Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis

El-Kasti, Muna M.; Wright, Cynthia; Fye, Haddy K. S.; Roseman, Fenella; Kessler, Benedikt M.; Becker, Christian M.

doi:10.1016/j.fertnstert.2010.11.066

Cited by 39 publications

(20 citation statements)

References 44 publications

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“…Using MALDI-TOF MS (matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) and time-of-flight analyzer (TOF)), differential peptide profiles have been described in the urine of endometriotic women, compared to healthy subjects. Tokushige and colleagues [144], revealed a 12-fold higher expression of five proteins in affected women by combining MALDI-TOF with two-dimensional polyacrylamide gel electrophoresis and El-Kasti et al [145] highlighted a 3280.9 Da periovulatory peptide that differentiates women with all stages of endometriosis from healthy subjects (sensitivity 82% and specificity 88%).…”

Section: Urinary Biomarkers In Endometriosismentioning

confidence: 99%

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Anastasiu

Moga

Neculau

et al. 2020

IJMS

125

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Background: Early and accurate diagnosis of endometriosis is crucial for the management of this benign, yet debilitating pathology. Despite the advances of modern medicine, there is no common ground regarding the pathophysiology of this disease as it continues to affect the quality of life of millions of women of reproductive age. The lack of specific symptoms often determines a belated diagnosis. The gold standard remains invasive, surgery followed by a histopathological exam. A biomarker or a panel of biomarkers is easy to measure, usually noninvasive, and could benefit the clinician in both diagnosing and monitoring the treatment response. Several studies have advanced the idea of biomarkers for endometriosis, thereby circumventing unnecessary invasive techniques. Our paper aims at harmonizing the results of these studies in the search of promising perspectives on early diagnosis. Methods: We selected the papers from Google Academic, PubMed, and CrossRef and reviewed recent articles from the literature, aiming to evaluate the effectiveness of various putative serum and urinary biomarkers for endometriosis. Results: The majority of studies focused on a panel of biomarkers, rather than a single biomarker and were unable to identify a single biomolecule or a panel of biomarkers with sufficient specificity and sensitivity in endometriosis. Conclusion: Noninvasive biomarkers, proteomics, genomics, and miRNA microarray may aid the diagnosis, but further research on larger datasets along with a better understanding of the pathophysiologic mechanisms are needed.

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Section: Urinary Biomarkers In Endometriosismentioning

confidence: 99%

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Anastasiu

Moga

Neculau

et al. 2020

IJMS

125

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show abstract

“…Lower antimullerian hormone serum levels and an association with the severity were found in women with endometriosis. Others have found that urinary proteomic analysis may provide a novel method of diagnosing and staging endometriosis [43 ]. Leptin concentrations in the peritoneal fluid of women with ovarian endometriosis have been found to be different according to the presence of a 'deep' or 'superficial' ovarian disease, suggesting that leptin could play an active role in promoting the development of 'superficial' ovarian endometriomas and that 'superficial' and 'deep' ovarian endometriomas could have a different pathogenesis [41].…”

Section: Current Considerations For Classifying Endometriosismentioning

confidence: 99%

Endometriosis classification

Adamson

2011

Current Opinion in Obstetrics &Amp; Gynecology

106

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“…The urine proteome has also been studied in the context of endometriosis, a biospecimen of particular interest in the search for clinically useful biomarkers. One group used MALDI TOF MS to identify disease-specific urinary peptide profiles [48]. In addition, MS approaches identified several isoforms of cytokeratin 19 [9] and matrix metalloproteinase [49] as upregulated in urine from women with biopsy-confirmed endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Urine, peritoneal fluid and omental fat proteomes of reproductive age women: Endometriosis-related changes and associations with endocrine disrupting chemicals

Williams

Miroshnychenko

Johansen

et al. 2015

Journal of Proteomics

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Endometriosis, ectopic growth of the uterine lining (endometrium), which affects 6–11% of reproductive age women, is associated with pelvic pain and infertility. We investigated the peritoneal fluid (PF), urine and omental fat (OF) proteomes of women with endometriosis vs. individuals with no surgically visualized endometriosis. All participants were enrolled in the NICHD-funded ENDO Study. A two-step proteomic study was performed. The first, a broad survey, employed a semi-quantitative gel LC-mass spectrometry (MS) workflow: SDS PAGE fractionation, trypsin digestion and LC–MS/MS. The results showed sample integrity but failed to detect any differences between women with and without endometriosis. The second step was a quantitative analysis of OF samples. We employed another sample set (n = 30) from women ± disease and isobaric mass-tag (iTRAQ) chemistry to label peptides and 2D LC–MS/MS for protein identification and quantification. Three proteins—matrix metalloproteinase-9, neutrophil elastase, and FAM49B—were significantly lower in abundance in samples from women with endometriosis. Interestingly, neutrophil elastase and FAM49B levels were associated with higher levels of a subset of endocrine disrupting chemicals (EDCs) that were previously measured in the same samples. The results of these experiments showed the feasibility of associating endometriosis with changes in the OF protein repertoire and EDC levels. Biological significance Endometriosis, pathological growth of the uterine lining, is associated with significant morbidities, including pain and infertility. However, the causes of this common condition are poorly understood. This study determined whether endometriosis was associated with changes in the protein composition of peritoneal fluid, urine and/or omental fat. A protein of unknown function (FAM49B) and two proteinases (metalloproteinase-9, neutrophil elastase) were down regulated in OF samples from women with versus without endometriosis. These findings suggested proteinase imbalances at sites that were distant from the endometriotic lesions. Additionally, FAM49B and neutrophil elastase levels were associated with higher levels of a subset of environmental chemicals that were quantified in the same samples, suggesting other possible associations. Thus, this work generated hypotheses that will be tested in further studies.

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Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis

Cited by 39 publications

References 44 publications

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Biomarkers for the Noninvasive Diagnosis of Endometriosis: State of the Art and Future Perspectives

Endometriosis classification

Urine, peritoneal fluid and omental fat proteomes of reproductive age women: Endometriosis-related changes and associations with endocrine disrupting chemicals

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