideaki; Mizoguchi, Itaru; Yo, Kazuyuki; Yamaji, Fumiya; Toyoda, Akemi; Yoshimoto, Takayuki

doi:10.1016/j.jdermsci.2022.12.012

Cited by 4 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salmonella LPS is a robust stimulator of the innate immune system. Stimulation of monocytes with purified LPS increases expression of costimulatory molecules CD80 and CD86 and promotes maturation into macrophages (Katahira et al., 2022; Mytych et al., 2017). There is a distinction in the activation properties between the markers CD80 and CD86.…”

Section: Introductionmentioning

confidence: 99%

Controlled production of lipopolysaccharides increases immune activation in Salmonella treatments of cancer

Howell,

Manole,

Reitter

et al. 2024

Microbial Biotechnology

View full text Add to dashboard Cite

Immunotherapies have revolutionized cancer treatment. These treatments rely on immune cell activation in tumours, which limits the number of patients that respond. Inflammatory molecules, like lipopolysaccharides (LPS), can activate innate immune cells, which convert tumour microenvironments from cold to hot, and increase therapeutic efficacy. However, systemic delivery of lipopolysaccharides (LPS) can induce cytokine storm. In this work, we developed immune‐controlling Salmonella (ICS) that only produce LPS in tumours after colonization and systemic clearance. We tuned the expression of msbB, which controls production of immunogenic LPS, by optimizing its ribosomal binding sites and protein degradation tags. This genetic system induced a controllable inflammatory response and increased dendritic cell cross‐presentation in vitro. The strong off state did not induce TNFα production and prevented adverse events when injected into mice. The accumulation of ICS in tumours after intravenous injection focused immune responses specifically to tumours. Tumour‐specific expression of msbB increased infiltration of immune cells, activated monocytes and neutrophils, increased tumour levels of IL‐6, and activated CD8 T cells in draining lymph nodes. These immune responses reduced tumour growth and increased mouse survival. By increasing the efficacy of bacterial anti‐cancer therapy, localized production of LPS could provide increased options to patients with immune‐resistant cancers.

show abstract

Section: Introductionmentioning

confidence: 99%

Controlled production of lipopolysaccharides increases immune activation in Salmonella treatments of cancer

Howell,

Manole,

Reitter

et al. 2024

Microbial Biotechnology

View full text Add to dashboard Cite

show abstract

The molecular mechanisms underlying optical isomer arbutin permeating the skin: The molecular interaction between arbutin and skin components

Li,

Wang,

Zhou

et al. 2024

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Chemical- and Drug-Induced Allergic, Inflammatory, and Autoimmune Diseases Via Haptenation

Sakamoto

Katahira

Mizoguchi

et al. 2023

Biology

View full text Add to dashboard Cite

Haptens are small molecules that only elicit an immune response when bound to proteins. Haptens initially bind to self-proteins and activate innate immune responses by complex mechanisms via inflammatory cytokines and damage-associated molecular patterns and the subsequent upregulation of costimulatory signals such as cluster of differentiation 86 (CD86) on dendritic cells. Subsequent interactions between CD86 and CD28 on T cells are critically important for properly activating naive T cells and inducing interleukin 2 production, leading to the establishment of adaptive immunity via effector and memory T cells. Accumulating evidence revealed the involvement of haptens in the development of various autoimmune-like diseases such as allergic, inflammatory, and autoimmune diseases including allergic contact dermatitis, atopy, asthma, food allergy, inflammatory bowel diseases, hemolytic anemia, liver injury, leukoderma, and even antitumor immunity. Therefore, the development of in vitro testing alternatives to evaluate in advance whether a substance might lead to the development of these diseases is highly desirable. This review summarizes and discusses recent advances in chemical- and drug-induced allergic, inflammatory, and autoimmune diseases via haptenation and the possible molecular underlying mechanisms, as well as in vitro testing alternatives to evaluate in advance whether a substance might cause the development of these diseases.

show abstract

Upregulation of CD86 and IL-12 by rhododendrol in THP-1 cells cocultured with melanocytes through ROS and ATP

Cited by 4 publications

References 46 publications

Controlled production of lipopolysaccharides increases immune activation in Salmonella treatments of cancer

Controlled production of lipopolysaccharides increases immune activation in Salmonella treatments of cancer

The molecular mechanisms underlying optical isomer arbutin permeating the skin: The molecular interaction between arbutin and skin components

Chemical- and Drug-Induced Allergic, Inflammatory, and Autoimmune Diseases Via Haptenation

Contact Info

Product

Resources

About