Urinary organic acid screening by solid-phase microextraction of the methyl esters

Liebich, H.M.; Gesele, Elke; Woll, J.P. Pilkington

doi:10.1016/s0378-4347(98)00156-x

Cited by 33 publications

(11 citation statements)

References 12 publications

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“…While stirring, about 20 mg of sodium carbonate were added at room temperature. Derivatization was performed in five steps as indicated by Liebich et al (1998). Within 4 min approximately 30 mg of solid trimethyloxonium tetrafluoroborate was added in five aliquots by a spatula.…”

Section: Methodsmentioning

confidence: 99%

“…These methods employ a sample derivatization technique to convert such polar substances into hydrophobic compounds whose volatility is sufficiently high for a GC determination. (Liebich et al, 1998;Liebich and Gesele, 1999;Sonntag et al, 2004). The applications of trimethyloxonium tetrafluoroborate as a methylating agent were implemented in this work.…”

Section: Introductionmentioning

confidence: 99%

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Determination of organic acids in urine by solid‐phase microextraction and gas chromatography–ion trap tandem mass spectrometry previous ‘in sample’ derivatization with trimethyloxonium tetrafluoroborate

Pacenti

Dugheri

Villanelli

et al. 2008

Biomedical Chromatography

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A method for the determination of the organic acids directly in the urine employing derivatization with trimethyloxonium tetrafluoroborate as a methylating agent and sequential extraction by head space and direct immersion/solid phase microextraction is reported. Furoic acid, hippuric acid, methylhippuric acid, mandelic acid, phenylglyoxylic acid and trans, trans muconic acid contained in urine and proposed by the American Conference of Governmental Industrial Hygienists as biological exposure indices were determined after a fast and economically convenient preparation step and sensitive gas chromatography-ion trap-mass spectrometry/tandem mass spectrometry analysis. Urine is rather a complex sample and hence the acquisition method required specific GC-MS instrumentation capable of supporting the changeover, fully automated during a single chromatographic separation, from mass to tandem mass spectrometry and both chemical and electron ionization modes. The automation of the analytical method provides a number of advantages, including reduced analysis time for both routine analysis and method development, and greater reproducibility. The equilibrium and kinetics of this substances vs head space/direct immersion-solid phase microextraction were investigated and evaluated theoretically.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of organic acids in urine by solid‐phase microextraction and gas chromatography–ion trap tandem mass spectrometry previous ‘in sample’ derivatization with trimethyloxonium tetrafluoroborate

Pacenti

Dugheri

Villanelli

et al. 2008

Biomedical Chromatography

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show abstract

“…The process can be performed in situ prior to analysis, directly in SPME. Using in situ derivatization, 29 organic acids were analyzed in urine-derivatization using trimethyloxonium tetrafluoroborate [218] or benzodiazepines after the acid hydrolysis of the corresponding glucuronides in urine [219].…”

Section: Solid Phase Microextraction (Spme)mentioning

confidence: 99%

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

Nováková

Vlčková

2009

Analytica Chimica Acta

504

237

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“…Chromatographic methods such as high-performance liquid chromatography (HPLC) [2], ion chromatography (IC) [3], gas chromatography (GC) [4,5], and ion-exclusion chromatography [6][7][8] have been used for the determination of organic acids. Among them, HPLC and IC are the most popular methods, since GC analysis requires additional derivatization procedures to enhance sample volatility.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

et al. 2003

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The parameters influencing the electrochromatographic separation of aliphatic organic acids in a capillary column with a wall-coated macrocyclic polyamine have been studied. Indirect detection using chromate, pyromellitate, trimellitate, o-phthalate, benzoate and acetate as background electrolytes has been tested. A complete separation of polyprotic acids could be achieved with pyromellitate buffer (7.5 mM, pH 6.5), and satisfactory results for the simultaneous separation of monoprotic acids and polyprotic acids were found using a capillary column of 70 cm (50 cm effective length)x75 microm inner diameter, electrokinetic injection (-10 kV, 10 s), benzoate buffer (6 mM, pH 4.6), separation voltage of -10 kV, and detection at 220 nm. For the separation of the geometric isomers fumarate and maleate, acetate buffer was found the best choice among the background electrolytes tested. The method so established has been applied to the determination of organic acids in soy sauce, brandy, lemon juice, spinach juice and cigarette. From the retention behavior, it was found that the separation mechanism on the bonded phase was influenced by the macrocyclic effect, electrostatic attraction, hydrogen bonding, van der Waals forces, and anion exchange, in addition to the differences in electrophoretic mobility.

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Urinary organic acid screening by solid-phase microextraction of the methyl esters

Cited by 33 publications

References 12 publications

Determination of organic acids in urine by solid‐phase microextraction and gas chromatography–ion trap tandem mass spectrometry previous ‘in sample’ derivatization with trimethyloxonium tetrafluoroborate

Determination of organic acids in urine by solid‐phase microextraction and gas chromatography–ion trap tandem mass spectrometry previous ‘in sample’ derivatization with trimethyloxonium tetrafluoroborate

A review of current trends and advances in modern bio-analytical methods: Chromatography and sample preparation

Capillary electrochromatographic analysis of aliphatic mono‐ and polycarboxylic acids

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