Urease and Nitrification Retardation Properties in Natural Essential Oils and Their By‐products

Patra, D. D.; Kiran, Usha; Pande, Preeti

doi:10.1080/00103620600710306

Cited by 37 publications

(20 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PLFA profiles showed that the general microbial community composition was changed by the addition of dicyandiamide, as also found by Patra et al (2006). The PLFAs observed in the first principal component are associated with Gram negative species which would also include the nitrifying bacteria, although they represent only a very minor component of this community.…”

Section: Resultsmentioning

confidence: 85%

Effect of the nitrification inhibitor dicyandiamide on microbial communities and N2O from an arable soil fertilized with ammonium sulphate

Carneiro

Cardenas²,

Hatch³

et al. 2009

Environ Chem Lett

View full text Add to dashboard Cite

Nitrous oxide (N 2 O) affects climate change as a greenhouse gas and indirectly contributes to stratospheric ozone depletion. The main source of N 2 O in soils is denitrification which requires high soil moisture, carbon and nitrate. Nitrification inhibitors can be used to mitigate emissions of N 2 O from soils. In Portugal, fertilisers are often applied when soils are still relatively warm and moist conditions conducive to denitrification. A Portuguese arable soil was inhibited with dicyandiamide, a nitrification inhibitor and the effect on soil microbiological activity and composition was determined after 46 days. Soils were then incubated and received carbon and ammonium under high soil water conditions and mineral N and N 2 O fluxes were measured during 22 days. We found that dicyandiamide decreased microbial populations and activity, but did not alter composition. Pre-conditioning of the soil with dicyandiamide was 80% more effective in reducing fluxes of N 2 O than simultaneous application with fertiliser.

show abstract

Section: Resultsmentioning

confidence: 85%

Effect of the nitrification inhibitor dicyandiamide on microbial communities and N2O from an arable soil fertilized with ammonium sulphate

Carneiro

Cardenas²,

Hatch³

et al. 2009

Environ Chem Lett

View full text Add to dashboard Cite

show abstract

“…Neem (Azadirachta indica) products (Joseph and Prasad 1993;Kumar et al 2007) and plant oils (Erickson et al 2000;Patra et al 2006) have been shown to be as effective as synthetic inhibitors. However, difficulties with consistent delivery of the active ingredient in quantities that ensure the inhibitor is present in soils for long enough to provide sufficient control of nitrification are likely to restrict wide use.…”

Section: Production Of Nitrification Inhibitors By Plant?mentioning

confidence: 99%

Plant-based manipulation of nitrification in soil: a new approach to managing N loss?

Fillery

2007

Plant Soil

View full text Add to dashboard Cite

Much work has gone into the management of nitrification through applications of chemicals known to inhibit enzyme function in nitrifiers with indifferent outcomes when tested in the field. Much less attention has been focused on the capacity of plants to modify nitrification in situ. Subbarao and coworkers in a series of neat and elegant studies have confirmed that a tropical grass species, Brachiaria humidicola, produces chemicals that inhibit nitrification in soil. Critical to the work was the use of a Nitrosomonas europaea strain (nitrifying bacteria) that had been specifically constructed to produce bioluminescence due to the expression of ''luxAB' genes during nitrification. This application led to the development of an assay that enabled the suppression of nitrification to be assessed directly. They produce evidence that the production of chemicals by Brachiaria humidicola roots, described as biological nitrification inhibitors (BNIs), is under plant control. However, the triggers or molecular controls for BNI production have yet to be ascertained. Examination of the capacity of major crops to produce BNIs, including wheat (Triticum aestivum), barley (Hordeum vulgare), rice (Oryza sativa) and maize (Zea mays) indicate that these do not have this capacity. Work is needed on wild relatives of these crops and the major temperate grass species such as Lolium perenne to determine whether these have the capacity to produce BNIs with an aim to introduce this capacity into domesticated lines. The work of Subbarao et al. highlights how molecular biology can be used to introduce traits into micro-organisms responsible for key soil N transformations in a way that facilitates analysis of the interaction between plants and the soil environment so crucial to their growth and survival.

show abstract

“…For example, neem (Azadirachta indica A. Juss), karanja (Pongamia glabra Vent)-products (Parmar et al 1976; and certain plant oils (Patra et al 2006) have been reported to show inhibitory effect on soil nitrification. In most cases, the active chemical constituents were either undefined or there were difficulties with consistent delivery of active ingredients in sufficient quantities to maintain inhibition; consequently they have had only limited value in production agriculture (Fillery 2007).…”

Section: Introductionmentioning

confidence: 98%

Free fatty acids from the pasture grass Brachiaria humidicola and one of their methyl esters as inhibitors of nitrification

et al. 2008

View full text Add to dashboard Cite

The tropical pasture grass, Brachiaria humidicola (Rendle) Schweick, produces nitrification inhibitory compounds (termed biological nitrification inhibitors or BNIs) in its shoot and root tissues and releases BNIs from its roots. In the present study, two BNI compounds were isolated and identified from the shoot tissue of B. humidicola using activity-guided fractionation. The recombinant Nitrosomonas europaea containing luxAB genes derived from the bioluminescent marine gram-negative bacterium Vibrio harveyi, were used to determine BNI activity. The BNI compounds in the shoot tissue were identified as linoleic acid (LA) and linolenic acid (LN) using authenticchemicals obtained from ©Sigma (ED 80 16.0 μg ml −1 for both LA and LN) for verification. None of the other tested free fatty acids namely stearic acid, oleic acid, arachidonic acid, and cis-vaccenic acid showed any inhibitory effect on nitrification. Among the fatty acid methyl esters (FAME) evaluated [methyl oleate, methyl linoleate (LA-ME) and methyl linoleneate (LN-ME)], only LA-ME showed an inhibitory effect (ED 80 8.0 μg ml −1 ). The inhibitory effect of LA, LN and LA-ME in the soil was stable for 120 days at 20°C. Soil treated with LA, LN and LA-ME showed a very low accumulation of NO 3 − and the maintenance of soil inorganic N in the NH 4 + form. The inhibitory effect of LA-ME on soil nitrification was greater than that of LA or LN. In addition to BNI activity, both LA and LA-ME showed a suppressive effect on urea hydrolysis in soil. Both LA and LN blocked the AMO (ammonia monooxygenase) and HAO (hydroxylamino oxidoreductase) enzymatic pathways in Nitrosomonas. Since LA and LN can be produced from vegetable oils such as soybean, flax or sunflower, they have the potential for use as nitrification inhibitors in production agriculture.

show abstract

Urease and Nitrification Retardation Properties in Natural Essential Oils and Their By‐products

Cited by 37 publications

References 18 publications

Effect of the nitrification inhibitor dicyandiamide on microbial communities and N2O from an arable soil fertilized with ammonium sulphate

Effect of the nitrification inhibitor dicyandiamide on microbial communities and N2O from an arable soil fertilized with ammonium sulphate

Plant-based manipulation of nitrification in soil: a new approach to managing N loss?

Free fatty acids from the pasture grass Brachiaria humidicola and one of their methyl esters as inhibitors of nitrification

Contact Info

Product

Resources

About