The objective of this study was to evaluate the preservative and upgrading potential of urea added to whole-crop triticale. Triticale, harvested at different growth stages according to Feekes' scale (10.51, 10.54, 11.1 and 11.2), was ensiled with four levels of urea (0, 30, 60 and 90 g kg −1 dry matter (DM)) and stored in plastic bags for 60 days. Data were analysed as a factorial design with growth stage and urea level as the main factors. Urea was extensively hydrolysed (more than 90% of added urea). Urea breakdown was not affected by urea level (P > 0.05) but decreased significantly with growth stage (P < 0.001). Microbial activity measured by pH, volatile fatty acid production and total non-structural carbohydrate concentration was significantly reduced by the urea level. Urea treatment significantly (P < 0.001) increased water-soluble and ammonium nitrogen; more than 85% of the added nitrogen was retained. Application of urea at a rate of 60 and 90 g kg −1 DM significantly (P < 0.05) reduced the neutral detergent fibre (NDF) content and increased (P < 0.05) the NDF and acid detergent fibre in vitro digestibility. Whole-crop triticale harvested at later growth stages (approximately 60% DM) can be effectively preserved and upgraded by ensiling with 60-90 g urea kg −1 DM.