Uptake and metabolism of leaf proteins by the silverleaf whitefly

Salvucci, Michael E.; Rosell, Rosemarie C.; Brown, Judith K.

doi:10.1002/(sici)1520-6327(1998)39:4<155::aid-arch3>3.0.co;2-#

Cited by 30 publications

(11 citation statements)

References 33 publications

(39 reference statements)

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“…Likewise, while working with cotton, Bi et al (2001Bi et al ( , 2003 found that nitrogen fertilization also enhanced soluble protein levels in cotton petioles. In another study, Salvucci et al (1998) found that phloem-feeding whiteflies can readily ingest and assimilate cotton proteins. However, MSD (minimum significant difference) 5% N 9 days = 8.285…”

Section: Resultsmentioning

confidence: 99%

Modulating infestation rate of white fly (Bemicia tabaci) on okra (Hibiscus esculentus L.) by nitrogen application

et al. 2010

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Pests are major factor affecting the crop yield, quality, and esthetic value. Although pest resistance is genetically controlled, it is mediated through physiological and biochemical processes interrelated with the nutritional status of the plant. Nutrition of plant may determine its resistance or susceptibility to pests. An experiment was conducted to determine whether and up to what extent varying concentrations of N modulate the infesting rate of whitefly on Okra (Hibiscus esculentus). Twenty-one-dayold okra plants were subjected to five concentrations of 4, 8, 12, 16, and 20 mM N for 30 days. Number of eggs, nymph were counted on each plant after every 4 days. Growth and yield of okra plants increased with increasing supply of N but it decreased slightly at the highest level of N (20 mM). Leaf soluble proteins, amino acids, and soluble sugars were determined on fresh weight basis. From these results, it is clear that increasing N level increased the biosynthesis or accumulation of protein, free amino acid, and sugars that might have attracted more whitefly for feeding.

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Section: Resultsmentioning

confidence: 99%

Modulating infestation rate of white fly (Bemicia tabaci) on okra (Hibiscus esculentus L.) by nitrogen application

et al. 2010

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“…Assays were conducted in triplicate and the results presented are the means þ S.E.M. Cross-linked protein was analyzed by sodium dodecyl sulfate^polyacrylamide gel electrophoresis (SDS^PAGE) on 10% gels [8]. Fluorescence of NBD-modi¢ed activase was determined as described previously [13].…”

Section: Analysis Of Cross-linked and Chemically Modi¢ed Activasementioning

confidence: 99%

“…Various lines of evidence indicate that residues involved in the interaction with Rubisco are located outside the region of homology with other AAA þ proteins at both the C-and the N-terminus of activase. Thus far, e¡orts to derive a 3-D structure of Rubisco activase using crystallography have not been forthcoming, frustrated in all likelihood by the highly polydispersed nature of the protein [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Like other AAA þ proteins the active form of activase is a higher ordered oligomer with as many as 16 subunits in its most active form [3,9,10]. Since these subunits interact cooperatively for both ATP hydrolysis and Rubisco activation [8,9,11], the nature of these interactions is of interest for understanding activase function. Here we describe the results of directed mutagenesis, cross-linking and £uorescence experiments that examined the proximity of the N-and C-terminal regions of the protein and investigated their roles in activase function.…”

Section: Introductionmentioning

confidence: 99%

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Potential for interactions between the carboxy‐ and amino‐termini of Rubisco activase subunits

Salvucci

2004

FEBS Letters

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The subunit interactions of Rubisco activase were investigated using mutants containing an introduced Cys near the N-and/or C-terminus. Chemical cross-linking of the C-terminal and double insertion mutant produced subunit dimers and dimers plus high ordered oligomers, respectively. Fluorescence measurements with N,NP P-dimethyl-N-(iodoacetyl)-NP P-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine showed that the environment around the introduced Cys near the C-terminus becomes more hydrophilic upon nucleotide binding. The Cys insertion mutants catalyzed Rubisco activation and ATP hydrolysis even when the subunits of the C-terminal or double insertion mutants were completely cross-linked. The results indicate that the termini of adjacent activase subunits are in close proximity and can be modi¢ed and even joined without a¡ecting enzyme function.

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“…These piercing-sucking insects are sensitive to levels of free amino acids and proteins which they extract from phloem (Salvucci et al 1998, Crafts-Brandner 2002. Previous studies have found that whiteßies feed and oviposit more readily, or develop more quickly on plants with higher nitrogen Clip Cages.…”

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confidence: 99%

Effects of Selected Fertilizers on the Life History of Bemisia tabaci (Hemiptera: Aleyrodidae) Biotype B

et al. 2011

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We tested the effects among a purportedly sustainable water-soluble fertilizer, a conventional water-soluble fertilizer, an alternation of these, a controlled-release fertilizer, and a clear water control on the life-history traits of sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae; =Bemisia argentifolii Bellows & Perring) biotype B reared on poinsettia (Euphorbia pulcherrima Willdenow ex Klotzch). Free amino acids in petioles were measured to estimate plant nutrient assimilation and phloem nutritional quality for B. tabaci biotype B. The sustainable fertilizer produced plants with the highest concentration of amino acids. In contrast, fecundity of whiteflies was lowest in plants treated with the sustainable fertilizer and the water control. The relationship between total amino acids in phloem and survival was significantly quadratic, with the highest survival at intermediate levels. Fecundity, however, was negatively correlated with total amino acid content of the maternal host plant. Variation in total amino acid concentration in petioles of plants treated within fertilizer treatments makes it difficult to predict whether a particular fertilizer will produce plants with enough amino acids to deleteriously affect both survivorship and fecundity and yet yield a plant of good quality. Despite this limitation, we can conclude that the use of this sustainable fertilizer will not cause increases in whitefly populations relative to plants fertilized with water-soluble and slow-release fertilizers that deliver the same level of nitrogen to the plant.

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Uptake and metabolism of leaf proteins by the silverleaf whitefly

Cited by 30 publications

References 33 publications

Modulating infestation rate of white fly (Bemicia tabaci) on okra (Hibiscus esculentus L.) by nitrogen application

Modulating infestation rate of white fly (Bemicia tabaci) on okra (Hibiscus esculentus L.) by nitrogen application

Potential for interactions between the carboxy‐ and amino‐termini of Rubisco activase subunits

Effects of Selected Fertilizers on the Life History of Bemisia tabaci (Hemiptera: Aleyrodidae) Biotype B

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