Urinary Metabolites of Polyamines in Rats

Abstract

Urinary metabolites of polyamines in rats were studied systematically by the intraperitoneal injection of radioactive polyamines. Urinary metabolites were fractionated into 4 fractions containing non-polar and acidic compounds, acidic and neutral ampholytes, basic ampholytes and polyamines. A large amount of radioactivity was detected in the fractions containing non-polar and acidic compounds and polyamines of urine of rats injected with radioactive putrescine, while in the case of the injection of radioactive spermidine or spermine a relatively large amount of radioactivity was found in the basic ampholyte fraction as well as in the polyamine fraction. Analysis of these fractions indicated that gamma-aminobutyric acid, N-monoacetylputrescine, 2(3)-hydroxyputrescine, putreanine, N-(3-aminopropyl)-4-aminobutyric acid (isoputreanine), spermic acid, N-(3-aminopropyl), N'-(2-carboxyethyl)-1,4-diaminobutane, and N-monoacetylspermidine A and B were excreted as urinary metabolites of the polyamines in addition to putrescine, spermidine and spermine.

Assay of 2‐Hydroxyputrescine in Various Regions of Rat Brain by Gas Chromatography‐Mass Spectrometry

“…The physiological function of 2-hydroxyputrescine has not been studied in living things, and it is difficult to discuss such functions of this compound in the CNS. However, we can compare the concentration of this compound to that of putrescine, its precursor (Noto et al, 1978), in some areas of the CNS. In the cerebellum, the concentration of 2-hydroxyputrescine is very high, but that of putrescine is very low.…”

“…Putrescine is metabolized into y-aminobutyric acid (GABA) by amine oxidase (EC 1.4.3.6) in mammalian organs (Tsuji and Nakajima, 1978), and the release of GABA formed from putrescine in the CNS is enhanced by high levels of potassium . Putrescine is also metabolized in the rat into 2-hydroxyputrescine (Noto et al, 1978), which has been already isolated and identified in Psezrdutnonas cells (Tobari and Tchen,197 1). 2-Hydroxyputrescine from bovine brain has been characterized (Hasegawa et al, 1986).…”

Spontaneous Release of γ‐Aminobutyric Acid Formed from Putrescine and Its Enhanced Ca2+‐Dependent Release by High K+ Stimulation in the Brains of Freely Moving Rats

“…Under these conditions GABA and putrescine moved about 8 and 20 cm from the origin to the cathode, respectively. These column and paper electrophoretic procedures were previously reported by Noto et al (1978). GABA was visualized with 0.2% ninhydrin in acetone.…”

Formulation of N-acetylputrescine and N1-acetylspermidine in cultured human lymphocytes

“…3 paper by using different solvents: a, propan-2-ol/85% (v/v) formic acid/water (8: 1: 1, by vol.) (Noto et al, 1978;Kakimoto et al, 1969); b, propan-1-ol/triethylamine/water (85:3:15, by vol.) (Dubin & Rosenthal, 1960).…”