Urinary metabolomics analysis reveals the effect of volatile oil from Angelica sinensis on LPS‐induced inflammation rats

Abstract

Lipopolysaccharide (LPS)-induced inflammation occurs commonly and volatile oil fromAngelica sinensis (VOAS) can be used as an anti-inflammatory agent. The molecular mechanisms that allow the anti-inflammatory factors to be expressed are still unknown. In this paper, we applied gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-time-of-flight mass spectrometry (LC-Q/TOF-MS) based on a metabolomics platform coupled with a network approach to analyze urine samples in three groups of rats: one with LPS-induced inflammation (MI); one with intervention with VOAS; and normal controls (NC). Our study found definite metabolic footprints of inflammation and showed that all three groups of rats, MI, intervention with VOAS and NC have distinct metabolic profiles in urine. The concentrations of 48 metabolites differed significantly among the three groups. The metabolites in urine were screened by the GC-MS and LC-Q/TOF-MS methods.The significantly changed metabolites (p < 0.05, variable importance in projection > 1.5) between MI, NC and VOAS were included in the metabolic networks.Finally, hub metabolites were screened, including glycine, arachidonic acid, L-glutamate, pyruvate and succinate, which have high values of degree (k). the Results suggest that disorders of glycine, arachidonic acid, L-glutamate, pyruvate and succinate metabolism might play an important part in the predisposition and development of LPS-induced inflammation. By applying metabolomics with network methods, the mechanisms of diseases are clearly elucidated.

Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

“…One of the meridian tropisms of AS is the liver. The effects of AS include replenishing blood (Yang et al, 2021 ), promoting blood circulation (Yuan et al, 2019 ) and anti‐inflammation (Hua et al, 2019 ). Polysaccharide is an essential active component in AS.…”

Anti-platelet aggregation activities of different grades of Angelica sinensis and their therapeutic mechanisms in rats with blood deficiency: insights from metabolomics and lipidomics analyses

“…Glutamine can be enzymatically broken down by glutaminase into glutamic acid and α-ketoglutaric acid, which in turn provide energy for the TCA cycle ( Hao and Yan, 2015 ). The TCA cycle, a convergence point for the catabolism of sugars, fats, and amino acids, draws on intermediates from various biosynthetic pathways ( Hua, 2014 ). Consequently, changes in the TCA cycle may reflect shifts in overall energy metabolism.…”

The Mechanism and Research Progress of Angelica Sinensis in Preventing and Treating Diabetes Nephropathy

“…②Angelica sinensis volatile oil exerts antihypertensive effects by antagonizing the ACE system through the ACE2/[Ang (1-7)]/Maas receptor axis ③Angelica sinensis volatile oil reduces blood pressure by reducing serum renin and angiotensin II levels.④Angelica sinensis volatile oil reduces blood pressure by reducing the abundance of intestinal proteus and ferritobacter. ⑤ Angelica sinensis volatile oil improves blood pressure by regulating the PI3K/Akt/eNOS signaling pathway [15] .…”