Uric acid detection in visible spectrum

Yaacob, Afiqah; Ngajikin, Nor Hafizah; Rashid, Nurfatihah Che Abd; Ali, Siti Hajar Aminah; Yaacob, Maslina; Isaak, Suhaila; Cholan, Noran Azizan

doi:10.12928/telkomnika.v18i4.14993

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…The fluorescence intensity after the addition of uric acid to the complex, Alk-NGQDs•Crn is 425 while for other interferons are 484, 482, 478, 461, 492, 489, 488, 496, and 460 for Ca 2+ , urea, glucose, ascorbic acid, Na + , Zn 2+ , K + , Mg 2+ , and Fe 3+ respectively. The interaction of the sodium in the Alk-NGQDs with the uric acid produces monosodium urate and causes a slight decrease in fluorescence intensity [85]. In addition to that, uric acid is known to be a fluorescence quencher [86].…”

Section: Effect Of Time and Interferonsmentioning

confidence: 99%

Alkaline N-GQDs fluorescent probe for the ultrasensitive detection of creatinine

Ravi¹,

Subramaniyam²,

Saravanakumar³

et al. 2022

Methods Appl. Fluoresc.

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Creatinine (Crn) is an important excretory product of the human body. Medical laboratory technology has improved over years and brought many advancements in clinical diagnostics equipment, and testing techniques and made the tests more efficient. Yet, the quantitative analysis of Crn is still carried out by the classical Jaffe’s reaction (using Picric acid (PA) with NaOH) method. Since PA is hazardous to human health, alternative solutions such as; nanoparticles and surface-modified nanoparticles can be used. Exploring the optoelectronic properties of carbon-based quantum dots for biomolecule sensing is of current interest among researchers. Nitrogen functionalized graphene quantum dots (Alk-NGQDs) measured featured Crn easier and reduced the time taken for the test carried out in laboratories. The synthesized Alk-NGQDs optical, structural, morphological properties, surface and compositions are studied through XPS, HRTEM, XRD, FTIR, and spectroscopic techniques. Alk-NGQDs at alkaline conditions (pH 9.5) form a stable complex with Crn through intermolecular charge transfer (ICT). The fluorescence titration method is used to sense Crn in commercial Crn samples and human blood serum. To understand the efficacy of sensing creatinine using Alk-NGQDs, working concentration, fluorescence quantum yield, the limit of detection, and quenching constant are calculated using the Stern-Volmer plot. The emission property of Alk-NGQDs is aimed to bring an alternative to the traditional colorimetric Jaffe’s reaction.

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Section: Effect Of Time and Interferonsmentioning

confidence: 99%

Alkaline N-GQDs fluorescent probe for the ultrasensitive detection of creatinine

Ravi¹,

Subramaniyam²,

Saravanakumar³

et al. 2022

Methods Appl. Fluoresc.

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“…A non-invasive alternative of blood glucose monitoring is introduced in [6], based on the detection of the optical density of the solution samples by means of a spectrophotometer, and then converting it into the corresponding glucose concentration by using the Beer–Lambert Law, with the help of a concentration curve. In [7] Ocean Optics Ocean View spectrometer operating software is used to obtain and process data from spectrometer, and get the transmittance (then, the absorbance) of a uric acid solution, from which to calculate uric acid concentration by using a concentration curve. The authors of [8] present and validate a quick and sensitive spectrophotometric method for quantitative determination of gliquidone in bulk drug, pharmaceutical formulations and human serum, based on the absorbance readings and their transformation into concentration through a calibration curve of the absorbance over the concentration.…”

Section: Introductionmentioning

confidence: 99%

Misuse of Beer–Lambert Law and other calibration curves

Delgado

2022

R. Soc. open sci.

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Calibration curves allow instrument calibration by predicting the concentration of an analyte in a sample from the reading of the instrument. This curve is constructed as the regression straight line that best fits the relationship between some known concentration standards and their respective instrument readings. An example is the Beer–Lambert Law, used to predict the concentration of a new sample from its absorbance obtained by spectrometry. The issue is that usually this methodology is misapplied. In this paper, we want to clarify this point, explaining what the error consists of and how (easily) to fix it, with the intention of ensuring that it does not continue to be reproduced in the experimental scientific work.

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“…The basic operating principle of the fiber optic sensor is that the light signal travelling through an optical fiber changes when it is subjected to chemical or physical stimulus [13]. Such mechanism allows the fiber optic sensor to find applications in detecting properties such as temperature [14], [15], liquid level [16], [17], refractive index [18]- [20], uric acid concentration [21], [22] and ammonia concentration [2], [6]- [11]. For ammonia detection in water, many methods have engineered the fiber such that a part of the cladding of the fiber sensor is removed through chemical etching, tapering or a combination of etching and tapering.…”

Section: Introductionmentioning

confidence: 99%

Optimization of light source wavelength for ammonia detection in water

Rashid

Cholan²,

Tay³

et al. 2022

TELKOMNIKA

Self Cite

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Optimization of light source wavelength for ammonia detection in surface water is presented in this work. For the ammonia detection, the surface water sample is mixed with sodium chloride and nessler reagent, whereas the sensor head consists of unclad plastic optical fiber. The unclad region has a length of 1 cm and the cladding is removed by immersing it in acetone solution. Experimental results indicate that the output light intensity of the sensor has linear relationship with the ammonia concentration. At the wavelength of 510 nm, the output light increases linearly as the ammonia concentration varies from 0.07 mg/L to 8.97 mg/L. At the same wavelength, the proposed sensor achieves the sensitivity of 0.0139 (mg/L) -1 , accuracy of 99.59% and resolution of 0.72 µg/L. The analysis of light source wavelength reveals that a wavelength range from 450 nm to 580 nm produces the optimized performances. Within this wavelength range, the proposed sensor achieves sensitivity of higher than 0.01 (mg/L) -1 , accuracy of higher than 99% and resolution of less than 1 µg/L.

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Uric acid detection in visible spectrum

Cited by 4 publications

References 19 publications

Alkaline N-GQDs fluorescent probe for the ultrasensitive detection of creatinine

Alkaline N-GQDs fluorescent probe for the ultrasensitive detection of creatinine

Misuse of Beer–Lambert Law and other calibration curves

Optimization of light source wavelength for ammonia detection in water

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