2017
DOI: 10.18632/oncotarget.16561
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Upregulation of long non-coding RNA HOXA-AS2 promotes proliferation and induces epithelial-mesenchymal transition in gallbladder carcinoma

Abstract: Gallbladder carcinoma (GBC) is the most common malignancy of the bile duct and patients with GBC have extremely poor prognoses. Increasing evidence indicates that long non-coding RNAs (lncRNAs) regulate diverse cellular processes, including cell growth, differentiation, apoptosis, and cancer progression. However, the function of lncRNAs in the progression of GBC remains largely unknown. Here, we reported that HOXA cluster antisense RNA2 (HOXA-AS2) was upregulated in GBC. In vitro experiments revealed that HOXA… Show more

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Cited by 36 publications
(39 citation statements)
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“…In vitro and in vivo studies [23] showed that HOXA-AS2 overexpression promotes cell proliferation in gastric cancer. In addition, Zhang et al [24] demonstrated that HOXA-AS2 overexpression promotes tumor cell proliferation and induces epithelial-mesenchymal transition (EMT) in gallbladder carcinoma. It has been confirmed that lncRNAs regulate tumor progression through modulating gene expression at the epigenetic, transcriptional and posttranscriptional levels, in which interaction with miRNAs is important.…”
Section: Discussionmentioning
confidence: 99%
“…In vitro and in vivo studies [23] showed that HOXA-AS2 overexpression promotes cell proliferation in gastric cancer. In addition, Zhang et al [24] demonstrated that HOXA-AS2 overexpression promotes tumor cell proliferation and induces epithelial-mesenchymal transition (EMT) in gallbladder carcinoma. It has been confirmed that lncRNAs regulate tumor progression through modulating gene expression at the epigenetic, transcriptional and posttranscriptional levels, in which interaction with miRNAs is important.…”
Section: Discussionmentioning
confidence: 99%
“…The isolated proteins were then quantified and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and wet membrane transfer experiments were implemented in consonance with previous research. [ 14 ] Antibodies of cleaved caspase‐3 (#9661), cleaved caspase‐9 (#52873), TNF‐α (#3707), IL‐1β (#12242), IL‐6 (#12153; Cell Signaling Technology, Danvers, MA), Wnt3a (ab219412), Wnt5a (ab174963), β‐catenin (ab32572), p‐p65 (ab86299), t‐p65 (ab32536), p‐IκBα (ab133426), t‐IκBα (ab7217), and β‐actin (ab227387; Abcam, Cambridge, UK) were coincubated with the above membranes, which were sealed with bovine serum albumin for 1 hour. The corresponding secondary antibodies (#7074; Cell Signaling Technology and ab150117; Abcam) were utilized and reacted with membranes for an extra 1 hour at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…[ 11 ] HOXA cluster antisense RNA 2 (HOXA‐AS2) is a lncRNA with a length of 1048, which is situated between the HOXA3 and HOXA4 genes. [ 12 ] Much of the research on HOXA‐AS2 has focused on cancers, including gastric cancer, [ 13 ] gallbladder cancer [ 14 ] and colorectal cancer. [ 15 ] However, little research has been done on the latent role of HOXA‐AS2 in sepsis‐engendered AKI.…”
Section: Introductionmentioning
confidence: 99%
“…It is well known that inflammation is critically associated with osteogenesis. Moreover, recent studies showed the lncRNA HOXA‐AS2 plays a regulatory role in inflammation‐linked cancers . Therefore, we suggested HOXA‐AS2 is involved in the regulation of osteogenic differentiation.…”
Section: Resultsmentioning
confidence: 81%
“…Based on the prior studies that HOXA‐AS2 is implicated in inflammation‐linked cancers, we suggested that HOXA‐AS2 regulates the osteogenic differentiation via mediating NF‐κB activity. Western blot analysis showed HOXA‐AS2 could suppress the transcriptional activity of NF‐κB by inhibiting K310 acetylation of RelA, which identified HOXA‐AS2 as a crucial regulator of NF‐κB activation.…”
Section: Discussionmentioning
confidence: 99%