Uptake and intracellular distribution of various metal ions in human monocyte-derived dendritic cells detected by Newport Green™ DCF diacetate ester

Cadosch, Dieter; Meagher, James F.; Gautschi, Oliver; Filgueira, Luis

doi:10.1016/j.jneumeth.2008.12.008

Cited by 18 publications

(11 citation statements)

References 39 publications

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“…At this stage, it would be logical to deduce that such cellular uptake of soluble zinc induced at least in part the observable lethal cellular damage. Due to its high affinity for soft Lewis bases donor group of the Cys (‐(S − ), pKa = 8.18) and His (‐(NH + ), pKa = 6) side chains, the detected excess of cellular zinc may form complexes with structural and catalytic proteins (Newport Green DCF also probes zinc‐protein complexes) . Such non‐specific zinc‐protein complexation has been indicated to result in the inhibition of vital enzymes, such as NADH oxidase and glutathione reductase .…”

Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Gunawan

Teoh

ricardo

et al. 2013

Part & Part Syst Charact

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Zinc oxide nanoparticles (ZnO NPs) induce morphological transformation of Escherichia coli from its native rod‐shape of ≈2–4 μm to filamentous cells of 20–40 μm in length. The transient response can only be observed at up to 3.5 h proliferation, beyond which the cytotoxic effect is neutralized and the rod‐shape is restored. The filamentation is part of the bacterium SOS response to the Trojan horse‐type internalization of undissolved ZnO solids. In the absence of ZnO solids, no cell filamentation can be observed from the leached soluble zinc fraction or dissolved zinc salt.

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Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Gunawan

Teoh

ricardo

et al. 2013

Part & Part Syst Charact

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“…To investigate the ability of OC to corrode titanium and take up corresponding ions, paraformaldehyde (1% in PBS) fixed cells adherent to the metal foils and glass coverslips were incubated for 1 h at room temperature with Newport Green DCF diacetate ester at 1 μ M concentration (Molecular Probes/Invitrogen) and washed with PBS 10. Subsequently, the cells were stained with DAPI nuclear stain and mounted in Dako Fluorescent Mounting Medium (DakoCytomation) for confocal microscopy.…”

Section: Methodsmentioning

confidence: 99%

“…However, in the presence of certain anions found in biological fluids, such as phosphates, titanium forms complexes with lower water solubility, resulting in concentrations below detectable levels. Thus, we have recently established a fluorescent method for the detection of complex, intracellular metal ions, including titanium, using Newport Green DCF diacetate ester 10…”

Section: Introductionmentioning

confidence: 99%

“…Thus, we have recently established a fluorescent method for the detection of complex, intracellular metal ions, including titanium, using Newport Green DCF diacetate ester. 10 OC are highly specialized, often multinucleated cells, which are uniquely capable of bone resorption. 11 They differentiate from marrow-derived mononuclear precursors, which circulate in the CD14 þ monocyte fraction of peripheral blood, in the presence of macrophage-colony stimulating factor (M-CSF) and the receptor activator of NF-jB ligand (RANK-L), which is expressed by osteoblasts and other bone-related stromal cells.…”

Section: Introductionmentioning

confidence: 99%

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Biocorrosion and uptake of titanium by human osteoclasts

Cadosch

Al-Mushaiqri

Gautschi

et al. 2010

J Biomedical Materials Res

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All metals in contact with a biological system undergo corrosion through an electrochemical redox reaction. This study investigated whether human osteoclasts (OC) are able to grow on titanium and aluminum, and directly corrode the metals leading to the release of corresponding metal ions, which are believed to cause inflammatory reactions and activate osteoclastic differentiation. Scanning electron microscopy analysis demonstrated long-term viable OC cultures on the surface of titanium and aluminum foils. Atomic emission spectrometry investigations showed significantly increased levels of aluminum in the supernatant of OC cultured on aluminum; however, all measurements in the supernatants of cell cultures on titanium were below detection limits. Despite this, confocal microscopy analysis with Newport Green DCF diacetate ester staining depicted intense fluorescence throughout the cytoplasm and nucleolus of OC cultured on titanium foils. Comparable fluorescence intensities were not observed in monocytes and control cells cultured on glass. The present study demonstrated that human osteoclast precursors are able to grow and differentiate toward mature OC on titanium and aluminum. Furthermore, it established that the mature cells are able to directly corrode the metal surface and take up corresponding metal ions, which subsequently may be released and thereby induce the formation of osteolytic lesions in the periprosthetic bone, contributing to the loosening of the implant.

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“…NPG is readily taken up by cells and distributed in the cytoplasm where metals such as Zn are expected to pool [23, 33]. In this work NPG was titrated with these two biologically relevant transition metals Ni and Zn and studied using optical spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy

et al. 2018

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In this work we demonstrate that time domain techniques can be used successfully to monitor realtively weak modulations of the fluorescence in sensing applications. The metal sensing complex Newport Green DCF™ can detect selected transition metals in vivo as well as in vitro. Incremental addition of Ni and/or Zn (in vitro) lead to a substantial reduction in the yield of the fast component in a bi-exponential fluorescence decay (τ1 = 150–250 ps) from 60% to 30–35%. This is rationalised as an inhibition of intra-molecular electron transfer in the NPG sensing complex due to metal complexation. In order to explore this effect in cellulo, NIH 3 T3 mouse skin fibroplast cells were pre-incubated with set levels of Ni and Zn, at a constant concentration of NPG. The fluorescence modulation in cellullo was subsequently studied employing both time-resolved fluorescence microscopy and confocal fluorescence microscopy. In correlation with the in vitro observations, similar effects were observed on the fluorescence decay in cellulo.Electronic supplementary materialThe online version of this article (10.1007/s10895-018-2335-z) contains supplementary material, which is available to authorized users.

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Uptake and intracellular distribution of various metal ions in human monocyte-derived dendritic cells detected by Newport Green™ DCF diacetate ester

Cited by 18 publications

References 39 publications

Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Zinc Oxide Nanoparticles Induce Cell Filamentation in Escherichia coli

Biocorrosion and uptake of titanium by human osteoclasts

In Vitro and in Cellulo Sensing of Transition Metals Using Time-Resolved Fluorescence Spectroscopy and Microscopy

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