Urinary malondialdehyde as an indicator of lipid peroxidation in the diet and in the tissues

Draper, H. H.; Polensek, L.; Hadley, M.; McGirr, Larry G.

doi:10.1007/bf02534512

Cited by 159 publications

(54 citation statements)

References 14 publications

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“…These analyses found that the spin clearance rate was increased in the diabetic rats, indicating that the oxidative stress was enhanced in these animals. Moreover, the animals' spin clearance rates were positively correlated with their urinary MDA levels, which indicate the extent of lipid peroxidation, thereby serving as an index of oxidative stress in vivo [23]. A previous study also demonstrated that urinary excretion of thiobarbituric acid-reactive substances was elevated in diabetic rats [24].…”

Section: Discussionmentioning

confidence: 82%

Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes

et al. 1998

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Section: Discussionmentioning

confidence: 82%

Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes

et al. 1998

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“…Several reports have indicated that a positive relationship exists between in vivo peroxidation and urinary malondialdehyde levels (Brooks & Klamert, 1968). For instance, vitamin E-deficient rats excrete higher levels of TBARS in urine compared with vitamin E-supplemented rats (Draper et al 1984). This demonstrates that total urinary TBARS level corresponds to the increase in vivo lipid peroxidation associated with vitamin E deficiency (Lee et al 1992).…”

Section: Discussionmentioning

confidence: 85%

Short-term consumption of a high-sucrose diet has a pro-oxidant effect in rats

Busserolles

Rock

Gueux

et al. 2002

British Journal of Nutrition

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The underlying mechanisms for the detrimental consequences of a high-fructose diet in animal models are not clear. However, the possibility exists that fructose feeding facilitates oxidative damage. Thus, the aim of the present study was to assess, in weaning rats, the effect of a highsucrose diet v. starch diet for 2 weeks on oxidative stress variables. Plasma lipid levels were measured and lipid peroxidation was evaluated by urine and plasma thiobarbituric acid-reactive substances (TBARS). The susceptibilities of several tissues to peroxidation were determined in tissue homogenates after in vitro lipid peroxidation. Antioxidant defence variables were evaluated by measuring plasma and heart vitamin E levels, and heart superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. Higher plasma triacylglycerol (P,0·01) and TBARS (P,0·01) levels were found in rats fed the sucrose diet as compared with the starch-fed group, whereas plasma a-tocopherol levels were significantly decreased in the sucrose-fed group compared with the starch-fed group (P,0·01). Higher urine TBARS (P,0·01) were found in the sucrose-fed group compared with the starch-fed group, suggesting increased production of these substances from lipid peroxidation in vivo. Higher susceptibility to peroxidation in heart, thymus and pancreas was also found in the sucrose-fed group v. the starch-fed group. No statistical differences were observed for liver TBARS level between the two groups. Heart SOD activity was significantly decreased (P,0·001) in the sucrose-fed group compared with the starch-fed group, whereas heart vitamin E level and GPX activity were not different between the groups. However, the in vitro generation of superoxide radical in heart homogenate, measured by electron spin resonance detection and spin trapping, was not increased in the sucrose-fed group compared with starch-fed rats. Altogether, the results indicate that a short-term consumption of a high-sucrose diet negatively affects the balance of free radical production and antioxidant defence in rats, leading to increased lipid susceptibility to peroxidation.High-sucrose diet: Oxidative stress: Free radicals: Rats D-Fructose is a sugar that exists in foods as a simple sugar and as a component of the disaccharide sucrose, consisting of one molecule of glucose and one of fructose. Because of the use of high-fructose corn sweeteners and of sucrose in manufactured foods, the dietary consumption of fructose has increased several-fold from that present in natural foods (Henry et al. 1991). Although there is little evidence that modest amounts of fructose have detrimental effects on carbohydrate and lipid metabolism, larger doses of fructose have been associated with numerous metabolic abnormalities in human subjects and laboratory animals, suggesting that high-fructose consumption induces adverse effects on health (Hallfrisch, 1990;Henry et al. 1991). High-sucrose and high-fructose diets were used in animal models to induce the metabolic changes observed in syndrome X, a diso...

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“…These lecithins have a similar structure to membrane phospholipids, and could have favored the uptake of iron by modifying membrane fluidity or permeability. However, most of the iron taken up could not be transferred out of the cell, inducing the end-product of membranelipid peroxidation [13] .…”

Section: Discussionmentioning

confidence: 99%

Caseinophosphopeptide-Bound Iron: Protective Effect against Gut Peroxidation

Kibangou¹,

Bouhallab

Bureau

et al. 2008

Ann Nutr Metab

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Background: Peptides derived from cow’s milk proteins have in vitro protective effects on iron-induced peroxidation that could be used to prevent the side effects of iron fortification. The aim of the study was to confirm these properties in an in vivo model of gut peroxidation. Methods: Iron bound to the 1–25 phosphopeptide of β-casein [Fe-β-CPP(1–25)] was compared to an encapsulated ferric pyrophosphate (Fe-P) in the Caco-2 model. Ferrous sulfate (FeSO₄) was used as control (100 µmol/l iron, n = 6 per group). The concentration of malondialdehyde (MDA), a stable byproduct of lipid peroxidation, was used as the marker of peroxidation. Results: The lowest MDA levels were observed in cells grown with Fe-β-CPP(1–25) and the highest with Fe-P. Iron absorption of Fe-β-CPP(1–25) was higher than in the 2 other forms, due to its high cellular uptake and high basolateral transfer, while iron absorption of Fe-P showed high uptake and high cell retention. Conclusions: The enhancing effect of β-CPP(1–25) on iron absorption was associated with a protective effect against enterocyte peroxidation, perhaps due to its low storage by enterocytes. These observations support a role for specific milk components in food fortification to prevent iron deficiency.

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Urinary malondialdehyde as an indicator of lipid peroxidation in the diet and in the tissues

Cited by 159 publications

References 14 publications

Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes

Oxidative stress measurement by in vivo electron spin resonance spectroscopy in rats with streptozotocin-induced diabetes

Short-term consumption of a high-sucrose diet has a pro-oxidant effect in rats

Caseinophosphopeptide-Bound Iron: Protective Effect against Gut Peroxidation

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