Upregulation of SERT and ADORA1 in broilers with acute right ventricular failure

Kamely, Mohammad; Torshizi, Mohammad Amir Karimi; Wideman, Robert F.; West, James

doi:10.1016/j.rvsc.2017.09.030

Cited by 5 publications

(6 citation statements)

References 37 publications

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“…Gene expression was performed as previously described ( 17 , 20 ). Briefly, abdominal lavage cells were collected with RPMI 1640 and centrifuged and kept within 1 mL Trizol reagent (Invitrogen Life Technologies) in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

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Impact of Reduced Dietary Crude Protein in the Starter Phase on Immune Development and Response of Broilers Throughout the Growth Period

Kamely

Wakaruk

et al. 2020

Front. Vet. Sci.

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Crude protein (CP) levels in commercial broiler (Gallus gallus) diets, optimized for maximum yield production vs. feed cost, have only begun to be assessed for impact on immune function. In order to study immune effects of dietary CP levels, different starter phase (day 1-14) diets were fed to 230 Ross 708 male broiler chicks randomly assigned at 1 day of age into two treatment groups. Group 1: Standard diet (STD) contained 3,000 kcal AMEn/kg energy and 23.78% CP; and Group 2: Reduced crude protein diet (RCP) contained 3,000 kcal AMEn/kg energy and 21.23% CP. From day 15-35 a common standard grower/finisher diet (3,150 kcal AMEn/kg energy and 22.18% CP) was allocated to both groups. Zymosan, a glycan derived from yeast cell walls that binds to TLR 2 and Dectin-1, was used for intra-abdominal challenge. Results demonstrated that a reduced crude protein starter diet (21.23 vs. 23.78% CP) between age 1-14, while maintaining the same levels of metabolizable energy and essential amino acids, did not affect broilers growth performance or lymphoid organ weights (P > 0.05). Interestingly, basal leukocyte levels in the RCP group significantly (P < 0.01) increased in the blood compartment at d35 in the unchallenged birds. Significant enhancements to leukocyte infiltration into the abdominal cavity were also detected post-immune challenge with zymosan (day 14 and day 35; P < 0.01). Post-challenge levels of TNF-α, IL-1β, and CXCL8 gene expression cells collected from the abdominal cavity were not affected by the diets (P > 0.05). Moreover, dietary treatments did not influence percentage of ROS producing cells in the abdominal cavity (P > 0.05). To our best knowledge, this is the first study that reports the impacts of reduced crude protein diet on the innate immune response of poultry to an acute inflammation model in the abdominal cavity. Overall, our results highlight that reduced crude protein diets can be used without negatively impacting broiler performance and may enhance the capacity of broilers to recruit leukocytes upon infection.

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Section: Methodsmentioning

confidence: 99%

“…Gene expression was performed as previously described (17,20 were determined by using a NanoDrop spectrophotometer and 2100 Bioanalyzer. The data were normalized to chicken from time 0 h. The ribosomal RNA 28S gene was used as an internal and normalization.…”

Section: Gene Expressionmentioning

confidence: 99%

Impact of Reduced Dietary Crude Protein in the Starter Phase on Immune Development and Response of Broilers Throughout the Growth Period

Kamely

Wakaruk

et al. 2020

Front. Vet. Sci.

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“…We reported adverse effects of caffeine on blood biochemistry and thyroid hormones (Kamely et al, 2016a), as well as right ventricular failure occurrence in chicks that were susceptible to pulmonary hypertension (Kamely et al, 2016b). We also reported that PDE5, troponin T2, ADORA1 and SERT gene expression increases in chickens suffering from pulmonary hypertension, right ventricular failure and heart failure (Kamely et al, 2019). This paper reports the effect of long‐term low to high dosage of caffeine intake on cardiovascular parameters.…”

Section: Introductionmentioning

confidence: 82%

“…Gene expression was performed as previously described (Kamely et al, 2019). In brief, total RNA was isolated from each chick's heart and reverse‐transcribed into cDNA.…”

Section: Methodsmentioning

confidence: 99%

Impacts of caffeine on resistant chicken's performance and cardiovascular gene expression

Kamely

Torshizi

West

et al. 2021

Animal Physiology Nutrition

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We previously reported a study on 288 broiler (Gallus gallus) chicks who received caffeine in water between days 3 and 42, at levels of 0, 6.25, 12.5, 25, 50 and 100 mg/kg body weight (BW)/day. In the previous report, we found that caffeine caused pulmonary hypertension (PH)‐associated mortality in a significant minority (20%–30%) of birds, including right ventricular hypertrophy and ascites. We have also shown a significant upregulation of the serotonin transporter (SERT), troponin T2, adenosine A1 receptor (ADORA1) and phosphodiesterase 5A (PDE5) in chicken suffering from PH. Here, we examine the resistant (survived) chicks from the first study that had not died due to acute heart failure and did not have clinical signs of pulmonary hypertension. Our goal was to determine whether birds who lacked overt signs of disease had subclinical manifestations, including similar changes in gene expression, growth rates and altered systemic haemodynamics. We found that growth was significantly increased by caffeine consumption (p < 0.01) at low doses; however, dosage over 50 mg/BW/d had remarkable adverse effects on growth (p < 0.01). Blood pressure, troponin T2 and PDE5 gene expression were not significantly altered by caffeine administration (p > 0.05). However, SERT gene expression linearly increased with increasing caffeine dosage (p < 0.01). The impact of caffeine on ADORA1 gene expression was dose dependent and nonlinear. In conclusion, despite the significant effects of caffeine on birds’ growth, no significant negative effects of caffeine were observed on the cardiovascular function of resistant chickens. This work provides valuable information for further study on different dosage of caffeine in an animal model.

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“…These proteins contribute to tropomyosin-troponin binding (troponin-T), actinmyosin contraction by regulating actin and myosin (troponin-I), or by binding calcium in the troponin structure by inhibiting actomyosin ATPase activity (O'Brien et al, 2006;Kamely et al, 2019). Circulating cTnI has recently been recognized as a biomarker by the American College of Cardiology for the diagnosis of cardiomyocyte damage and acute coronary syndromes (O'Brien, 2008;Kusumoto et al, 2012;Sato et al, 2012;Kamely et al, 2019). The amount of cardiac troponin in all organisms depends on the type, duration, and degree of myocyte injury (Alpert et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effects of Varying Essential Oil Mixture Concentrations Applied Underconditions of Different Temperature Stress on Cardiac Markers and Other Blood Parameters

Bayraktar

Tekce

2019

Braz. J. Poult. Sci.

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Cardiac troponin (cTnI) and erythrocyte distribution width (RDW) have been used as specific markers for the evaluation of cardiac arrhythmias and myocardial diseases. In this experimental study, we aimed to determine the dose-dependent administration of an Essential OilMixture (EOM) (Eucalyptus globulusLabill, Thymus vulgaris, Cymbopogon nardus, and Syzygiumaromaticum) on the serum erythrocyte indices and myocardial damage. We used 400 male, oneday-old, Ross-308 chicks. Each group ((n=8) (22°C Control (C), C+250 mL/1,000 L, C+500 mL/1,000 L, C+750 mL/1,000 L), 36°C (stress control (SC), SC+250 mL/1,000 L, SC+500 mL/1,000 L, SC+750 mL/1,000 L) was further divided into eight groups with 50 animals. Each group of 50 chicks was further subdivided into five groups with 10 animals in each compartment. The results showed that the WBC, RBC, HGB, HCT, RDW-SD, RDW-CV, and Lymphocyte decreased in groups without exposure to temperature stress (22ºC) compared to the 22ºC control group; whereas, CnTnI, MCHC, NEUT, and IG (p<0.05) increased compared to the control group. In groups exposed to temperature stress (36ºC), the WBC, HGB, HCT, RDW-SD, and Lymphocyte decreased compared to the control group, but MCH, MCHC, NEUT, and IG increased compared to the control. However, cTnI, CK, creatinine, RBC, and RDW had no effect on CD, MONO, EO, and BASO (p<0.05). EOM mixture had no effect on hematological and biochemical parameters.

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Upregulation of SERT and ADORA1 in broilers with acute right ventricular failure

Cited by 5 publications

References 37 publications

Impact of Reduced Dietary Crude Protein in the Starter Phase on Immune Development and Response of Broilers Throughout the Growth Period

Impact of Reduced Dietary Crude Protein in the Starter Phase on Immune Development and Response of Broilers Throughout the Growth Period

Impacts of caffeine on resistant chicken's performance and cardiovascular gene expression

Effects of Varying Essential Oil Mixture Concentrations Applied Underconditions of Different Temperature Stress on Cardiac Markers and Other Blood Parameters

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