Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells

Abstract

In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 microns in diameter composed of 50:50 lactic acid:glycolic acid were instilled into intestinal segments containing jejunal or ileal Peyer's patches, and uptake by M cells was examined by electron microscopy. PLGA microspheres visualized as electron-lucent, spherical particles were taken up by M cells by pseudopod-like extensions of the M cell apical membrane and translocated to the pocket region containing mononuclear leukocytes within 60 min. These results indicate that PLGA microspheres can be directed to M cell apical surfaces for delivery to immunocompetent cells in gut-associated lymphoid tissues.

Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue

“…particulate/macromolecular antigen uptake and transcytosis, a characteristic that has been defined both in vivo and in vitro by using microparticle-uptake assays (Porta et al 1992;Ermak et al 1995;Pappo and Ermak 1989;Landsverik 1988). In this study, a functional assessment of M-cells has been carried out in tissue explants from terminal rectal mucosa and by in vivo calf challenges.…”

Collection and Processing of External Secretions and Tissues of Mouse Origin

“…These extensions, which almost totally surround B and T lymphocytes and occasionally MØs as well, form what appears to be a pocket (Gebert et al, 1996;Neutra et al, 1996;Owen and Jones, 1974). The M cells, which have short microvilli, small cytoplasmic vesicles, and few lysosomes, are involved in the uptake and transport of lumenal Ags, including proteins and particles such as viruses, bacteria, small parasites, and microspheres (Ermak et al, 1995;Gebert et al, 1996;Neutra et al, 1996;Wolf and Bye, 1984 Carefully pull out the small intestine by clipping off the mesentery until all of the small intestine is exposed. 5.…”

“…However, they were later renamed M cells because of their unique topical morphology (microfold/membranous) (Owen and Jones, 1974) and because the entire epithelium covering the MALT is now recognized as being of an FAE type (Farstad et al, 1994;Gebert et al, 1996;Neutra et al, 1996;Wolf and Bye, 1984). The surface of the dome region is covered by the specialized FAE, up to 10-20% of which is M cells that exhibit thin extensions around lymphoid cells (Bockman and Cooper, 1973;Ermak et al, 1995;Farstad et al, 1994;Gebert et al, 1996;Neutra et al, 1996;Owen and Jones, 1974;Wolf and Bye, 1984). These extensions, which almost totally surround B and T lymphocytes and occasionally MØs as well, form what appears to be a pocket (Gebert et al, 1996;Neutra et al, 1996;Owen and Jones, 1974).…”

Oral Protein and Peptide Drug Delivery

“…This approach is supported by the literature, which states that particles in the nanosize range are absorbed intact by the intestinal epithelium, especially Peyer's patches, and travel to sites such as the liver, the spleen, and other tissues. [40][41][42] The proteins and peptides encapsulated in the nanoparticles are less sensitive to enzyme degradation through their association with polymers. The extended release of protein and peptide drug from the particles could have pharmacological and clinical significance.…”