Urea Breath Tests for the Detection of <i>Helicobacter pylori</i> Infection

Bazzoli, Franco; Zagari, M.; Fossi, S.; Pozzato, P.; Ricciardiello, Luigi; Mwangemi, C.; Roda, Aldo; Roda, Enrico

doi:10.1111/j.1523-5378.1997.06b10.x

Cited by 49 publications

(38 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some centres use a 14 C-based test, which is cheaper but has the significant disadvantage of radiation for the patient. 42 Similar to biopsy-based tests, false-negative urea breath tests can occur in patients on treatment with proton pump inhibitors or antibiotics. H. pylori infection elicits a vigorous host immune response, and the presence of H. pylori antibodies in the blood can be used to diagnose the presence of the infection.…”

Section: Noninvasive Testsmentioning

confidence: 99%

The burden of Helicobacter pylori infection in Europe

O'Connor¹,

Sebastian²

2003

Aliment Pharmacol Ther

View full text Add to dashboard Cite

Section: Noninvasive Testsmentioning

confidence: 99%

The burden of Helicobacter pylori infection in Europe

O'Connor¹,

Sebastian²

2003

Aliment Pharmacol Ther

View full text Add to dashboard Cite

“…The presence of H. pylori in dental plaque of patients both with and without stomach disorders has been investigated by bacterial culture and PCR methods. Most studies have failed to isolate H. pylori by culture from dental plaque of subjects with gastric infection (4,5,6). PCR analysis of dental plaque from subjects with gastric infection has yielded more conflicting data, with H. pylori DNA found frequently in some studies (4, 35) but absent or found at extremely low frequencies in other studies (5,24).…”

Section: Discussionmentioning

confidence: 99%

“…Most studies have failed to isolate H. pylori by culture from dental plaque of subjects with gastric infection (4,5,6). PCR analysis of dental plaque from subjects with gastric infection has yielded more conflicting data, with H. pylori DNA found frequently in some studies (4, 35) but absent or found at extremely low frequencies in other studies (5,24). In the dental plaque of healthy subjects, culture methods have detected H. pylori at a high frequency in a single study (32), while PCR has only very rarely detected H. pylori at high frequency (4).…”

Section: Discussionmentioning

confidence: 99%

“…Since invasive methods are expensive, less-invasive methods such as serologic examination of blood and the urea breath test are becoming more popular (47). However, positive results by blood serology do not necessarily allow the delineation of a current active H. pylori infection from a past H. pylori infection, while urea breath tests require expensive, specialized equipment and reagents (5).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque

Goosen¹,

Theron²,

Ntsala³

et al. 2002

J Clin Microbiol

View full text Add to dashboard Cite

A novel heminested PCR protocol was developed for the specific detection of Helicobacter pylori at low copy numbers. A set of primers specific for the phosphoglucosamine mutase gene (glmM) of H. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a 496-bp fragment. By using agarose gel electrophoresis for detection of the heminested PCR-amplified products, amplification of H. pylori genomic DNA was achieved at concentrations as low as 0.1 pg, equivalent to 5 ؋ 10 2 bacteria. A study was subsequently undertaken to evaluate the heminested PCR for detection of H. pylori in dental plaque and saliva. Specimens collected from 58 individuals were cultured, and PCR was subsequently performed on the oral cultures. Identification of H. pylori in the same series of saliva and dental plaque specimens was carried out with PCR using a primer pair specific for the H. pylori urease B gene and by the heminested PCR assay. The identity of the amplified products was confirmed by DNA sequencing. Our results demonstrate that the heminested PCR assay was specific for detection of H. pylori, yielding no false-positive results, and that H. pylori had a low prevalence (approximately 3%) in specimens obtained from the oral cavity.

show abstract

“…Breath testing, which uses either a radioactive isotope ( 14 C-labelled urea) or stable, nonradioactive isotope ( 13 C) is an exquisitely sensitive and specific noninvasive test both in adults (32) and children (33,34). In addition, the accuracy of the test has been proposed to correlate with bacterial load in children (34,35).…”

Section: Diagnostic Tests For H Pylori Infectionmentioning

confidence: 99%

Current Therapy forHelicobacter pyloriInfection in Children and Adolescents

Gold

1999

Canadian Journal of Gastroenterology

View full text Add to dashboard Cite

Helicobacter pyloriinfects approximately 50% of the world’s population and is a definitive cause of gastroduodenal disease (ie, gastritis, duodenal and gastric ulcers) in children and adults. Four consensus conferences held around the globe have brought together clinicians, scientists, epidemiologists and health care economists to discuss the role of the gastric pathogenH pyloriin human gastroduodenal disease. At each of these conferences, the overriding objective was to reach a consensus on the development of practical guidelines for the diagnosis and treatment ofH pylori-infected individuals. However, it was not until the CanadianH pyloriConsensus Conference, held in November 1997, that the issues ofH pyloriinfection in children were addressed. Therapies forH pyloriinfection in children, presented in part at the First Canadian PaediatricH pyloriConsensus Conference, held in Victoria, British Columbia, November 1998, are reviewed in this paper.

show abstract

Urea Breath Tests for the Detection of Helicobacter pylori Infection

Cited by 49 publications

References 14 publications

The burden of Helicobacter pylori infection in Europe

The burden of Helicobacter pylori infection in Europe

Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque

Current Therapy forHelicobacter pyloriInfection in Children and Adolescents

Contact Info

Product

Resources

About