Uptake of exogenous DNA by mouse embryos

Ledoux, L; Charles, P

doi:10.1016/0014-4827(67)90200-5

Cited by 20 publications

(3 citation statements)

References 5 publications

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“…This polymerase might enter the animal cells with the bacterial DNA or be synthesized subsequently. This latter possibility seems less likely since, in our experimental conditions, purified, extracted bacterial DNA is not transcribed in animals although it enters the cells (8,14).…”

Section: Discussionmentioning

confidence: 98%

Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Stroun

Anker

1973

J Bacteriol

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After frog auricles have been in contact with a suspension of bacteria or bacteria-free supematant fluid, newly synthesized bacterial ribonucleic acid (RNA) is recovered in animal cells. It appears that the presence of bacterial deoxyribonucleic acid (DNA)-dependent RNA polymerase is necessary for the transcription of bacterial DNA in the host cells. This phenomenon seems to be related to a transfer of DNA and DNA-dependent RNA polymerase from bacteria into animal cells.

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Section: Discussionmentioning

confidence: 98%

Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Stroun

Anker

1973

J Bacteriol

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“…However, the function of any released DNA would be questionable because suppression in our system has an absolute requirement for cell contact. The uptake of heterologous DNA has been observed with mammalian tissue in vivo (17) and with cells cultured in vitro (18)(19)(20). In addition, RNA extracted from normal tissue has been reported to exert suppressive effects evidenced mainly by enhanced skin graft survival (21)(22)(23)(24) and depressed serum anti-SRBC titers (25,26 …”

Section: Discussionmentioning

confidence: 99%

Leukemia in AKR mice: A defined suppressor cell population expressing membrane-associated DNA

Russell¹,

Golub²

1978

Proc. Natl. Acad. Sci. U.S.A.

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AKR mice develop a spontaneous thymic leukemia after 8 months of age (1). We have previously shown that thymus and spleen cells from these leukemic animals are capable of suppressing the in vitro generation of an antibody response (2) by normal AKR spleen cells. This suppression requires cell contact (2, 3), has unique genetic restrictions (4), and is usually (but not always) susceptible to x-irradiation and mitomycin treatment (4). The susceptibility to x-irradiation and mitomycin treatment suggests involvement of DNA, and cell separation studies enforced this idea because the cells with the highest endogenous rate of DNA synthesis cosediment with the fraction of cells that carry out suppression (5). The need for cell contact led us to investigate the ability of isolated membranes to suppress normal responses; membranes from leukemic cells are able to suppress the responses of normal cells (unpublished results).In an effort to reconcile the apparent involvement of DNA, the need for cell contact, and the ability of isolated membranes to suppress (unpublished data), we tested one of the several possible explanations-namely, that the leukemic suppressor cell has a form of DNA on its surface that is somehow involved in the suppression. In this paper we show that the suppression of in vitro anti-sheep erythrocyte (SRBC) responses by leukemic cells is susceptible to DNase treatment and the suppressing cells can be removed after being reacted with anti-DNA antibodies and passed through an immunoadsorbant column. Preparation and Use of Cell Affinity Chromatography Columns. Goat anti-rabbit IgG (IgG fraction purified by DEAE-cellulose chromatography) dissolved in 0.1 M borate, pH 8/0.5 M NaCI (coupling buffer) was coupled to CNBractivated Sepharose 6MB (10 mg of antibody per ml of swollen gel) on an end-over-end shaker at room temperature for 2 hr. Any remaining reactive groups on the gel were blocked by incubation of the gel in 1 M ethanolamine at pH 8 for 2 hr. Blocking agent and noncovalently adsorbed protein were removed by washing in 0.1 M acetate, pH 4/0.5 M NaCI followed by coupling buffer. MATERIALS AND METHODSFor columns 12-ml disposable plastic syringes were fitted with 80-am pore size plastic discs (Bellco Glass) and three-way stopcocks (Pharmaseal, Puerto Rico). Columns were loaded with 8 ml of the Sepharose-antibody conjugate, washed, and stored with coupling buffer containing 0.1% NaN3. Before use, columns were equilibrated with RPM!1 medium containing 5% fetal calf serum and 0.025 M EDTA (RPMI/EDTA) for 30 min at 4VC. Cell suspensions treated with antibody (see antisera treatment) were washed three times in RPMI/EDTA and were suspended at 2-3 X 108 cells per 1.5 ml of RPMI/EDTA. The cells were gently layered onto the column with a sterile pasteur pipette and washed into the column with RPMI/EDTA. Cells were allowed to react on the column for 45 min at 4VC. Nonbound cells (fraction I) were collected in RPMI/EDTA at 0.5 ml/min for the first 15 ml; then an additional 30 ml were collected at a rapid flow rate...

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“…The conclusion was that the embryo did not utilize nucleosides or nucleotides from the uterine deciduum for its DNA synthesis but that the trophoblast incorporated label in the form of macromolecular DNA. Ledoux and Charles (1967) also demonstrated the uptake by trophoblast of exogenous DNA injected into pregnant mice.…”

Section: Discussionmentioning

confidence: 93%

An Autoradiographic Study of the Implantation of Transferred Mouse Blastocysts

Kaye¹

1977

Aust. Jnl. Of Bio. Sci.

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Mouse blastocysts collected on day 4 were cultured in [3Hlthymidine (0·01 .uCi/ml) for 24 hand transferred to the uteri of pseudopregnant recipients. Autoradiography revealed that when such blastocysts were allowed to develop for 48 h in utero, label was apparent in the nuclei of decidual cells. The experimental conditions were physiological since blastocysts developed into normal offspring when gestation was allowed to proceed in pseudopregnant recipient animals. The transfer of foetal DNA into maternal decidual cells may be of important immunological significance.

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Uptake of exogenous DNA by mouse embryos

Cited by 20 publications

References 5 publications

Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Transcription of Spontaneously Released Bacterial Deoxyribonucleic Acid in Frog Auricles

Leukemia in AKR mice: A defined suppressor cell population expressing membrane-associated DNA

An Autoradiographic Study of the Implantation of Transferred Mouse Blastocysts

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