Uptake of lead, cadmium and zinc by a novel bacterial exopolysaccharide

Loaëc, M.; Olier, R.; Guézennec, Jean

doi:10.1016/s0043-1354(96)00375-2

Cited by 198 publications

(107 citation statements)

References 25 publications

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“…These polymers are composed of sugars, amino acids and uronic acids (Bitton, 1994). Adsorptive properties of exopolymers have been well documented especially in terms of biosorption of pollutants and toxics (Beech and Cheung, 1995;Loaëc et al, 1997). However, the role of EPS in biological phosphorus removal has not been well studied.…”

Section: Introductionmentioning

confidence: 99%

The role of extracellular exopolymers in the removal of phosphorus from activated sludge

2001

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Section: Introductionmentioning

confidence: 99%

The role of extracellular exopolymers in the removal of phosphorus from activated sludge

2001

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“…Notably, marine bacterial exopolymers are recognized for their high uronic acid content (36), which is comparably higher than that found in exopolymers derived from marine planktonic algae (8) and nonmarine bacteria (22). This endows these macromolecules with a polyanionic nature-a property that has been proposed to enable these polymers to complex cationic species (38,39,68). Based on evidence from field studies in polar marine environments showing a correlation between enhanced phytoplankton activity and high levels of bacterial exopolymeric substances (2), it has been suggested that these polymers may play a role in the binding of trace metals, like iron, possibly leading to an increase in their availability to phytoplankton (3,8,45).…”

Section: Discussionmentioning

confidence: 99%

Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced byPseudoalteromonassp. Strain TG12

Gutiérrez¹,

Shimmield²,

Haidon³

et al. 2008

Appl Environ Microbiol

103

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In this study, we describe the isolation and characterization of a new exopolymer that exhibits high emulsifying activities against a range of oil substrates and demonstrates a differential capacity to desorb various mono-, di-, and trivalent metal species from marine sediment under nonionic and seawater ionicstrength conditions. This polymer, PE12, was produced by a new isolate, Pseudoalteromonas sp. strain TG12 (accession number EF685033), during growth in a modified Zobell's 2216 medium amended with 1% glucose. Chemical and chromatographic analysis showed it to be a high-molecular-mass (>2,000 kDa) glycoprotein composed of carbohydrate (32.3%) and protein (8.2%). PE12 was notable in that it contained xylose as the major sugar component at unusually high levels (27.7%) not previously reported for a Pseudoalteromonas exopolymer. The polymer was shown to desorb various metal species from marine sediment-a function putatively conferred by its high content of uronic acids (28.7%). Seawater ionic strength (simulated using 0.6 M NaCl), however, caused a significant reduction in PE12's ability to desorb the sediment-adsorbed metals. These results demonstrate the importance of electrolytes, a physical parameter intrinsic of seawater, in influencing the interaction of microbial exopolymers with metal ions. In summary, PE12 may represent a new class of Pseudoalteromonas exopolymer with a potential for use in biotechnological applications as an emulsifying or metal-chelating agent. In addition to the biotechnological potential of these findings, the ecological aspects of this and related bacterial exopolymers in marine environments are also discussed.

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“…Di samping itu, menurut Muraleedharan & Venkobachar (1990), perlakuan dengan perendaman dengan larutan alkali akan menghancurkan enzim autolitik yang menyebabkan pembusukan (putrefaction) biomassa dan menghilangkan lipid dan protein yang menyelubungi sisi aktif pengikatan logam. Perlakuan ini juga dapat melepaskan polimer polisakarida yang memiliki afinitas yang tinggi terhadap ion logam tertentu (Loaec, 1997). Perendaman biomassa dengan larutan NaOH terbukti telah meningkatkan kapasitas penyerapan Zn 2+ pada Aspergillus niger (Luef et al, 2004) pada permukaan sel melalui reaksi browning non-enzimatik akibat pemanasan (Loaec, 1997).…”

Section: Modifikasi Biomassa S Cerevisiaeunclassified

“…Perlakuan ini juga dapat melepaskan polimer polisakarida yang memiliki afinitas yang tinggi terhadap ion logam tertentu (Loaec, 1997). Perendaman biomassa dengan larutan NaOH terbukti telah meningkatkan kapasitas penyerapan Zn 2+ pada Aspergillus niger (Luef et al, 2004) pada permukaan sel melalui reaksi browning non-enzimatik akibat pemanasan (Loaec, 1997). Pemanasan ini juga berfungsi untuk mematikan S. cerevisiae.…”

Section: Modifikasi Biomassa S Cerevisiaeunclassified

Biosorpsi logam Zn oleh biomassa Saccharomyces cerevisiae *) Biosorption of Zn metal by Saccharomyces cerevisiae biomass

KRESNAWATY¹,

TRI-PANJI²

2016

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SummaryHeavy metal in waste water potentiallycauses environmental pollution. Generally,heavy metal pollutions come from metalplating, textile, latex-rubber goods, and otherindustries. The process of latex-rubber goodindustries uses heavy metal in the form ofZnO as accelerator for rubber vulcanizationprocess, so that Zn 2+ ion exists in wastewatereffluents in concentration as much as 300 ppm,whereas the maximum limit allowed is 2.5 ppm.The chemical way generally used to decreaseZn 2+ concentration in wastewater effluents isby adding bases, NaOH or Ca(OH) 2 until pHreached 11, hence this metal is precipitated asits hydroxide. However, the way is done, isvery high cost and has a risk of the emergencesecondary pollution caused by excess base. Analternative way to absorb Zn 2+ consideredinexpensive is by using biosorbent in the formof Saccharomyces cerevisiae biomass frombioethanol industrial waste. The research wasconducted using artificial wastewater withZn 2+ ion concentration of 300 ppm and the pHwas adjusted to the range between 3-7.Biosorption was conducted by addition of freeS. cerevisiae biomass as well as byimmobilized cells on filter paper. Observationwas carried out for Zn 2+ concentration aftercontact time of two and five hours. The resultsof the research indicated that free andimmobilized S. cerevisiae biomass couldabsorb Zn 2+ metal and decreased itsconcentration from 250-300 ppm to 20-50 ppm.The optimum contact time was reached at onehour, while optimum sorption process occurredat pH 5. At low concentration, less than20 ppm S. cerevisiae biomass absorbed lessZn 2+ The NaOH-treated biomass showed bettersorption capabilities compared to cells treatedby formaldehyde or heat treatments. Thecontinue experiment showed the high capacityof biomass treated with NaOH to absorb Zn 2+ ,until concentration 24,02- 47,95 ppm in thefirst sampling and 1,15-10,99 ppm in thesecond sampling. Combination adsorptionprocess using charcoal and zeolite couldadsorp remain concentration of Zn 2+ , so thatcould reached the limit concentration-allowed.RingkasanLogam berat di dalam air limbahmerupakan penyebab pencemaran lingkunganyang potensial. Pencemaran logam berat padaumumnya berasal dari industri penyepuhanlogam, tekstil, barang jadi lateks, serta industrilain. Pada proses industri barang jadi lateksdigunakan logam berat dalam bentuk ZnOsebagai akselerator proses vulkanisasi karet,sehingga ion Zn 2+ terbawa dalam air limbahindustri barang jadi dengan konsentrasimencapai 300 ppm, sedangkan ambang bataskonsentrasi yang diperbolehkan maksimaladalah 2,5 ppm. Cara kimia yang umum di-gunakan untuk menurunkan kandunganZn 2+ dalam air limbah adalah dengan caramenambahkan basa, umumnya NaOH atauCa(OH) 2 , sampai pH sekitar 11, sehinggalogam berat ini diendapkan sebagai hidroksida-nya. Namun demikian, cara ini sangat mahaldan beresiko munculnya pencemaran sekunderakibat kelebihan basa. Salah satu alternatifyang murah untuk penyerapan Zn 2+ adalahmenggunakan biosorben berupa biomassaSaccharomyces cerevisiae yang berasal darilimbah pabrik bioetanol.Penelitian dilakukan dengan mengguna-kan air limbah artifisial yang mengandung ionZn 2+ dengan konsentrasi 300 ppm. Limbahartifisial diatur pHnya antara 3-7. Biosorpsidilakukan dengan menambahkan biomassaS. cerevisiae bebas maupun yang diamobilisasidengan kertas saring. Pengamatan dilakukanterhadap kandungan Zn 2+ setelah waktu kontakdua dan lima jam. Hasil penelitian menunjuk-kan bahwa biomassa S. cerevisiae bebasmaupun amobil mampu menyerap logam Zn 2+dan menurunkan konsentrasinya dari 250-300 ppm menjadi 20-50 ppm. Waktu kontakoptimum dicapai setelah satu jam, sedangkanproses sorpsi optimum terjadi pada pH 5.Biomassa S. cerevisiae kurang efektifmenyerap logam Zn 2+ pada konsentrasi rendah,di bawah 20 ppm. Perlakuan biomassa meng-gunakan NaOH menunjukkan kemampuanpenyerapan yang lebih baik jika dibandingkandengan yang diperlakukan menggunakanformaldehida dan pemanasan. Percobaan padaaliran kontinyu yang menggunakan biomassayang diperlaukan menggunakan NaOH,menunjukkan bahwa limbah artifisial Zn 2+dapat diturunkan sampai konsentrasi 24,02-47,95 ppm pada sampling pertama, dan 1,15-10,99 ppm pada sampling kedua. Kombinasipenyerapan menggunakan arang aktif danzeolit dapat menyerap sisa Zn 2+ mencapai batasyang diperbolehkan.

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Uptake of lead, cadmium and zinc by a novel bacterial exopolysaccharide

Cited by 198 publications

References 25 publications

The role of extracellular exopolymers in the removal of phosphorus from activated sludge

The role of extracellular exopolymers in the removal of phosphorus from activated sludge

Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced byPseudoalteromonassp. Strain TG12

Biosorpsi logam Zn oleh biomassa Saccharomyces cerevisiae *) Biosorption of Zn metal by Saccharomyces cerevisiae biomass

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