Upregulation of Holliday junction recognition protein predicts poor prognosis and biochemical recurrence in patients with prostate cancer

Abstract

Abnormal expression of Holliday junction recognition protein (HJURP) in several types of tumor cells plays a vital role in the formation and progression of tumors. Few studies have investigated the role of HJURP in prostate cancer (PCa). The aim of this study was to analyze the expression levels of HJURP in PCa and to establish the association with clinicopathological data. Reverse transcription quantitative polymerase chain reaction and immunohistochemical analysis were used to detect the expression levels of HJURP in benign and PCa prostate tissues. The Taylor dataset was statistically analyzed to determine if HJURP expression levels were associated with PCa clinicopathological data. HJURP was overexpressed in PCa tissues compared with benign prostate tissues. Statistical analysis of the Taylor dataset indicated that upregulation of HJURP was significantly associated with positive prostate-specific antigen (PSA) levels (P=0.004), high Gleason score (P=0.005), advanced pathological stage (P=0.007), metastasis (P<0.001) and PSA failure (P<0.001). Higher HJURP mRNA expression levels were significantly associated with shorter biochemical recurrence (BCR)-free survival (P<0.001). To the best of our knowledge, this study is the first report of HJURP upregulation in PCa tissues. Upregulation of HJURP may predict BCR-free survival and HJURP may be an oncogene that impacts the prognosis of patients with PCa.

HJURP promotes proliferation in prostate cancer cells through increasing CDKN1A degradation via the GSK3β/JNK signaling pathway

“…Interestingly, the subcellular location of HJURP was initially reported to be nucleus 4 , however, we found that it was mainly stained in the cytoplasm of PCa cells according to the IHC. This is consistent with prior studies in PCa 10 . In addition, in hepatocellular carcinoma 12 , 26 , HJURP is also stained in the cytoplasm.…”

“…Similarly, HJURP is an unfavorable factor in PCa 10 , but the specific functions and mechanisms underlying its involvement remain unclear. In this study, we demonstrated that HJURP was upregulated in PCa and its expression was positively correlated with unfavorable outcomes.…”

Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma

“…The expression of HJURP was downregulated at the mRNA and protein expression levels in RCC cell lines and tissues. This result is in contrast with studies on other types of cancer (4,(14)(15)(16), suggesting that the specific role of HJURP varies and is tissue-dependent, and may be associated with organ specificity, or highlights the possibility that HJURP may exert its effects on different target genes in different tissues (36)(37)(38). In the present study, overexpression of HJURP in RCC cells reduced colony formation and cell growth.…”

To Identify and Verify the Role and Crucial Genes of Methionine Restriction in Inhibiting the Progression of Colon Cancer Cells

“…HJURP is a histone chaperone involved in the recruitment of de novo histone H3 variants CenH3 (CENP-A) and CENP-C in nucleosomes, which can regulate the ampli cation of centromeric chromatin and chromosomal stability [36] . HJURP has been con rmed as a key prognostic-related gene in breast cancer, hepatocellular carcinoma, advanced serous ovarian cancer, colorectal cancer and prostate cancer [37][38][39][40][41] . There are reports showing that HJURP promotes tumor cell proliferation.…”