Uranium (238U)-induced ROS and cell cycle perturbations, antioxidant responses and erythrocyte nuclear abnormalities in the freshwater iridescent shark fish Pangasius sutchi

Annamalai, Sathesh Kumar; Arunachalam, Kantha Deivi

doi:10.1016/j.aquatox.2017.03.002

Cited by 29 publications

(6 citation statements)

References 62 publications

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“…Cells exposed to uranium could generate many reactive oxygen species (ROS), which eventually leads to cell inactivation and even necrosis. The considerably increased ROS may be to scavenge the overproduction of superoxide anions under oxidative stress induced by 238 U accumulation . Therefore, an assay was designed to measure the ROS levels of the cells treated with or without COS-HOPO.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The considerably increased ROS may be to scavenge the overproduction of superoxide anions under oxidative stress induced by 238 U accumulation. 54 Therefore, an assay was designed to measure the ROS levels of the cells treated with or without COS-HOPO. As shown in Figure 2C, the level of intracellular ROS in the group treated with UO 2 2+ dramatically increased 2-fold compared with the mock group, whereas the U(VI)-induced ROS production decreased by 35.8% in the group cotreated with 50 μg/mL COS-HOPO and 49.6 μM uranium U(VI), and 23.7% in the group cotreated with ZnNa 3 -DTPA and 49.6 μM uranium U(VI) compared with the group treated with solely UO 2 2+ .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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3,2-Hydroxypyridinone-Grafted Chitosan Oligosaccharide Nanoparticles as Efficient Decorporation Agents for Simultaneous Removal of Uranium and Radiation-Induced Reactive Oxygen Species in Vivo

et al. 2018

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Most of the key radionuclides in the nuclear fuel cycle, such as actinides, possess a combination of heavy metal chemotoxicity and radiotoxicity and therefore represent a severe threat to the ecological environment and public safety. The radiotoxicity originates from direct radiation-induced organ damage and indirect damage, mostly through radiation-induced reactive oxygen species (ROS). Although effective chelating agents that can accelerate the excretion of actinides, such as uranium, have been developed in the past several decades, very few of them can reduce radiation-induced damage from internal contamination. In fact, the strategy of simultaneous removal of actinides and their induced-ROS in vivo has scarcely been considered. Here, we report a 3,2-hydroxypyridinone-grafted chitosan oligosaccharide nanoparticle (COS-HOPO) as a new type of decorporation agent that is effective for the removal of both uranium and ROS in vivo. The cytotoxicity and decorporation assays indicate that the marriage of chitosan oligosaccharide (COS) and hydroxypyridinone (HOPO) gives rise to a remarkable decrease in toxicity and promotion of the uranium removal capability from both kidneys and femurs. The decorporation efficacy can reach up to 43% in rat proximal tubular epithelial cells (NRK-52E), 44% in kidneys, and 32% in femurs. Moreover, the ROS levels of the cells treated with COS-HOPO are significantly lower than those of the control group, implying a promising radiation protection effect. The detoxification mechanism of COS-HOPO is closely related to both chelating U(VI)-and scavenging U(VI)-induced intracellular ROS.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

3,2-Hydroxypyridinone-Grafted Chitosan Oligosaccharide Nanoparticles as Efficient Decorporation Agents for Simultaneous Removal of Uranium and Radiation-Induced Reactive Oxygen Species in Vivo

et al. 2018

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“…The internal contamination of uranium in cells results in the increased activities of lipid peroxidase (LPO) and superoxide dismutase (SOD) and the decreased activity of catalase (CAT), indicating the generation of excessive amounts of superoxide radicals (˙O 2 − ) as reported. 10 The reduction of superoxide radicals to H 2 O 2 was further enhanced by the catalysis of SOD. 10 H 2 O 2 is well-known as a member of the ROS family, and supraphysiological concentrations of H 2 O 2 (>100 nM) will lead to damage to biomolecules due to the generation of hydroxyl radicals (˙OH), 27 the strongest ROS species in vivo , via the Fenton reaction of H 2 O 2 and Fe 2+ /Fe 3+ .…”

Section: Resultsmentioning

confidence: 99%

“…And the case is even worse when exposed to highly radioactive uranium isotopes such as U-233 and U-230. 10 Hence, the efficient and targeted removal of uranium and excess ROS in the accumulation sites is the most crucial in treating uranium-contaminated individuals.…”

Section: Introductionmentioning

confidence: 99%

Decorporation of uranyl in kidneys using an engineered nanocomposite

Shi

Wang

Sun

et al. 2022

Environ. Sci.: Nano

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“…Instead, our model concluded that there was a significant offset in cell number and PDs over the entire growth curve. It is hypothesized that the delay in mitosis may be attributed to U being able to keep cells locked in S and G2 phases of the cell cycle, as demonstrated in at least one in vivo model [ 47 ]. An alternative hypothesis is that the delay in proliferation is once again a product of elevated necrosis or apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Environmentally Relevant Levels of Depleted Uranium Impacts Dermal Fibroblast Proliferation, Viability, Metabolic Activity, and Scratch Closure

Cruz

Buscaglia

Salanga

et al. 2021

Toxics

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Uranium (U) is a heavy metal used in military and industrial settings, with a large portion being mined from the Southwest region of the United States. Uranium has uses in energy and military weaponry, but the mining process has released U into soil and surface waters that may pose threats to human and environmental health. The majority of literature regarding U’s human health concern focuses on outcomes based on unintentional ingestion or inhalation, and limited data are available about its influence via cutaneous contact. Utilizing skin dermis cells, we evaluated U’s topical chemotoxicity. Employing soluble depleted uranium (DU) in the form of uranyl nitrate (UN), we hypothesized that in vitro exposure of UN will have cytotoxic effects on primary dermal fibroblasts by affecting cell viability and metabolic activity and, further, may delay wound healing aspects via altering cell proliferation and migration. Using environmentally relevant levels of U found in water (0.1 μM to 100 μM [UN]; 23.8–23,800 ppb [U]), we quantified cellular mitosis and migration through growth curves and in vitro scratch assays. Cells were exposed from 24 h to 144 h for a time-course evaluation of UN chemical toxicity. The effects of UN were observed at concentrations above and below the Environmental Protection Agency threshold for safe exposure limits. UN exposure resulted in a dose-dependent decrease in the viable cell count; however, it produced an increase in metabolism when corrected for the viable cells present. Furthermore, cellular proliferation, population doubling, and percent closure was hindered at levels ≥10 μM UN. Therefore, inadvertent exposure may exacerbate pre-existing skin diseases in at-risk demographics, and additionally, it may substantially interfere in cutaneous tissue repair processes.

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Uranium (238U)-induced ROS and cell cycle perturbations, antioxidant responses and erythrocyte nuclear abnormalities in the freshwater iridescent shark fish Pangasius sutchi

Cited by 29 publications

References 62 publications

3,2-Hydroxypyridinone-Grafted Chitosan Oligosaccharide Nanoparticles as Efficient Decorporation Agents for Simultaneous Removal of Uranium and Radiation-Induced Reactive Oxygen Species in Vivo

3,2-Hydroxypyridinone-Grafted Chitosan Oligosaccharide Nanoparticles as Efficient Decorporation Agents for Simultaneous Removal of Uranium and Radiation-Induced Reactive Oxygen Species in Vivo

Decorporation of uranyl in kidneys using an engineered nanocomposite

Environmentally Relevant Levels of Depleted Uranium Impacts Dermal Fibroblast Proliferation, Viability, Metabolic Activity, and Scratch Closure

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