Uptake and Assimilation of Hydrophobic Substrates by the Oleaginous Yeast Yarrowia lipolytica

Thevenieau, France; Béopoulos, Athanasios; Desfougères, Thomas; Sabirova, Julia; Albertin, K.; Zinjarde, Smita; Nicaud, Jean‐Marc

doi:10.1007/978-3-540-77587-4_104

Cited by 44 publications

(41 citation statements)

References 26 publications

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“…The assimilation and metabolism of hydrophobic materials in Y. lipolytica has been previously reviewed (Fickers et al, 2005a;Thevenieau et al, 2010;Fukuda, 2013). However, the transport mechanisms of these substrates are not clear.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Liu

Huang

2015

Biotechnology Advances

212

115

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Section: Accepted Manuscriptmentioning

confidence: 99%

Biotechnological applications of Yarrowia lipolytica: Past, present and future

Liu

Huang

2015

Biotechnology Advances

212

115

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“…Together with glycolysis, it can contribute significantly to the energy supply of resting cells [115]. At the stationary phase, a higher expression of cytochrome P450 monoxygenases [104] and lipid remobilization [40] are also expected. These features enable the large-scale application of engineered Y. lipolytica cells without the need for cofactor addition.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Engineering of Yarrowia lipolytica as a Promising Cell Biorefinery Platform for the de novo Production of Multi-Purpose Long Chain Dicarboxylic Acids

2017

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This proof-of-concept study establishes Yarrowia lipolytica (Y. lipolytica) as a whole cell factory for the de novo production of long chain dicarboxylic acid (LCDCA-16 and 18) using glycerol as the sole source of carbon. Modification of the fatty acid metabolism pathway enabled creating a pool of fatty acids in a β-oxidation deficient strain. We then selectively upregulated the native fatty acid ω-oxidation pathway for the enhanced terminal oxidation of the endogenous fatty acid precursors. Nitrogen-limiting conditions and leucine supplementation were employed to induce fatty acid biosynthesis in an engineered Leu -modified strain. Our genetic engineering strategy allowed a minimum production of 330 mg/L LCDCAs in shake flask. Scale up to a 1-L bioreactor increased the titer to 3.49 g/L. Our engineered yeast also produced citric acid as a major by-product at a titer of 39.2 g/L. These results provide basis for developing Y. lipolytica as a safe biorefinery platform for the sustainable production of high-value LCDCAs from non-oily feedstock.

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“…The yeast is commonly isolated from natural environments such as oil fields, foods, sewagetreatment plants, and oil-contaminated sites [21]. Y. lipolytica can attach to large lipid droplets (due to its hydrophobic cell surfaces) and secrete both surfactants and emulsifiers [22,23]. Lipids are often encountered in the form of ester conjugates, mainly as TAGs, which must be broken down into their components (i.e., fatty acids and glycerol) by extracellular lipases to be incorporated into cells [23].…”

Section: Hydrophobic Substratesmentioning

confidence: 99%