Urinary Metabolites of Isoliquiritigenin in Wistar Rats using UHPLC–TOF–MS-based Xenometabolomics

Abstract

Isoliquiritigenin, a chalcone found in licorice root and many other plants, has shown potential antioxidant, estrogenic and antitumor activities. The present study was to investigate urinary metabolism of isoliquiritigenin in Wistar rats by ultra-high pressure liquid chromatography coupled to electrospray ionization TOF-MS (UHPLC-TOF-MS)-based xenometabolomics. Urine samples were collected before and after oral administration of isoliquiritigenin, and analyzed by UHPLC-TOF-MS. After deconvolution, the resulting data matrices were subjected to multivariate data analysis. Projection to latent structures discriminant analysis was performed to screen the metabolites. Fifteen urinary metabolites of isoliquiritigenin were screened out and 13 of them were further identified by Agilent MassHunter Software. The results of this work show that UHPLC-TOF-MS-based xenometabolomics was able to comprehensively identify the metabolites of phytochemicals and may represent a valuable tool for monitoring the food consumption.

Characterization of the global metabolic profile of liquiritin in rat plasma, urine, bile and feces based on UHPLC-FT-ICR MS

“…Among them, the parent drug (M0), liquiritigenin (M2) and isoliquiritigenin (M3) were conrmed by comparison with reference standards, and the other 74 metabolites were indentied according to the obtained MS/MS data. Comparing with the previous literatures, 8,19 27 potential new metabolites of liquiritin in rats including M4, M5-M12, M20, M32, M33, M51-M58 and M70-M76 were rst tentatively identied by the UHPLC-FT-ICR-MS method in this study (ESI table †).…”

Multi-Target Screening of Biological Samples Using LC–MS/MS: Focus on Chromatographic Innovations

“…To date, the superficially porous particle technology have been already applied to the determination of few drugs and metabolites in biological fluids, including whole blood, plasma, urine, or saliva [68][69][70], but up to now, no multi-target screening procedures based on this technology has been proposed. Nevertheless, this technology is plainly appropriate for the development of HPLC-MS multi-target screenings with improved performance but without the need of specific instruments compatible with pressures up to ca.…”

Pharmacokinetic and metabolism studies of bavachinin through ultra‐high‐performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry

“…The main fragmentation pathways and MS/MS spectra are presented in Figure S3, and the combined selected ion chromatograms of BVC and its metabolites are shown in Figure S4 absorption wavelengths at 275 and 320 nm, whereas that of M1 was at 375 nm. Related studies (Legette et al, 2012;Nikolic, Li, Chadwick, Pauli, & van Breemen, 2005;Tan et al, 2011) have revealed that flavonoids can convert into the corresponding isomer of chalcone both in vivo and in vitro. The UV absorption wavelength of M1 was in accordance with the characteristic UV absorption wavelength of chalcone.…”