Upregulation of phospholipase D in astrocytes in response to transient forebrain ischemia

Lee, Mun Yong; Kim, Seong Yun; Min, Do Sik; Choi, Yun Sik; Shin, Soon Lim; Chun, Myung-Hoon; Lee, Sang Bok; Kim, Myung Suk; Jo, Yang-Hyeok

doi:10.1002/(sici)1098-1136(200005)30:3<311::aid-glia10>3.0.co;2-k

Cited by 50 publications

(20 citation statements)

References 33 publications

(30 reference statements)

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“…[13] PLDs have been implicated in a variety of cellular processes, including inflammation, secretion, and the respiratory burst in neutrophils. However, little is known about the involvement of PLD1 in models of PNS disease.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, antisera were raised against the C-terminal peptide of PLD1 corresponding to amino acid residues 1063-1074 of the sequence of PLD1 (TKEAIVPMEVWT), and characterized. [13] T cells and macrophages were identified using W3=25 and ED1 (Serotec, London, U.K.), respectively. Rabbit anti-S100 (1:800) (Dako, Copenhagen, Denmark) was used to identify Schwann cells.…”

Section: Immunohistochemistrymentioning

confidence: 95%

“…[8] Thus, the PA and DAG formed via PLD1 activation may be involved in a wide range of pathophysiological processes, including inflammation, secretion, mitogenesis, apoptosis, and the respiratory burst in neutrophils. [3,6,13] This implies that PLD1 has either beneficial or detrimental properties in cells, depending on the state of the stimulated cells.…”

Section: Introductionmentioning

confidence: 99%

“…[8] Several studies have shown that PLD1 is expressed in glial cells, such as presumed astrocytes in the rat central nervous system, [3] and that PLD1 is upregulated in astrocytes in response to transient forebrain ischemia [13] and in spinal cords in experimental autoimmune encephalomyelitis. [1] Little is known about the expression of PLD1 isozyme in peripheral nervous tissue.…”

Section: Introductionmentioning

confidence: 99%

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Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Shin

Min

Ahn

et al. 2002

Immunological Investigations

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Phospholipase D1 (PLD1) expression in the sciatic nerve was studied in induced experimental autoimmune neuritis (EAN) in Lewis rats. PLD1 immunoreactivity was seen in some Schwann cells in the sciatic nerves of normal rats. In parallel with the progression of EAN, PLD1-positive Schwann cells significantly increased in number and showed intense immunoreactivity. PLD1 was also detected in some ED1+ macrophages in EAN lesions. These results suggest that PLD1 in macrophages and Schwann cells plays an important role in the activation of these cells in the pathogenesis of EAN, an animal model of human peripheral demyelinating disease.

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Shin

Min

Ahn

et al. 2002

Immunological Investigations

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“…Analysis of mRNAs in the developing brain point to the astrocytes, for PLD2, and oligodendrocytes, for PLD1, as the main cell types enriched in PLDs [32]. In the adult rat, PLD1 was found to be upregulated in astrocytes in response to transient ischemia [33]. Considering that the mammalian brain is one of the organs with the highest specific activity of PLD, that neurons are not the major sites of PLD enzymes is rather unexpected.…”

Section: Expression Of Pld Isoformsmentioning

confidence: 99%

Signalling roles of mammalian phospholipase D1 and D2

Cockcroft

2001

CMLS, Cell. Mol. Life Sci.

190

134

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Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholine to generate the lipid second messenger, phosphatidate (PA) and choline. PLD activity in mammalian cells is low and is transiently stimulated upon activation by G-protein-coupled and receptor tyrosine kinase cell surface receptors. Two mammalian PLD enzymes (PLD1 and PLD2) have been cloned and their intracellular regulators identified as ARF and Rho proteins, protein kinase Calpha as well as the lipid, phosphatidylinositol bisphosphate (PIP2). I discuss the regulation of these enzymes by cell surface receptors, their cellular localisation and the potential function of PA as a second messenger. Evidence is presented for a role of PA in regulating the lipid kinase activity of PIP 5-kinase, an enzyme that synthesises PIP2. A signalling role of phospholipase D via PA and indirectly via PIP2 in regulating membrane traffic and actin dynamics is indicated by the available data.

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Upregulation of gp130 and STAT3 activation in the rat hippocampus following transient forebrain ischemia

Choi

Kim

Cha

et al. 2003

Glia

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To determine whether the pathophysiological processes after transient forebrain ischemia are mediated via a signal pathway involving gp130 (a signal transducer for the interleukin-6 family), we analyzed changes in the expression of gp130 and its downstream transcription factor, signal transducer and activator of transcription factor 3 (STAT3), in the rat hippocampus of a four-vessel occlusive ischemia model. Expression of gp130 mRNA was restricted to neurons of the pyramidal cell and granule cell layers in control animals. Four hours after ischemic injury, astrocytes expressed gp130 mRNA. Expression of gp130 increased preferentially in the CA1 and dentate hilar regions, and was maintained for at least 2 weeks. Increase in gp130 expression was accompanied by the activation of STAT3 following ischemic injury. Four hours after injury, STAT3 and phosphorylated STAT3 (pSTAT3) were observed in the nuclei of the dentate hilar region, and sequentially in the CA1 region at day 1. By day 3, STAT3 immunoreactivity markedly increased in these areas, where small cells with the morphology of astrocytes showed nuclear and cytoplasmic STAT3 and nuclear pSTAT3 immunoreactivities. These patterns were especially maintained in the CA1 area until 14 days of reperfusion. Double-labeling experiments revealed that the cells expressing STAT3 and pSTAT3 were glial fibrillary acidic protein-expressing reactive astrocytes. These results show a coordinated and long-lasting upregulation of gp130 mRNA and STAT3 activation in reactive astrocytes of the postischemic hippocampus, indicating that they may be involved in the astrocytic response to an ischemic insult.

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Upregulation of phospholipase D in astrocytes in response to transient forebrain ischemia

Cited by 50 publications

References 33 publications

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Increased Expression of Phospholipase D1 in the Sciatic Nerve of Rats With Experimental Autoimmune Neuritis

Signalling roles of mammalian phospholipase D1 and D2

Upregulation of gp130 and STAT3 activation in the rat hippocampus following transient forebrain ischemia

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