Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

Duhazé, Claire; Gagneul, David; Leport, Laurent; Larher, F.; Bouchereau, Alain

doi:10.1016/j.plaphy.2003.06.002

Cited by 27 publications

(15 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is synthesized by the condensation of b-alanine and pantoic acid (Genschel et al 1999). In higher plants, three possible pathways of b-alanine biosynthesis have been reported; these are the spermidine route (Terano andSuzuki 1978, Rastogi andDavies 1989), the propionate route (Hatch andStumpf 1962, Rathinasabapathi 2002) and the uracil route (Walsh et al 2001, Duhazé et al 2003. Our results strongly suggest that the uracil route, at least, contributes to pantothenic acid synthesis in potato leaves.…”

Section: Biosynthesis Of Pantothenic Acid From Uracilsupporting

confidence: 50%

Dual function of pyrimidine metabolism in potato (Solanum tuberosum) plants: pyrimidine salvage and supply of β‐alanine to pantothenic acid synthesis

Katahira

Ashihara

2006

Physiologia Plantarum

View full text Add to dashboard Cite

Uridine and cytidine are major nucleosides and are produced as catabolites of pyrimidine nucleotides. To study the metabolic fates and role of these nucleosides in plants, we have performed pulse (2 h) and chase (12 h) experiments with [2-14 C]uridine and [2-14 C]cytidine and determined the activities of some related enzymes using tubers and fully expanded leaves from 10-week-old potato plants (Solanum tuberosum L.). In tubers, more than 94% of exogenously supplied [2-14 C]uridine and [2-14 C]cytidine was converted to pyrimidine nucleotides and RNA during 2-h pulse, and radioactivity in these salvage products still remained at 12 h after the chase. Little degradation of pyrimidine was found. A similar pyrimidine salvage was operative in leaves, although more than 20% of the radioactivity from [2-14 C]uridine and [2-14 C]cytidine was released as 14 CO 2 during the chase. Enzyme profile data show that uridine/cytidine kinase (EC 2.7.1.48) activity is higher in tubers than in leaves, but uridine nucleosidase (EC 3.2.2.3) activity was higher in leaves. In leaves, radioactivity from [U-14 C]uracil was incorporated into b-ureidopropionic acid, CO 2 , b-alanine, pantothenic acid and several common amino acids. Our results suggest two functions of uridine and cytidine metabolism in leaves; these nucleosides are not only substrates for the classical pyrimidine salvage pathways but also starting materials for the biosynthesis of b-alanine. Subsequently, some b-alanine units are utilized for the synthesis of pantothenic acid in potato leaves.

show abstract

Section: Biosynthesis Of Pantothenic Acid From Uracilsupporting

confidence: 50%

Dual function of pyrimidine metabolism in potato (Solanum tuberosum) plants: pyrimidine salvage and supply of β‐alanine to pantothenic acid synthesis

Katahira

Ashihara

2006

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Because radioactivity from [2‐ 14 C]pyrimidine precursors was not incorporated into β‐alanine, we were not able to follow the metabolic fate of β‐alanine, but this compound is a starting material for the synthesis of various compounds, including pantothenic acid and coenzyme A (Katahira and Ashihara 2005). In Limonium latifolium , which is a halotolerant, β‐alanine derived from uracil is utilised for the synthesis of β‐alanine betaine (Duhazé et al 2003). Stimulation of β‐UP degradation and β‐alanine synthesis by NaCl might therefore be related to the synthesis of compatible solutes in the mangrove cells.…”

Section: Resultsmentioning

confidence: 99%

Effect of short‐term salt stress on the metabolic profiles of pyrimidine, purine and pyridine nucleotides in cultured cells of the mangrove tree, Bruguiera sexangula

Suzuki-Yamamoto

Mimura

Ashihara

2006

Physiologia Plantarum

View full text Add to dashboard Cite

Effect of short-term salt stress on the metabolic profiles of pyrimidine, purine and pyridine nucleotides in cultured cells of the mangrove tree, Bruguiera sexangula To investigate the short-term (3 h) effect of salt on the metabolism of purine, pyrimidine and pyridine nucleotides in mangrove (Bruguiera sexangula) cells, we examined the uptake and overall metabolism of radiolabelled intermediates involved in the de novo pathways and substrates of salvage pathways for nucleotide biosynthesis in the presence and absence of 100 mM NaCl. Uptake by the cells of substrates for the salvage pathways was much faster than uptake of intermediates of the de novo pathways. The activity of the de novo pyrimidine biosynthesis estimated by [2-14 C]orotate metabolism was not significantly affected by the salt. About 20-30% of [2-14 C]uridine, [2-14 C]uracil and more than 50% of [2-14 C]cytidine were salvaged for pyrimidine nucleotide biosynthesis. However, substantial quantities of these compounds were degraded to 14 CO 2 via b-ureidopropionate (b-UP), and degradation of b-UP was increased by the salt. The activities of the de novo pathway, estimated by [2-14 C] 5-aminoimidazole-4-carboxamide ribonucleoside, and the salvage pathways from [8-14 C]adenosine and [8-14 C]guanosine for the purine nucleotide biosynthesis were not influenced by the salt. Most [8-14 C]hypoxanthine was catabolised to 14 CO 2 , and other purine compounds are also catabolised via xanthine. Purine catabolism was stimulated by the salt.[ 3 H]Quinolinate, [carbonyl-14 C]nicotinamide and [carboxyl-14 C]nicotinic acid were utilised for the biosynthesis of pyridine nucleotides. The salvage pathways for pyridine nucleotides were significantly stimulated by the salt. Trigonelline was synthesised from all pyridine precursors that were examined; its synthesis was also stimulated by the salt. We discuss the physiological role of the salt-stimulated reactions of nucleotide metabolism.

show abstract

“…β-Alanine is a non-protein amino acid synthesized mainly by polyamine (i.e. spermine and spermidine) degradation and involved in coenzyme A (CoA) biosynthesis via pantothenate [ 44 , 45 ], uracil [ 46 ] or possibly from propionate [ 47 ]. In opium poppy cells, β-alanine was only detected in elicitor-treated cultures suggesting a role in the defence response.…”

Section: Discussionmentioning

confidence: 99%

Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures

et al. 2008

View full text Add to dashboard Cite

Background: Opium poppy (Papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids and has emerged as a model system to study plant alkaloid metabolism. The plant is cultivated as the only commercial source of the narcotic analgesics morphine and codeine, but also produces many other alkaloids including the antimicrobial agent sanguinarine. Modulations in plant secondary metabolism as a result of environmental perturbations are often associated with the altered regulation of other metabolic pathways. As a key component of our functional genomics platform for opium poppy we have used proton nuclear magnetic resonance ( 1 H NMR) metabolomics to investigate the interplay between primary and secondary metabolism in cultured opium poppy cells treated with a fungal elicitor.

show abstract

Uracil as one of the multiple sources of β-alanine in Limonium latifolium, a halotolerant β-alanine betaine accumulating Plumbaginaceae

Cited by 27 publications

References 30 publications

Dual function of pyrimidine metabolism in potato (Solanum tuberosum) plants: pyrimidine salvage and supply of β‐alanine to pantothenic acid synthesis

Dual function of pyrimidine metabolism in potato (Solanum tuberosum) plants: pyrimidine salvage and supply of β‐alanine to pantothenic acid synthesis

Effect of short‐term salt stress on the metabolic profiles of pyrimidine, purine and pyridine nucleotides in cultured cells of the mangrove tree, Bruguiera sexangula

Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures

Contact Info

Product

Resources

About