2019
DOI: 10.1186/s12872-019-1120-5
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Upregulation of miR-330-5p is associated with carotid plaque’s stability by targeting Talin-1 in symptomatic carotid stenosis patients

Abstract: Background The aim of this study was to investigate the relationship between Talin-1 and stability of carotid atherosclerosis plaque and also find out the role of miRNA, as an upstream regulator, in regulating the expression level of Talin-1. Methods Human carotid plaques were obtained from 20 symptomatic carotid stenosis patients who underwent carotid endarterectomy (CEA) in our hospital between October 2014 and August 2017. Western blot analysis and immunohistochemist… Show more

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Cited by 31 publications
(32 citation statements)
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“…Afterward, PCR was conducted by SYBR Premix Ex Taq Reagent (Takara) using U6 as the internal reference. The relative expression of miR‐126 was calculated by 2 −△△Ct method 13 . The primers used were as follows: miR‐126 forward primer: 5′‐ACACTCCAGCTGGGCATTATTACTTTTGGTAC‐3′; reverse primer: 5′‐ACACTCCAGCTGGGACTGCAGTGAAGGCACTT‐3′; U6 forward primer: 5′‐CTCGCTTCGGCAGCACATATACTA‐3′; reverse primer: 5′‐ACGAATTTGCGTGTCATCCTTGC‐3′.…”
Section: Methodsmentioning
confidence: 99%
“…Afterward, PCR was conducted by SYBR Premix Ex Taq Reagent (Takara) using U6 as the internal reference. The relative expression of miR‐126 was calculated by 2 −△△Ct method 13 . The primers used were as follows: miR‐126 forward primer: 5′‐ACACTCCAGCTGGGCATTATTACTTTTGGTAC‐3′; reverse primer: 5′‐ACACTCCAGCTGGGACTGCAGTGAAGGCACTT‐3′; U6 forward primer: 5′‐CTCGCTTCGGCAGCACATATACTA‐3′; reverse primer: 5′‐ACGAATTTGCGTGTCATCCTTGC‐3′.…”
Section: Methodsmentioning
confidence: 99%
“…miR-126-5p promotes endothelial cell proliferation and curbs the progression of AS through suppressing Dlk1 [25]. Up-regulated miR-330-5p is connected to the stability of carotid plaque via targeting Talin-1 [26].…”
Section: Discussionmentioning
confidence: 99%
“…These findings thus suggest that talin-1 would be downregulated in atherosclerotic plaques. Furthermore, talin-1 was reported to be downregulated in unstable carotid plaques compared with stable plaques [9]. The overexpression of microRNA-330-5p was also demonstrated in unstable plaques, which significantly downregulated talin-1 [9].…”
Section: Disease Markersmentioning
confidence: 97%
“…Furthermore, talin-1 was reported to be downregulated in unstable carotid plaques compared with stable plaques [9]. The overexpression of microRNA-330-5p was also demonstrated in unstable plaques, which significantly downregulated talin-1 [9]. In contrast, high TLN1 expression along with low microRNA-330-5p expression was reported in hepatocellular cancer tissues [19].…”
Section: Disease Markersmentioning
confidence: 99%
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