Urinary 3-methylhistidine excretion increases with repeated weight training exercise

Pivarnik, James M.; Hickson, James F.; Wolinsky, Ira

doi:10.1249/00005768-198906000-00009

Cited by 33 publications

(12 citation statements)

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“…The results of the current study demonstrate that the myofibrillar proteins titin and nebulin are decreased, via either direct damage or degradation, following eccentric resistance exercise in humans. These data are supported by the findings that mixed skeletal muscle protein degradation (i.e., when all of the muscle proteins are considered together) and 3‐methylhistidine excretion, a component of actin and myosin that reflects breakdown of these two myofibrillar proteins, are elevated following resistance exercise 14, 15. However, it is also possible that, following the exercise bout, the synthesis rates of titin and nebulin, relative to the synthesis rates of other muscle proteins or relative to the change in degradation rates of titin and nebulin, were decreased.…”

Section: Discussionmentioning

confidence: 59%

Titin and nebulin content in human skeletal muscle following eccentric resistance exercise

et al. 2002

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We measured titin and nebulin content in muscle biopsies from the vastus lateralis before and 24 h after one bout of high-intensity eccentric knee extensor resistance exercise in seven men (26 +/- 3 years). Titin and nebulin content were significantly (P < 0.05) reduced after exercise by 30 and 15%, respectively. These results suggest that the structural components of the myofibrillar apparatus are degraded following high-intensity eccentric resistance exercise in humans. Loss of these proteins may have important implications for the mechanisms regulating the adaptive response of skeletal muscle to resistance exercise.

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Section: Discussionmentioning

confidence: 59%

Titin and nebulin content in human skeletal muscle following eccentric resistance exercise

et al. 2002

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“…3-Methylhistidine is an amino acid located primarily in skeletal muscle from the post-translation modiWcation of speciWc histidine residues in myoWbrillar proteins (Lukaski et al 1981). During muscle protein catabolism, the released 3-methylhistidine is neither re-utilized for protein synthesis nor metabolized oxidatively but, instead, is quantitatively excreted in the urine (Lukaski et al 1981); therefore, it serves as a useful indicator of myoWbrillar protein degradation (Pivarnik et al 1989). Our results of no signiWcant eVect from protein supplementation on urinary 3-methylhistidine excretion is in agreement with Campbell et al (1995) who also found no eVect from protein supplementation (0.8-1.6 g/kg/day) during 12 weeks of RT on urinary 3-methylhistidine levels in older adults.…”

Section: Discussionmentioning

confidence: 99%

Protein supplementation before and after resistance training in older men

et al. 2006

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We determined the effects of protein supplementation immediately before (PRO-B) and after (PRO-A) resistance training (RT; 12 weeks) in older men (59-76 years), and whether this reduces deficits in muscle mass and strength compared to younger men (18-40 years). Older men were randomized to PRO-B (0.3 g/kg protein before RT + placebo after RT, n=9), PRO-A (placebo before + protein after RT, n=10), or PLA (placebo before and after RT, n=10). Lean tissue mass, muscle thickness of the elbow, knee, and ankle flexors and extensors, and leg and bench press strength were measured before and after RT and compared to databases of younger subjects (n=22-60). Myofibrillar protein degradation (3-methylhistidine) and bone resorption (cross-linked N-telopeptides) were also measured before and after RT. Lean tissue mass, muscle thickness (except ankle dorsi flexors), and strength increased with training (P<0.05), with little difference between groups. There were no changes in 3-methylhistidine or cross-linked N-telopeptides. Before RT, all measures were lower in the older compared to younger groups (P<0.05), except for elbow extensor muscle thickness. Following training, muscle thickness of the elbow flexors and ankle dorsi flexors and leg press strength were no longer different than the young, and elbow extensor muscle thickness was greater in the old men (P<0.05). Supplementation with protein before or after training has no effect on muscle mass and strength in older men. RT was sufficient to overcome deficits in muscle size of the elbow flexors and ankle dorsi flexors and leg press strength in older compared to younger men.

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“…On the other hand, an increased myo®bril turnover rate might be essential for allowing a rapid rearrangement and recovery of skeletal muscle tissue between frequent bouts of exercise. Both reduced (Villani 1987) and increased (Pivarnik et al 1989) 3-MeH excretion have been reported as a result of strength training. Increased creatinine excretion after heavy training supports an increased rate of skeletal muscle nitrogen turnover, but the fact that the increase in creatine excretion was only half of the increase in 3-MeH excretion indicates that dierent pools of skeletal muscle nitrogen are possibly aected in dierent ways.…”

Section: Hormone Statusmentioning

confidence: 98%

Changes in human skeletal muscle contractility and hormone status during 2 weeks of heavy strength training

Raastad¹,

Glomsheller²,

Bjøro³

et al. 2001

European Journal of Applied Physiology

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To examine neuromuscular and hormone changes during 2 weeks of heavy strength training, 18 weight-trained male students were recruited either into a heavy training group (HT, n = 11) or into a control group (Ctr, n = 7). The heavy training protocol consisted of leg-extensor workouts performed daily, while workouts were performed twice a week in the Ctr group. A test of one repetition maximum (1 RM) was performed before heavy training and on the 2nd day after heavy training. Isokinetic knee extensions, electrical stimulation, and squat jumps were performed before, on the 8th day of heavy training, and on the 4th day after heavy training. Morning blood samples (0800 hours) were drawn before, on the 8th day of heavy training, and on the 4th day after heavy training. Before, and on the 5th day after heavy training, 24 h urine samples were collected. The 1 RM leg press increased by 6 (SEM 2)% in the HT group. Testosterone and insulin-like growth factor-1 concentrations were respectively 12 (SEM 5)% and 11 (SEM 3)% lower than baseline on the 8th day of heavy training; however, hormone levels were back to baseline on the 4th day after heavy training. A significant correlation between individual changes in 1 RM leg press and changes in testosterone concentrations was observed in the HT group (r = 0.69). In the HT group, 24 h urinary catecholamine excretion increased by 26 (SEM 12)%, 3-methylhistidine excretion increased by 21 (SEM 6)% and creatinine excretion increased by 11 (SEM 5)%. There were no significant changes in the Ctr group. This work addresses the role of changes in basal hormone status (morning samples) for skeletal muscle adaptation to heavy strength training.

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Urinary 3-methylhistidine excretion increases with repeated weight training exercise

Cited by 33 publications

References 0 publications

Titin and nebulin content in human skeletal muscle following eccentric resistance exercise

Titin and nebulin content in human skeletal muscle following eccentric resistance exercise

Protein supplementation before and after resistance training in older men

Changes in human skeletal muscle contractility and hormone status during 2 weeks of heavy strength training

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