Uptake of colostral leukocytes in the intestinal tract of newborn calves

Liebler-Tenorio, Elisabeth M.; Riedel-Caspari, G; Pohlenz, Joachim

doi:10.1016/s0165-2427(01)00404-4

Cited by 63 publications

(52 citation statements)

References 13 publications

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“…Macrophages are the predominant cells in bovine colostrum and have potential for absorption by the intestinal mucosa of newborn calves when this food is offered as the fresh way in the first 12 h postpartum (Gomes et al, 2011;Liebler-Tenorio et al, 2002). Besides enhancing the phagocytic response of calves, the colostral macrophages may be the key to the activation and maturation of their specific immune response by increasing the capacity of monocytes to present antigens .…”

Section: Discussionmentioning

confidence: 99%

Development of the Innate Immune Response and Influence of Colostrum Suckling in Calves

Gomes

Baccili

Alvim

et al. 2014

American Journal of Animal and Veterinary Sciences

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Bovine colostrum contains immune factors, like leukocytes and cytokines that are absorbed by the intestinal mucosa of the neonate, however, not much is known about the function of some of these components in the active immune response of calves. The aim of this study was to determine the influence of colostrum intake and development of the innate immune response of calves from birth to two months of age, by evaluating the proportion of neutrophils CH138 + and monocytes CD14 + , using flow citometry and measurement of proinflammatory cytokines IL-1 beta, IL-6, TNF-alpha and IFN-gamma, using comercial ELISA kits. According to the obtained results we can determine that colostrum intake had influenced the IFN-gamma concentration and the monocytes CD14 + and neutrophils CH138 + proportions in the circulation of calves and that these components seem to play a key role in the development of immune response of these animals, providing protection against pathogens, enhancing the phagocytic activity and antigen presentation by monocytes.

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Section: Discussionmentioning

confidence: 99%

Development of the Innate Immune Response and Influence of Colostrum Suckling in Calves

Gomes

Baccili

Alvim

et al. 2014

American Journal of Animal and Veterinary Sciences

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“…Bovine colostrum typically contains between 1x10 6 and 2.5 x10 6 somatic cells/mL (Liebler-Tenorio et al 2002, Gomes et al 2011. About 30% of these cells are viable (Godden et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Maternal cells have been detected in the circulation, Peyer's patches and a variety of lymph nodes of neonatal calves after 24 to 96 hours relative to ingestion of colostrum (Aldridge et al 1998, Liebler-Tenorio et al 2002, Reber et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Specific immune response in neonate Holstein heifer calves fed fresh or frozen colostrum

et al. 2017

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The objective of this study was to evaluate the influence of viable cells from colostrum on immune development in dairy heifer calves during the first 28 days of life. The animals were distributed between 2 groups: COL+ (n=9) receiving fresh whole colostrum from their own damns; and COL-(n=10) receiving pooled frozen colostrum, containing no viable cells, from a pool of donor cows. These calves were assessed before colostrum intake (D0), 48 hours of age (D2), and weekly from D7 to D28. The development of immunity was evaluated by assessment of the phenotype of blood leukocyte subsets, and induced cytokine production after 72 hours of stimulation in culture with concanavalin A (ConA), killed Staphylococcus aureus (S.aureus) and killed Escherichia coli (E. coli) by peripheral blood mononuclear cell (PBMC). The clinical history of these calves was marked by a high frequency of diarrhea in both groups. However, COL-had greater diarrhea intensity scores (fecal score~3 of 4), and rectal temperature on D7 than COL+ calves. Moreover, bronchopneumonia (n=1) and navel inflammation were observed only in COL-calves. COL-had a lower concentration of serum iron, and a higher absolute number of lymphocytes on D7 than COL+. COL-also had a higher percentage of anemic calves than the COL+ calves on D21 and D28. In general, the percent of cells within each subset of leukocytes was similar between the groups over the experiment, except on week 1 when COL-calves had a higher percentage of lymphocytes expressing CD45RO + (P=0.07). A steady increase in CD45RO + and concomitant decline in CD45RO -leukocytes was observed over the course of the study, indicating the development of immune memory. The proportion of CD14MHCII + leukocytes increased with age (P≤0.05). The median background cytokine production by PBMC that were not stimulated was below the level of detection of the assays used for both groups. The PBMC from COL+ calves stimulated with ConA secreted a larger quantity of IL-17 week 2 (COL+=2060.0pg/mL and COL-=0.0pg/mL, P=0.00). PBMC from COL+ calves stimulated with killed S. aureus whole cell antigen (P=0.05) and killed E. coli whole cell antigen (P=0.05) also secreted higher levels of IL17 than COL-calves at week 4. Clear production of IL17 was observed in PBML from COL+ calves at week 2, but the difference was not statistical different between groups. In conclusion, calves fed fresh and frozen colostrum showed no difference in cells subset profile overall. The increased percentage of leukocytes expressing the memory CD45RO + or CD14MHCII + over the course of the experiment indicated a maturation of the adaptive immune response after natural exposure to pathogens in the + aumentou de acordo com a idade (P≤0.05). As medianas das citocinas produzidas a partir do PBMC não estimuladas apresentaram valores abaixo do nível de detecção em ambos os grupos. O PBMC do COL+ estimulado com ConA secretou elevada concentração de IL17 na semana 2 (COL+=2060.0pg/mL e COL-=0.0pg/ml, P=0.00). PBMC do COL+ estimuladas com S. aureus inativado (P...

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“…Bovine colostral leukocytes (CL) are mainly composed of neutrophils, macrophages, and lymphocytes (Duhamel et al, 1987). Uptake of these CL through the intestinal barrier has been shown in cattle, with a preferential route of uptake through the follicle-associated epithelium of Peyer's patches (Liebler-Tenorio et al, 2002). The functional importance of bovine CL is not yet fully understood, but they seem to enhance lymphocyte responses (Reber et al, 2005;Donovan et al, 2007), increase phagocytosis and bacterial-killing ability (Riedel-Caspari and Schmidt, 1991), and stimulate humoral immune responses in the neonatal calf (Riedel-Caspari, 1993).…”

Section: Introductionmentioning

confidence: 99%

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

Meganck

Goddeeris

Campeneere³

et al. 2015

Journal of Dairy Science

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In neonatal calves, the ingestion of colostrum is imperative for preventing infectious diseases. Investigations into the transfer of passive immunity of cattle have primarily focused on the importance of colostral immunoglobulins, with a recent increase in focus on understanding the role of colostral leukocytes. The main objective of the present study was to measure the influence of parity, body condition score, serum nonesterified fatty acids, and serum β-hydroxybutyrate concentrations of periparturient cows on phenotype and mitogen-and antigen-induced proliferative capacity of bovine colostral leukocytes. Holstein-Friesian cows (n = 141) were intramuscularly vaccinated at 60 and 30 d before the expected parturition date with a tetanus toxoid vaccine. Of these 141 animals, 28 primiparous and 72 multiparous cows were sampled immediately after parturition. Colostrum mononuclear cell populations were identified by flow cytometry using bovine cluster of differentiation markers, and the proliferative capacity of these cells was determined using a 3 H-thymidine proliferation assay. Under-conditioned cows had a significantly higher percentage of colostral macrophages than normal-conditioned animals, whereas over-conditioned cows had significantly more colostral B-lymphocytes. Serum β-hydroxybutyrate was significantly associated with higher numbers of colostral T-lymphocytes and macrophages. Heifers had significantly higher mitogen-and antigen-induced proliferation of their colostral leukocytes than third parity or older cows. In conclusion, body condition score, parity, and serum β-hydroxybutyrate concentration of periparturient high-yielding dairy cows were shown to influence the number of colostral macrophages or the mitogen-and antigen-induced proliferation of colostral leukocytes, possibly influencing the cellular immunity of the newborn calf.

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Uptake of colostral leukocytes in the intestinal tract of newborn calves

Cited by 63 publications

References 13 publications

Development of the Innate Immune Response and Influence of Colostrum Suckling in Calves

Development of the Innate Immune Response and Influence of Colostrum Suckling in Calves

Specific immune response in neonate Holstein heifer calves fed fresh or frozen colostrum

Effect of β-hydroxybutyric acid, parity, and body condition score on phenotype and proliferative capacity of colostral mononuclear leukocytes of high-yielding dairy cows

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