Uptake of Vapour Phase [14C]Dodecane by Whole Mycelia of Cladosporium resinae

Abstract

2301The kinetics of uptake of n-alkanes by a filamentous fungus, Cladosporium resinae, were shown to be similar to hydrocarbon uptake kinetics described for yeasts. Transport of n-alkanes across the cell membrane was accompanied by the partitioning of substantial amounts of hydrocarbon on to the cell surface. Metabolic inhibitors significantly reduced uptake rates. Most [ 4C]dodecane was taken up by dodecane-grown mycelia; slower uptake was observed with mycelia grown on other n-alkanes or glucose. Uptake of dodecane was not restricted to specific regions of the mycelium, although higher concentrations of 4C-labelled compounds were observed at the hyphal tips.

Alkane utilisation by Cladosporium resinae: the importance of extended lag phases when assessing substrate optima

“…The removal of sub- strate always began when biomass started to increase, and never earlier, if corrections for evaporation were made. These findings accord with our previous report [12] that uptake of dodecane by C. resinae was significantly greater for mycelia pre-grown on that substrate than for mycelia pregrown on other alkanes. Relatively short alkanes within fuel have previously been reported as best able to support growth of C. resinae [6][7][8].…”

“…Each sample was vacuum-filtered through a preweighed glass fibre filter, washed on it twice with glass-distilled water and once with acetone, and dried to constant weight at 105°C. Such an acetone wash has previously been shown to remove only those hydrocarbons bound to the cell surface [12] and in this study did not affect the dry weight of cells grown on glucose. The protein concentration was measured [13] in washed culture samples solubilised in 1 M NaOH for 20 min at 85°C.…”

Factors affecting population development within seawater-displaced fuel tanks

“…The viable numbers of each species fell rapidly at the interface just before premature entry into stationary phase. The hydrophobic attraction of the cell surface for the hydrocarbon substrate is an essential feature of hydrocarbon uptake and metabolism [10][11][12] and is generally greater for hydrocarbon-utilisers than for non-utilisers [8,10]. However, the binding affinity varies considerably amongst hydrocarbon-utilising micro-organisms, and the order of succession observed here is in agreement with increasing affinity for the substrate ( Table 2).…”

Preliminary Characterizations of a Carbohydrate from the Concentrated Culture Filtrate from <i>Fusarium solani</i> and Its Role in Benzo[a]Pyrene Solubilization

“…This cytological observation underlines that this fungus has developed a strategy to enhance the bioavailability and gained access to hydrophobic compounds such as BaP. While numerous works concern the uptake of alkanes by fungi, and especially yeasts [9,10], the mechanisms of PAH solubilization and transport were rarely studied in fungi [6,11,12]. In order to better understand the interaction mechanisms between the fungal cells and PAH, we have undertaken the present investigation which reports the partial characterization of the concentrated fungal filtrate produced by the…”